Construct: ORF TRCN0000487907
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020628.1_s317c1
- DNA Barcode:
- CGCGCTCTGGAGTCTCGCTTGACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TYK2 (7297)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487907
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7297 | TYK2 | tyrosine kinase 2 | NM_003331.5 | 99.9% | 99.9% | 3046G>T |
| 2 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528245.1 | 99.9% | 99.9% | 3046G>T |
| 3 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528246.3 | 91.6% | 91.5% | 0_1ins297;2749G>T |
| 4 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528247.1 | 91.6% | 91.5% | 0_1ins297;2749G>T |
| 5 | human | 7297 | TYK2 | tyrosine kinase 2 | XR_001753750.1 | 85.4% | (many diffs) | |
| 6 | human | 7297 | TYK2 | tyrosine kinase 2 | XR_001753751.1 | 75% | (many diffs) | |
| 7 | human | 7297 | TYK2 | tyrosine kinase 2 | XR_002958353.1 | 62.9% | (many diffs) | |
| 8 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528249.2 | 62.4% | 61.7% | (many diffs) |
| 9 | human | 7297 | TYK2 | tyrosine kinase 2 | XR_001753752.1 | 61.4% | 1_157del;2205_2316del;2623_2624ins1207 | |
| 10 | human | 7297 | TYK2 | tyrosine kinase 2 | XM_011528251.1 | 51% | 45.7% | 0_1ins1624;146_147ins119;1303G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3633
- ORF length:
- 3561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcctctg cgccactggg ggatggccag gggcagtaag cccgttgggg 121 atggagccca gcccatggct gccatgggag gcctgaaggt gcttctgcac tgggctggtc 181 caggcggcgg ggagccctgg gtcactttca gtgagtcatc gctgacagct gaggaagtct 241 gcatccacat tgcacataaa gttggtatca ctcctccttg cttcaatctc tttgccctct 301 tcgatgctca ggcccaagtc tggttgcccc caaaccacat cctagagatc cccagagatg 361 caagcctgat gctatatttc cgcataaggt tttatttccg gaactggcat ggcatgaatc 421 ctcgggaacc ggctgtgtac cgttgtgggc ccccaggaac cgaggcatcc tcagatcaga 481 cagcacaggg gatgcaactc ctggacccag cctcatttga gtacctcttt gagcagggca 541 agcatgagtt tgtgaatgac gtggcatcac tgtgggagct gtcgaccgag gaggagatcc 601 accactttaa gaatgagagc ctgggcatgg cctttctgca cctctgtcac ctcgctctcc 661 gccatggcat ccccctggag gaggtggcca agaagaccag cttcaaggac tgcatcccgc 721 gctccttccg ccggcatatc cggcagcaca gcgccctgac ccggctgcgc cttcggaacg 781 tcttccgcag gttcctgcgg gacttccagc cgggccgact ctcccagcag atggtcatgg 841 tcaaatacct agccacactc gagcggctgg caccccgctt cggcacagag cgtgtgcccg 901 tgtgccacct gaggctgctg gcccaggccg agggggagcc ctgctacatc cgggacagtg 961 gggtggcccc tacagaccct ggccctgagt ctgctgctgg gcccccaacc cacgaggtgc 1021 tggtgacagg cactggtggc atccagtggt ggccagtaga ggaggaggtg aacaaggagg 1081 agggttctag tggcagcagt ggcaggaacc cccaagccag cctgtttggg aagaaggcca 1141 aggctcacaa ggcagtcggc cagccggcag acaggccgcg ggagccactg tgggcctact 1201 tctgtgactt ccgggacatc acccacgtgg tgctgaaaga gcactgtgtc agcatccacc 1261 ggcaggacaa caagtgcctg gagctgagct tgccttcccg ggctgcggcg ctgtccttcg 1321 tgtcgctggt ggacggctat ttccgcctga cggccgactc cagccactac ctgtgccacg 1381 aggtggctcc cccacggctg gtgatgagca tccgggatgg gatccacgga cccctgctgg 1441 agccatttgt gcaggccaag ctgcggcccg aggacggcct gtacctcatt cactggagca 1501 ccagccaccc ctaccgcctg atcctcacag tggcccagcg tagccaggca ccagacggca 1561 tgcagagctt gcggctccga aagttcccca ttgagcagca ggacggggcc ttcgtgctgg 1621 agggctgggg ccggtccttc cccagcgttc gggaacttgg ggctgccttg cagggctgct 1681 tgctgagggc cggggatgac tgcttctctc tgcgtcgctg ttgcctgccc caaccaggag 1741 aaacctccaa tctcatcatc atgcgggggg ctcgggccag ccccaggaca ctcaacctca 1801 gccagctcag cttccaccgg gttgaccaga aggagatcac ccagctgtcc cacttgggcc 1861 agggcacaag gaccaacgtg tatgagggcc gcctgcgagt ggagggcagc ggggaccctg 1921 aggagggcaa gatggatgac gaggaccccc tcgtgcctgg cagggaccgt gggcaggagc 1981 tacgagtggt gctcaaagtg ctggacccta gtcaccatga catcgccctg gccttctacg 2041 agacagccag cctcatgagc caggtctccc acacgcacct ggccttcgtg catggcgtct 2101 gtgtgcgcgg ccctgaaaat atcatggtga cagagtacgt ggagcacgga cccctggatg 2161 tgtggctgcg gagggagcgg ggccatgtgc ccatggcttg gaagatggtg gtggcccagc 2221 agctggccag cgccctcagc tacctggaga acaagaacct ggttcatggt aatgtgtgtg 2281 gccggaacat cctgctggcc cggctggggt tggcagaggg caccagcccc ttcatcaagc 2341 tgagtgatcc tggcgtgggc ctgggcgccc tctccaggga ggagcgggtg gagaggatcc 2401 cctggctggc ccccgaatgc ctaccaggtg gggccaacag cctaagcacc gccatggaca 2461 agtgggggtt tggcgccacc ctcctggaga tctgctttga cggagaggcc cctctgcaga 2521 gccgcagtcc ctccgagaag gagcatttct accagaggca gcaccggctg cccgagccct 2581 cctgcccaca gctggccaca ctcaccagcc agtgtctgac ctatgagcca acccagaggc 2641 catcattccg caccatcctg cgtgacctca cccggctgca gccccacaat cttgctgacg 2701 tcttgactgt gaacccggac tcaccggcgt cggaccctac ggttttccac aagcgctatt 2761 tgaaaaagat ccgagatctg ggcgagggtc acttcggcaa ggtcagcttg tactgctacg 2821 atccgaccaa cgacggcact ggcgagatgg tggcggtgaa agccctcaag gcagactgcg 2881 gcccccagca ccgctcgggc tggaagcagg agattgacat tctgcgcacg ctctaccacg 2941 agcacatcat caagtacaag ggctgctgcg aggaccaagg cgagaagtcg ctgcagctgg 3001 tcatggagta cgtgcccctg ggcagcctcc gagactacct gccccggcac agcatcgggc 3061 tggcccagct gctgctcttc gcccagcaga tctgcgaggg catggcctat ctgcactcgc 3121 agcactacat ccaccgagac ctagccgcgc gcaacgtgct gctggacaac gacaggctgg 3181 tcaagatcgg ggactttggc ctagccaagg ccgtgcccga aggccacgag tactaccgcg 3241 tgcgcgagga tggggacagc cccgtgttct ggtatgcccc agagtgcctg aaggagtata 3301 agttctacta tgcgtcagat gtctggtccT TCGGGGTGAC CCTGTATGAG CTGCTGACGC 3361 ACTGTGACTC CAGCCAGAGC CCCCCCACGA AATTCCTTGA GCTCATAGGC ATTGCTCAGG 3421 GTCAGATGAC AGTTCTGAGA CTCACTGAGT TGCTGGAACG AGGGGAGAGG CTGCCACGGC 3481 CCGACAAATG TCCCTGTGAG GTCTATCATC TCATGAAGAA CTGCTGGGAG ACAGAGGCGT 3541 CCTTTCGCCC AACCTTCGAG AACCTCATAC CCATTCTGAA GACAGTCCAT GAGAAGTACC 3601 AAGGCCAGGC CCCTTCAGTG TTCAGCGTGT GCTAGAACCC AGCTTTCTTG TACAAAGTGG 3661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3781 ACGCGCTCTG GAGTCTCGCT TGACAACGCG TTAAGTCgac aatcaacctc tggattacaa 3841 aatttgtgaa agatt