Construct: ORF TRCN0000487914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021197.1_s317c1
- DNA Barcode:
- TAGTGAAAACACATTTGAACTACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GALR3 (8484)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487914
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8484 | GALR3 | galanin receptor 3 | NM_003614.2 | 81.4% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1176
- ORF length:
- 1104
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgac gcccagaaca tcagcctgga cagccctggc agcgtgggag 121 ctgtggctgt gcctgtggtg ttcgccctga tcttcctgct gggcaccgtg ggcaacggcc 181 tggtgctggc tgtgctgctg cagcctggac catccgcctg gcaggaacct ggcagcacca 241 ccgacctgtt catcctgaac ctggccgtgg ccgacctgtg cttcatcctg tgctgcgtgc 301 cattccaggc caccatctac accctggacg cctggctgtt cggcgccctc gtgtgcaagg 361 ccgtgcatct gctgatctac ctgactatgt acgccagctc cttcaccctc gctgctgtgt 421 ccgtggaccg gtatctggcc gtgcggcacc ccctgagaag cagagccctg cggaccccca 481 gaaacgccag agctgccgtg ggcctcgtgt ggctgctggc cgccctgttt agcgccccct 541 acctgagcta ctacggcaca gtgcgctacg gcgccctgga actgtgtgtg cccgcctggg 601 aggacgccag acgcagagca ctggacgtgg ccaccttcgc cgctggctac ctgctccctg 661 tcgccgtggt gtctctggcc tacggccgga ccctgcggtt tctgtgggcc gctgtgggac 721 ctgctggcgc agcagccgct gaagccagaa gaagggccac aggcagagcc ggcagggcta 781 tgctggcagt ggccgctctg tacgccctgt gttggggacc tcaccacgcc ctgatcctgt 841 gcttttggta cggcagattc gccttcagcc ccgccaccta cgcctgcaga ctggcctctc 901 actgcctggc cTACGCCAAC AGCTGCCTGA ACCCCCTGGT GTATGCCCTG GCCAGCCGGC 961 ACTTCCGGGC CAGATTCAGA CGGCTGTGGC CCTGCGGCAG ACGGCGGAGA CACAGAGCTA 1021 GACGGGCCCT GCGGAGAGTG CGGCCTGCCT CTTCTGGCCC TCCTGGCTGT CCTGGAGACG 1081 CCAGACCTAG CGGCAGACTG CTGGCTGGCG GCGGACAGGG ACCCGAGCCT AGAGAAGGAC 1141 CTGTGCACGG CGGCGAGGCC GCCAGAGGAC CTGAATGAGA CCCAGCTTTC TTGTACAAAG 1201 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1261 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1321 ACGATAGTGA AAACACATTT GAACTACAAC GCGTTAAGTC gacaatcaac ctctggatta 1381 caaaatttgt gaaagatt