Transcript: Mouse NM_008971.4

Mus musculus twinfilin actin binding protein 1 (Twf1), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Twf1 (19230)
Length:
2973
CDS:
49..1101

Additional Resources:

NCBI RefSeq record:
NM_008971.4
NBCI Gene record:
Twf1 (19230)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008971.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023825 CCCAGGGATATGAATGGATAT pLKO.1 281 CDS 100% 10.800 15.120 N Twf1 n/a
2 TRCN0000023824 CGGGAAATACAGACTTCTGAA pLKO.1 114 CDS 100% 4.950 6.930 N Twf1 n/a
3 TRCN0000345181 CGGGAAATACAGACTTCTGAA pLKO_005 114 CDS 100% 4.950 6.930 N Twf1 n/a
4 TRCN0000006365 CCAGGGATATGAATGGATATT pLKO.1 282 CDS 100% 13.200 9.240 N TWF1 n/a
5 TRCN0000338481 CCAGGGATATGAATGGATATT pLKO_005 282 CDS 100% 13.200 9.240 N TWF1 n/a
6 TRCN0000023828 GCGACTCTGAAGAAAGAATTT pLKO.1 364 CDS 100% 13.200 9.240 N Twf1 n/a
7 TRCN0000345182 GCGACTCTGAAGAAAGAATTT pLKO_005 364 CDS 100% 13.200 9.240 N Twf1 n/a
8 TRCN0000023827 GCGTTACCATTTCTTTCTGTA pLKO.1 750 CDS 100% 4.950 3.465 N Twf1 n/a
9 TRCN0000345128 GCGTTACCATTTCTTTCTGTA pLKO_005 750 CDS 100% 4.950 3.465 N Twf1 n/a
10 TRCN0000023826 GCTGAGTAAGAAGCAGCTCAA pLKO.1 624 CDS 100% 4.050 2.835 N Twf1 n/a
11 TRCN0000345127 GCTGAGTAAGAAGCAGCTCAA pLKO_005 624 CDS 100% 4.050 2.835 N Twf1 n/a
12 TRCN0000006366 GCCACATTAAAGATGAAGTAT pLKO.1 392 CDS 100% 5.625 2.813 Y TWF1 n/a
13 TRCN0000338421 GCCACATTAAAGATGAAGTAT pLKO_005 392 CDS 100% 5.625 2.813 Y TWF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008971.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487948 TGTCTAAAATTCGGACATACTTAA pLX_317 26.3% 88.9% 94.8% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_11071 pDONR223 100% 64% 68.5% None (many diffs) n/a
3 ccsbBroad304_11071 pLX_304 0% 64% 68.5% V5 (many diffs) n/a
4 TRCN0000480877 CTTCATGTCTATGGGGTAGGACTT pLX_317 53.3% 64% 68.5% V5 (many diffs) n/a
5 ccsbBroadEn_14821 pDONR223 0% 64% 68.5% None (many diffs) n/a
6 ccsbBroad304_14821 pLX_304 0% 64% 68.5% V5 (many diffs) n/a
7 TRCN0000469729 GGGCTCTGATTAAGACCCTCTAGA pLX_317 47.4% 64% 68.5% V5 (many diffs) n/a
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