Construct: ORF TRCN0000487944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021106.1_s317c1
- DNA Barcode:
- AAATGTCACGCTGACTGCTTTATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FZD6 (8323)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487944
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001164615.2 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_003506.4 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001164616.2 | 95.3% | 95.3% | (many diffs) |
| 4 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001317796.2 | 56.2% | 55.4% | (many diffs) |
| 5 | human | 8323 | FZD6 | frizzled class receptor 6 | NR_133921.2 | 51.7% | (many diffs) | |
| 6 | mouse | 14368 | Fzd6 | frizzled class receptor 6 | XM_011245455.2 | 57.3% | 59% | (many diffs) |
| 7 | mouse | 14368 | Fzd6 | frizzled class receptor 6 | XM_017316431.1 | 57.3% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2190
- ORF length:
- 2118
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagatg tttacatttt tgttgacgtg tatttttcta cccctcctaa 121 gagggcacag tctcttcacc tgtgaaccaa ttactgttcc cagatgtatg aaaatggcct 181 acaacatgac gtttttccct aatctgatgg gtcattatga ccagagtatt gccgcggtgg 241 aaatggagca ttttcttcct ctcgcaaatc tggaatgttc accaaacatt gaaactttcc 301 tctgcaaagc atttgtacca acctgcatag aacaaattca tgtggttcca ccttgtcgta 361 aactttgtga gaaagtatat tctgattgca aaaaattaat tgacactttt gggatccgat 421 ggcctgagga gcttgaatgt gacagattac aatactgtga tgagactgtt cctgtaactt 481 ttgatccaca cacagaattt cttggtcctc agaagaaaac agaacaagtc caaagagaca 541 ttggattttg gtgtccaagg catcttaaga cttctggggg acaaggatat aagtttctgg 601 gaattgacca gtgtgcgcct ccatgcccca acatgtattt taaaagtgat gagctagagt 661 ttgcaaaaag ttttattgga acagtttcaa tattttgtct ttgtgcaact ctgttcacat 721 tccttacttt tttaattgat gttagaagat tcagataccc agagagacca attatatatt 781 actctgtctg ttacagcatt gtatctctta tgtacttcat tggatttttg ctgggcgata 841 gcacagcctg caataaggca gatgagaagc tagaacttgg tgacactgtt gtcctaggct 901 ctcaaaataa ggcttgcacc gttttgttca tgcttttgta ttttttcaca atggctggca 961 ctgtgtggtg ggtgattctt accattactt ggttcttagc tgcaggaaga aaatggagtt 1021 gtgaagccat cgagcaaaaa gcagtgtggt ttcatgctgt tgcatgggga acaccaggtt 1081 tcctgactgt tatgcttctt gctctgaaca aagttgaagg agacaacatt agtggagttt 1141 gctttgttgg cctttatgac ctggatgctt ctcgctactt tgtactcttg ccactgtgcc 1201 tttgtgtgtt tgttgggctc tctcttcttt tagctggcat tatttcctta aatcatgttc 1261 gacaagtcat acaacatgat ggccggaacc aagaaaaact aaagaaattt atgattcgaa 1321 ttggagtctt cagcggcttg tatcttgtgc cattagtgac acttctcgga tgttacgtct 1381 atgagcaagt gaacaggatt acctgggaga taacttgggt ctctgatcat tgtcgtcagt 1441 accatatccc atgtccttat caggcaaaag caaaagctcg accagaattg gctttattta 1501 tgataaaata cctgatgaca ttaattgttg gcatctctgc tgtcttctgg gttggaagca 1561 aaaagacatg cacagaatgg gctgggtttt ttaaacgaaa tcgcaagaga gatccaatca 1621 gtgaaagtcg aagagtacta caggaatcat gtgagttttt cttaaagcac aattctaaag 1681 ttaaacacaa aaagaagcac tataaaccaa gttcacacaa gctgaaggtc atttccaaat 1741 ccatgggaac cagcacagga gctacagcaa atcatggcac ttctgcagta gcaattacta 1801 gccatgatta cctaggacaa gaaactttga cagaaatcca aaCCTCACCA GAAACATCAA 1861 TGAGAGAGGT GAAAGCGGAC GGAGCTAGCA CCCCCAGGTT AAGAGAACAG GACTGTGGTG 1921 AACCTGCCTC GCCAGCAGCA TCCATCTCCA GACTCTCTGG GGAACAGGTC GACGGGAAGG 1981 GCCAGGCAGG CAGTGTATCT GAAAGTGCGC GGAGTGAAGG AAGGATTAGT CCAAAGAGTG 2041 ATATTACTGA CACTGGCCTG GCACAGAGCA ACAATTTGCA GGTCCCCAGT TCTTCAGAAC 2101 CAAGCAGCCT CAAAGGTTCC ACATCTCTGC TTGTTCACCC AGTTTCAGGA GTGAGAAAAG 2161 AGCAGGGAGG TGGTTGTCAT TCAGATACTT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 2221 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2281 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 2341 ATGTCACGCT GACTGCTTTA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2401 ttgtgaaaga tt