Construct: ORF TRCN0000487995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021474.1_s317c1
- DNA Barcode:
- GTGCCGGCTTGGTTGTACACCGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A3 (6531)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487995
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6531 | SLC6A3 | solute carrier family 6 mem... | NM_001044.5 | 99.9% | 100% | 1467C>A |
| 2 | mouse | 13162 | Slc6a3 | solute carrier family 6 (ne... | NM_010020.3 | 87.3% | 93.5% | (many diffs) |
| 3 | mouse | 13162 | Slc6a3 | solute carrier family 6 (ne... | XM_006517083.3 | 81% | 86.6% | (many diffs) |
| 4 | mouse | 13162 | Slc6a3 | solute carrier family 6 (ne... | XM_017315377.1 | 74.6% | 80.4% | (many diffs) |
| 5 | mouse | 13162 | Slc6a3 | solute carrier family 6 (ne... | XM_006517084.3 | 57.9% | 57.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 78
- ORF end:
- 1938
- ORF length:
- 1860
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcga attcaccatg agtaagagca aatgctccgt gggactcatg tcttccgtgg 121 tggccccggc taaggagccc aatgccgtgg gcccgaagga ggtggagctc atccttgtca 181 aggagcagaa cggagtgcag ctcaccagct ccaccctcac caacccgcgg cagagccccg 241 tggaggccca ggatcgggag acctggggca agaagatcga ctttctcctg tccgtcattg 301 gctttgctgt ggacctggcc aacgtctggc ggttccccta cctgtgctac aaaaatggtg 361 gcggtgcctt cctggtcccc tacctgctct tcatggtcat tgctgggatg ccacttttct 421 acatggagct ggccctcggc cagttcaaca gggaaggggc cgctggtgtc tggaagatct 481 gccccatact gaaaggtgtg ggcttcacgg tcatcctcat ctcactgtat gtcggcttct 541 tctacaacgt catcatcgcc tgggcgctgc actatctctt ctcctccttc accacggagc 601 tcccctggat ccactgcaac aactcctgga acagccccaa ctgctcggat gcccatcctg 661 gtgactccag tggagacagc tcgggcctca acgacacttt tgggaccaca cctgctgccg 721 agtactttga acgtggcgtg ctgcacctcc accagagcca tggcatcgac gacctggggc 781 ctccgcggtg gcagctcaca gcctgcctgg tgctggtcat cgtgctgctc tacttcagcc 841 tctggaaggg cgtgaagacc tcagggaagg tggtatggat cacagccacc atgccatacg 901 tggtcctcac tgccctgctc ctgcgtgggg tcaccctccc tggagccata gacggcatca 961 gagcatacct gagcgttgac ttctaccggc tctgcgaggc gtctgtttgg attgacgcgg 1021 ccacccaggt gtgcttctcc ctgggcgtgg ggttcggggt gctgatcgcc ttctccagct 1081 acaacaagtt caccaacaac tgctacaggg acgcgattgt caccacctcc atcaactccc 1141 tgacgagctt ctcctccggc ttcgtcgtct tctccttcct ggggtacatg gcacagaagc 1201 acagtgtgcc catcggggac gtggccaagg acgggccagg gctgatcttc atcatctacc 1261 cggaagccat cgccacgctc cctctgtcct cagcctgggc cgtggtcttc ttcatcatgc 1321 tgctcaccct gggtatcgac agcgccatgg gtggtatgga gtcagtgatc accgggctca 1381 tcgatgagtt ccagctgctg cacagacacc gtgagctctt cacgctcttc atcgtcctgg 1441 cgaccttcct cctgtccctg ttctgcgtca ccaacggtgg catctacgtc ttcacgctcc 1501 tggaccattt tgcagccggc acgtccatcc tctttggagt gctaatcgaa gccatcggag 1561 tggcctggtt ctatggtgtt gggCAGTTCA GCGACGACAT CCAGCAGATG ACCGGGCAGC 1621 GGCCCAGCCT GTACTGGCGG CTGTGCTGGA AGCTGGTCAG CCCCTGCTTT CTCCTGTTCG 1681 TGGTCGTGGT CAGCATTGTG ACCTTCAGAC CCCCCCACTA CGGAGCCTAC ATCTTCCCCG 1741 ACTGGGCCAA CGCGCTGGGC TGGGTCATCG CCACATCCTC CATGGCCATG GTGCCCATCT 1801 ATGCGGCCTA CAAGTTCTGC AGCCTGCCTG GGTCCTTTCG AGAGAAACTG GCCTACGCCA 1861 TTGCACCCGA GAAGGACCGT GAGCTGGTGG ACAGAGGGGA GGTGCGCCAG TTCACGCTCC 1921 GCCACTGGCT CAAGGTGTAG AAGCTTGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1981 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2041 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTGCCGGC 2101 TTGGTTGTAC ACCGCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2161 aagatt