Construct: ORF TRCN0000487930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021595.1_s317c1
- DNA Barcode:
- ATGCCCCACTGGCGGGAGCGTTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KIF12 (113220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113220 | KIF12 | kinesin family member 12 | NM_138424.1 | 100% | 100% | |
| 2 | human | 113220 | KIF12 | kinesin family member 12 | XM_024447404.1 | 100% | 100% | |
| 3 | human | 113220 | KIF12 | kinesin family member 12 | XM_006716947.2 | 80.7% | 74.1% | 1_312del;1715_1734del;1872_1905del |
| 4 | human | 113220 | KIF12 | kinesin family member 12 | XM_024447408.1 | 78.8% | 78.8% | 1_414del |
| 5 | human | 113220 | KIF12 | kinesin family member 12 | XM_024447406.1 | 78.4% | 76.9% | (many diffs) |
| 6 | human | 113220 | KIF12 | kinesin family member 12 | XM_005251683.5 | 76.6% | 70.3% | 1_414del;1817_1836del;1974_2007del |
| 7 | human | 113220 | KIF12 | kinesin family member 12 | XM_024447405.1 | 76.5% | 73.3% | (many diffs) |
| 8 | human | 113220 | KIF12 | kinesin family member 12 | XR_002956749.1 | 66.9% | (many diffs) | |
| 9 | human | 113220 | KIF12 | kinesin family member 12 | XR_002956751.1 | 66.2% | 1_219del;1401_1566del;1660_1661ins264 | |
| 10 | human | 113220 | KIF12 | kinesin family member 12 | XR_002956750.1 | 64.1% | (many diffs) | |
| 11 | human | 113220 | KIF12 | kinesin family member 12 | XM_024447407.1 | 44.6% | 24.6% | 324_325ins157;651_731del;804_805ins659 |
| 12 | mouse | 16552 | Kif12 | kinesin family member 12 | XM_017320000.1 | 39.7% | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1608
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcagaggacc ttcgcctggc tgttggaccg cgtgcagcac ctgggtgccc 121 ctgtcaccct tcgcgcctct tatctggaga tctacaatga gcaggttcgg gacttgctga 181 gcctggggtc tccccggccc ctccctgttc gctggaacaa gactcggggc ttctatgtgg 241 agcagctgcg ggtggtggaa tttgggagtc tggaggccct gatggaactt ttgcaaacgg 301 gtctcagccg tcgaaggaac tcagcccaca ccctgaacca ggcctccagc cgaagccatg 361 ccctgctcac cctttacatc agccgtcaaa ctgcccagca gatgccttct gtggaccctg 421 gggagccccc tgttggtggg aagctgtgct ttgtggacct ggcaggcagt gagaaggtag 481 cagccacggg atcccgtggg gagctgatgc ttgaggctaa cagcatcaac cgaagcctgc 541 tggccctggg tcactgcatc tccctgctgc tggacccaca gcggaagcag agccacatcc 601 ctttccggga cagcaagctc accaagttgc tggcagactc actgggaggg cgcggggtca 661 ccctcatggt ggcctgcgtg tccccctcag cccagtgcct tcctgagact ctcagcaccc 721 tgcgatatgc aagccgagct cagcgggtca ccacccgacc acaggccccc aagtctcctg 781 tggcaaagca gccccagcgt ttggagacag agatgctgca gctccaggag gagaaccgtc 841 gcctgcagtt ccagctggac caaatggact gcaaggcctc agggctcagt ggagcccggg 901 tggcctgggc ccagcggaac ctgtacggga tgctacagga gttcatgcta gagaatgaga 961 ggctcaggaa agaaaagagc cagctgcaga atagccgaga cctggcccag aatgagcagc 1021 gcatcctggc ccagcaggtc catgcactag agaggcgtct cctctctgcc tgctaccatc 1081 accagcaggg tcctggcctg accccaccgt gtccctgctt gatggcccca gctccccctt 1141 gccatgcact gccacccctc tactcctgcc cctgctgcca catctgccca ctgtgtcgag 1201 tgcccctggc ccactgggcc tgcctgccag gggagcacca cctgccccag gtgttggacc 1261 ctgaggcctc aggtggcagg cccccatctg cccggccccc accctgggca cccccatgca 1321 gccctggctc tgccaagtgc ccaagagaga ggagtcacag tgactggacT CAGACCCGAG 1381 TCCTGGCAGA GATGTTGACG GAGGAGGAGG TGGTACCTTC TGCACCTCCC CTGCCTGTGA 1441 GGCCCCCGAA GACATCACCA GGGCTCAGAG GTGGGGCCGG GGTTCCAAAC CTGGCCCAGA 1501 GACTGGAGGC CCTCAGAGAC CAGATTGGCA GCTCCCTGCG ACGTGGCCGC AGCCAGCCAC 1561 CCTGCAGTGA GGGCGCACGG AGCCCAGGCC AAGTCCTCCC TCCCCATTAG AACCCAGCTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGAATGC CCCACTGGCG GGAGCGTTCG ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt