Construct: ORF TRCN0000487957
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021342.1_s317c1
- DNA Barcode:
- CTTTCCTCGTCAAGTGAGTTTGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SBNO2 (22904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487957
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22904 | SBNO2 | strawberry notch homolog 2 | NM_014963.3 | 99.9% | 99.7% | 3317C>T;3689G>C;4096C>T |
| 2 | human | 22904 | SBNO2 | strawberry notch homolog 2 | NM_001100122.2 | 94.9% | 93.5% | (many diffs) |
| 3 | human | 22904 | SBNO2 | strawberry notch homolog 2 | XM_011527804.3 | 89.3% | 88.7% | 3317C>T;3612_3613insGTGG;3663_3664ins431 |
| 4 | mouse | 216161 | Sbno2 | strawberry notch homolog 2 ... | XM_017313893.1 | 57.8% | 59.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 59
- ORF end:
- 4157
- ORF length:
- 4098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccat 61 gcttgcagtg gggcccgcca tggacaggga ttacccgcag catgaacccc cgccggcggg 121 cagcctcctg tacagcccgc cgcccctgca gagcgccatg ctgcactgcc cctactggaa 181 caccttctcg ctgccgccat accctgcctt ctccagcgac agccgcccgt tcatgagctc 241 cgcctccttc ctcggcagcc agccctgccc agacaccagc tatgcccccg tggccaccgc 301 ctccagcttg ccaccaaaga cctgcgactt tgctcaggac tcctcctatt ttgaggactt 361 ctccaacatc tccatcttct cctcgtccgt ggactccctg tcggacatcg tggacacgcc 421 cgacttcctg ccggctgaca gcctcaacca ggtgtccacc atctgggacg ataaccctgc 481 cccctccacc cacgataagc tgttccagct cagcaggccg tttgcaggct tcgaggactt 541 tctgccctcc cacagcaccc cgcttctcgt cagctaccag gagcagagtg tgcagagcca 601 gccagaggag gaggacgagg ctgaggagga ggaggcggag gagctggggc acacagagac 661 ctacgccgac tacgtgccgt ccaagtccaa gatcgggaag cagcacccag accgcgtggt 721 ggagaccagc acactgtcca gcgtcccacc cccagacatc acctacaccc tggccctgcc 781 ctcggacagc ggggccctgt ctgccctgca gctagaggcc atcacctacg cctgccagca 841 acacgaggtc ctgctcccca gcgggcagcg cgcgggcttt ctcatcggcg atggggccgg 901 cgtgggcaaa ggccggacgg tggccggagt catcctggag aaccacctgc gcggccggaa 961 gaaagcattg tggttcagcg tctccaacga cctcaagtac gatgcggagc gcgacctgcg 1021 ggacatcgaa gccacgggca tcgcggtgca cgcgctcagc aagatcaagt acggtgacac 1081 cactacctca gagggcgtcc tcttcgccac ctactccgcc ctgattgggg agagccaggc 1141 cggcggccag caccgcactc gcctccggca gatcctggac tggtgtgggg aggccttcga 1201 gggcgtcatc gtgttcgacg agtgtcacaa agccaagaat gccggctcca ccaagatggg 1261 caaggctgtg ctagacctgc agaacaagct gcccctggcc cgcgtggtct acgccagcgc 1321 cacaggtgcc tctgagcctc ggaacatgat ctacatgagc cgcttgggta tctggggcga 1381 gggcacaccc ttccggaact ttgaggagtt cctgcacgcc atcgagaaga ggggcgttgg 1441 cgccatggag atcgtggcca tggacatgaa ggtcagcggc atgtacatcg cacgccagct 1501 cagcttctcc ggcgtcacct tccgcatcga ggagatcccg ctggccccag ccttcgagtg 1561 cgtctacaac cgcgcggccc tgctgtgggc cgaggccctg aacgtgttcc agcaggcggc 1621 cgactggatc ggcctggagt cgcgcaagtc cctgtggggc cagttctggt cggcacacca 1681 gcgcttcttc aagtatctgt gcatcgcagc caaggtgcgc cggctggtgg agctggcccg 1741 agaggagctg gcgcgagaca agtgcgtggt catcgggctg cagtccacgg gcgaggcgcg 1801 cacgcgggag gtgctggggg agaacgatgg gcacctcaac tgcttcgtct cggccgctga 1861 aggcgtgttc ctgtcgctaa ttcagaagca ctttccgtcc accaagagaa agcgggacag 1921 aggagcgggc agcaagcgga aacggcgacc tcggggacgc ggggccaaag ccccccggct 1981 ggcgtgcgag acagcgggcg tcatccgcat cagtgacgac agcagcacgg agtcggaccc 2041 tggcctggac agcgacttca actcctcccc cgagtccctg gtggatgacg acgttgtcat 2101 cgttgatgca gtcgggctcc ccagtgacga ccggggaccc ctgtgcctcc tgcagagaga 2161 cccgcatggc cccggggtcc tggagcgggt ggagcggctg aagcaggatc tgctggacaa 2221 agtgcggcgg ctgggccggg aactgccagt caacaccctg gacgagctca tcgaccagct 2281 gggcggcccc cagcgggtgg cggagatgac cggcaggaaa ggccgcgtgg tgtccaggcc 2341 cgacgggacg gtggccttcg agtcgcgggc agagcagggt ctgtccatcg accacgtgaa 2401 cctcagggag aagcagcgct tcatgagcgg cgagaagctc gtggccatca tctcggaggc 2461 ctccagctcg ggtgtctccc tccaagccga ccgccgtgtc cagaaccagc ggcgccgcgt 2521 gcacatgacc ttggagctgc cgtggagcgc cgaccgcgcc atccagcagt tcggccgcac 2581 ccaccggtcc aaccaggtct ccgcgccaga gtatgtcttc ctcatctcgg agctggccgg 2641 ggagcgccgg ttcgcctcca tcgtggccaa gcgcctggag agtctggggg ccctgaccca 2701 cggagaccgc cgcgccacgg agtcccgtga cctcagcaag tacaactttg agaacaagta 2761 tggcacccgg gccctgcact gtgtcctcac caccatcctg agccagactg agaacaaagt 2821 gcctgtgccc cagggatacc ctggaggggt ccccaccttc ttccgggaca tgaagcaggg 2881 cctgctgtct gtgggcattg gtggccggga gtcccggaat ggctgcctgg acgtggagaa 2941 ggactgttcc atcaccaagt tcctgaaccg catcctgggg ctggaggtgc acaagcagaa 3001 cgccctgttc cagtacttct cagacacctt cgaccacctc atcgagatgg acaagcggga 3061 gggcaaatac gacatgggca tcctggacct tgctcccggt atcgaggaga tctacgagga 3121 gagccagcag gtgttcctgg ctcccgggca cccgcaggac gggcaggtgg tcttctacaa 3181 gatcagcgtg gaccgcggcc tgaagtggga ggacgccttt gccaagtcgc tggcgctgac 3241 gggcccctat gacggcttct acctctccta caaggtccgc ggtaacaagc ccagctgcct 3301 gctggcggag cagaaccgcg gccagttctt cacggtgtac aagcccaaca tcggccggca 3361 gagccagctg gaggtcctgg acagcctccg ccgcaagttc caccgggtca ccgcggagga 3421 ggccaaggag ccctgggaga gtggctacgc tttgtcgctg acgcactgca gccacagcgc 3481 ctggaaccgg cactgccggc tggcgcagga gggtaaggac tgcctgcagg ggctgcggct 3541 gcggcaccac tacatgctgt gcggcgcgct gctgcgcgtg tggggccgca tcgccgccgt 3601 catggccgac gtcagcagca gcagctacct gcagatcgtg cggctgaaga ccaaggacag 3661 gaagaagcaa gtgggcatca agatccccga gggctgcgtg cgccgggtgc tgcaggagct 3721 gcggctgatg gatgcggacg tgaagcccag gcaggcgccc gccctgggct gccccgcccc 3781 gcccgccccg cgcccgctgg cgctgccttg cggccccgga gaggtgctgg acctcaccta 3841 cagccccccg gccgaggcct tcccgccgcc cccgcacttc tctttcccgg cgccgctgtc 3901 cctggacgcc ggccccGGCG TCGTGCCGCT GGGCACCCCC GACGCCCAGG CCGACCCTGC 3961 GGCCCTCGCG CACCAGGGCT GCGACATCAA CTTCAAGGAG GTGCTGGAGG ACATGCTGCG 4021 CTCGCTGCAC GCGGGGCCGC CCTCCGAGGG CGCGCTGGGG GAGGGCGCGG GGGCGGGGGG 4081 CGCGGCGGGC GGTGGTCCCG AGCGGCAGAG CGTGATCCAG TTCAGCCCAC CCTTCCCCGG 4141 CGCCCAGGCT CCTTTCTGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 4201 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 4261 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTTTC CTCGTCAAGT 4321 GAGTTTGAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt