Construct: ORF TRCN0000487974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021990.1_s317c1
- DNA Barcode:
- ACGCTTTTAGTTCCCAGCCAACGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLCO1B1 (10599)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10599 | SLCO1B1 | solute carrier organic anio... | NM_006446.5 | 99.9% | 99.8% | 388A>G |
| 2 | human | 28234 | SLCO1B3 | solute carrier organic anio... | NM_019844.4 | 85.6% | 78.3% | (many diffs) |
| 3 | human | 28234 | SLCO1B3 | solute carrier organic anio... | NM_001349920.2 | 82.1% | 75.3% | (many diffs) |
| 4 | human | 115072896 | SLCO1B3-SLCO1B7 | SLCO1B3-SLCO1B7 readthrough | NM_001371097.1 | 78.7% | 70.9% | (many diffs) |
| 5 | human | 338821 | SLCO1B7 | solute carrier organic anio... | NM_001009562.4 | 77.5% | 67.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2142
- ORF length:
- 2073
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaccaaaat caacatttga ataaaacagc agaggcacaa ccttcagaga 121 ataagaaaac aagatactgc aatggattga agatgttctt ggcagctctg tcactcagct 181 ttattgctaa gacactaggt gcaattatta tgaaaagttc catcattcat atagaacgga 241 gatttgagat atcctcttct cttgttggtt ttattgacgg aagctttgaa attggaaatt 301 tgcttgtgat tgtatttgtg agttactttg gatccaaact acatagacca aagttaattg 361 gaatcggttg tttcattatg ggaattggag gtgttttgac tgctttgcca catttcttca 421 tgggatatta caggtattct aaagaaacta atatcgattc atcagaaaat tcaacatcga 481 ccttatccac ttgtttaatt aatcaaattt tatcactcaa tagagcatca cctgagatag 541 tgggaaaagg ttgtttaaag gaatctgggt catacatgtg gatatatgtg ttcatgggta 601 atatgcttcg tggaataggg gagactccca tagtaccatt ggggctttct tacattgatg 661 atttcgctaa agaaggacat tcttctttgt atttaggtat attgaatgca atagcaatga 721 ttggtccaat cattggcttt accctgggat ctctgttttc taaaatgtac gtggatattg 781 gatatgtaga tctaagcact atcaggataa ctcctactga ttctcgatgg gttggagctt 841 ggtggcttaa tttccttgtg tctggactat tctccattat ttcttccata ccattctttt 901 tcttgcccca aactccaaat aaaccacaaa aagaaagaaa agcttcactg tctttgcatg 961 tgctggaaac aaatgatgaa aaggatcaaa cagctaattt gaccaatcaa ggaaaaaata 1021 ttaccaaaaa tgtgactggt tttttccagt cttttaaaag catccttact aatcccctgt 1081 atgttatgtt tgtgcttttg acgttgttac aagtaagcag ctatattggt gcttttactt 1141 atgtcttcaa atacgtagag caacagtatg gtcagccttc atctaaggct aacatcttat 1201 tgggagtcat aaccatacct atttttgcaa gtggaatgtt tttaggagga tatatcatta 1261 aaaaattcaa actgaacacc gttggaattg ccaaattctc atgttttact gctgtgatgt 1321 cattgtcctt ttacctatta tattttttca tactctgtga aaacaaatca gttgccggac 1381 taaccatgac ctatgatgga aataatccag tgacatctca tagagatgta ccactttctt 1441 attgcaactc agactgcaat tgtgatgaaa gtcaatggga accagtctgt ggaaacaatg 1501 gaataactta catctcaccc tgtctagcag gttgcaaatc ttcaagtggc aataaaaagc 1561 ctatagtgtt ttacaactgc agttgtttgg aagtaactgg tctccagaac agaaattact 1621 cagcccattt gggtgaatgc ccaagagatg atgcttgtac aaggaaattt tacttttttg 1681 ttgcaataca agtcttgaat ttatttttct ctgcacttgg aggcacctca catgtcatgc 1741 tgattgttaa aattgttcaa cctgaattga aatcacttgc actgggtttc cactcaatgg 1801 ttatacgagc actaggagga attctagctc caatatattt tggggctcTG ATTGATACAA 1861 CGTGTATAAA GTGGTCCACC AACAACTGTG GCACACGTGG GTCATGTAGG ACATATAATT 1921 CCACATCATT TTCAAGGGTC TACTTGGGCT TGTCTTCAAT GTTAAGAGTC TCATCACTTG 1981 TTTTATATAT TATATTAATT TATGCCATGA AGAAAAAATA TCAAGAGAAA GATATCAATG 2041 CATCAGAAAA TGGAAGTGTC ATGGATGAAG CAAACTTAGA ATCCTTAAAT AAAAATAAAC 2101 ATTTTGTCCC TTCTGCTGGG GCAGATAGTG AAACACATTG TTAAGACCCA GCTTTCTTGT 2161 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2221 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2281 GAAAGGACGA ACGCTTTTAG TTCCCAGCCA ACGCACGCGT TAAGTCgaca atcaacctct 2341 ggattacaaa atttgtgaaa gatt