Construct: ORF TRCN0000487948
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020147.1_s317c1
- DNA Barcode:
- TGTCTAAAATTCGGACATACTTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TWF1 (5756)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487948
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5756 | TWF1 | twinfilin actin binding pro... | NM_002822.5 | 100% | 100% | |
| 2 | human | 5756 | TWF1 | twinfilin actin binding pro... | NM_001242397.2 | 98% | 98% | 483_503del |
| 3 | human | 5756 | TWF1 | twinfilin actin binding pro... | NR_073473.2 | 34.5% | 1_72del;96_150del;1178_3042del | |
| 4 | human | 5756 | TWF1 | twinfilin actin binding pro... | NR_073472.2 | 30.5% | (many diffs) | |
| 5 | mouse | 19230 | Twf1 | twinfilin actin binding pro... | NM_008971.4 | 88.9% | 94.8% | (many diffs) |
| 6 | mouse | 19230 | Twf1 | twinfilin actin binding pro... | XM_006520638.1 | 82.1% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1122
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcccac cagaccggca tccaagcaag tgaagatgtt aaagagatct 121 ttgccagagc cagaaatgga aagtacagac ttctgaaaat atctattgaa aatgagcaac 181 ttgtgattgg atcatatagt cagccttcag attcctggga taaggattat gattcctttg 241 ttttacccct gttggaggac aaacaaccat gctatatatt attcaggtta gattctcaga 301 atgcccaggg atatgaatgg atattcattg catggtctcc agatcattct catgttcgtc 361 aaaaaatgtt gtatgcagca acaagagcaa ctctgaagaa ggaatttgga ggtggccaca 421 ttaaagatga agtatttgga acagtaaagg aagatgtatc attacatgga tataaaaaat 481 acttgctgtc acaatcttcc cctgccccac tgactgcagc tgaggaagaa ctacgacaga 541 ttaaaatcaa tgaggtacag actgacgtgg gtgtggacac taagcatcaa acactacaag 601 gagtagcatt tcccatttct cgagaagcct ttcaggcttt ggaaaaattg aataatagac 661 agctcaacta tgtgcagttg gaaatagata taaaaaatga aattataatt ttggccaaca 721 caacaaatac agaactgaaa gatttgccaa agaggattcc caaggattca gctcgttacc 781 atttctttct gtataaacat tcccatgaag gagactattt agagtccata gtttttattt 841 attcaatgcc tGGATACACA TGCAGTATAA GAGAGCGGAT GCTGTATTCT AGCTGCAAGA 901 GCCGTCTGCT AGAAATTGTA GAAAGACAAC TACAAATGGA TGTAATTAGA AAGATCGAGA 961 TAGACAATGG GGATGAGTTG ACTGCAGACT TCCTTTATGA AGAAGTACAT CCCAAGCAGC 1021 ATGCACACAA GCAAAGTTTT GCAAAACCAA AAGGTCCTGC AGGAAAAAGA GGAATTCGAA 1081 GACTAATTAG GGGCCCAGCG GAAACTGAAG CTACTACTGA TTGAGACCCA GCTTTCTTGT 1141 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1201 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1261 GAAAGGACGA TGTCTAAAAT TCGGACATAC TTAAACGCGT TAAGTCgaca atcaacctct 1321 ggattacaaa atttgtgaaa gatt