Construct: ORF TRCN0000488003
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019919.1_s317c1
- DNA Barcode:
- TTTTCGTCCCAAATCAATGTTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIPE (3991)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488003
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3991 | LIPE | lipase E, hormone sensitive... | NM_005357.4 | 99.9% | 99.9% | 3228_3229insG |
| 2 | human | 3991 | LIPE | lipase E, hormone sensitive... | XM_005258937.3 | 92.9% | 92.8% | 2135_2136ins228;3000_3001insG |
| 3 | human | 3991 | LIPE | lipase E, hormone sensitive... | XM_005258939.3 | 74.7% | 71.3% | (many diffs) |
| 4 | human | 3991 | LIPE | lipase E, hormone sensitive... | XM_005258938.5 | 74.3% | 72.4% | (many diffs) |
| 5 | human | 3991 | LIPE | lipase E, hormone sensitive... | XM_005258940.3 | 72% | 71.9% | 0_1ins903;2325_2326insG |
| 6 | human | 3991 | LIPE | lipase E, hormone sensitive... | XM_006723218.3 | 72% | 71.9% | 0_1ins903;2325_2326insG |
| 7 | human | 3991 | LIPE | lipase E, hormone sensitive... | XM_017026810.1 | 72% | 71.9% | 0_1ins903;2325_2326insG |
| 8 | human | 3991 | LIPE | lipase E, hormone sensitive... | XM_024451514.1 | 67.3% | 65.4% | (many diffs) |
| 9 | mouse | 16890 | Lipe | lipase, hormone sensitive | NM_001039507.2 | 57.9% | 59.8% | (many diffs) |
| 10 | mouse | 16890 | Lipe | lipase, hormone sensitive | XM_006539571.2 | 57.9% | 59.8% | (many diffs) |
| 11 | mouse | 16890 | Lipe | lipase, hormone sensitive | XM_006539572.3 | 57.9% | 59.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3300
- ORF length:
- 3231
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagccaggt tctaagtcag tgtctaggtc agactggcaa cctgaaccac 121 accagaggcc tataaccccg ctagagcctg ggccagaaaa gacacccata gcccagccag 181 aatcgaagac tctgcaggga tccaataccc aacagaagcc tgcttcaaac caaagacccc 241 tcacccagca ggagacccct gcacaacatg atgctgaatc ccagaaggaa cctagagccc 301 aacaaaaatc tgcttcacaa gaggaatttc ttgccccaca gaagcccgca ccacagcaat 361 caccttacat ccaaagggtg ctgctcactc aacaggaagc tgcctcccag cagggacctg 421 ggctaggaaa agaatctata actcaacagg agccagcatt gagacaaaga catgtagccc 481 agccagggcc tgggccagga gagccacctc cagctcaaca agaagctgaa tcaacacctg 541 cggcccaggc taaacctgga gccaaaaggg agccatctgc cccgactgaa tctacgtccc 601 aagagacacc tgaacagtca gacaagcaaa caacgccagt ccagggagcc aaatccaagc 661 agggatcttt gacagagctg ggatttctaa caaaacttca ggaactatcc atacagcgat 721 cagccctaga gtggaaggca ctttctgagt gggtcacaga ttctgagtca gaatcagatg 781 tgggatcatc ttcagacaca gattctccag ccacgatggg tggaatggtg gcccagggag 841 tgaagctagg cttcaaagga aaatctggtt ataaagtgat gtcaggatac agtgggacgt 901 cgccacatga gaaaaccagt gctcggaatc acagacacta ccaggataca gcctcaaggc 961 tcatccacaa catggacctg cgcacaatga cacagtcgct ggtgactctg gcggaggaca 1021 acatagcctt cttctcgagc cagggtcctg gggaaacggc ccagcggctg tcaggcgttt 1081 ttgccggtgt acgggagcag gcgctggggc tggagccggc cctgggccgc ctgctgggtg 1141 tggcgcacct ctttgacctg gacccagaga caccggccaa cgggtaccgc agcctagtgc 1201 acacagcccg ctgctgcctg gcgcacctcc tgcacaaatc ccgctatgtg gcctccaacc 1261 gccgcagcat cttcttccgc accagccaca acctggccga gctggaggcc tacctggctg 1321 ccctcaccca gctccgcgct ctggtctact acgcccagcg cctgctggtt accaatcggc 1381 cgggggtact cttctttgag ggcgacgagg ggctcaccgc cgacttcctc cgggagtatg 1441 tcacgctgca taagggatgc ttctatggcc gctgcctggg cttccagttc acgcctgcca 1501 tccggccatt cctgcagacc atctccattg ggctggtgtc cttcggggag cactacaaac 1561 gcaacgagac aggcctcagt gtggccgcca gctctctctt caccagcggc cgctttgcca 1621 tcgaccccga gctgcgtggg gctgagtttg agcggatcac acagaacctg gacgtgcact 1681 tctggaaagc cttctggaac atcaccgaga tggaagtgct atcgtctctg gccaacatgg 1741 catcggccac cgtgagggta agccgcctgc tcagcctgcc acccgaagcc tttgagatgc 1801 cactgactgc cgaccccacg ctcacggtca ccatctcacc cccactggcc cacacaggcc 1861 ctgggcccgt cctcgtcagg ctcatctcct atgacctgcg tgaaggacag gacagtgagg 1921 agctcagcag cctgataaag tccaacggcc aacggagcct ggagctgtgg ccgcgccccc 1981 agcaggcacc ccgctcgcgg tccctgatag tgcacttcca cggcggtggc tttgtggccc 2041 agacctccag atcccacgag ccctacctca agagctgggc ccaggagctg ggcgccccca 2101 tcatctccat cgactactcc ctggcccctg aggccccctt cccccgtgcg ctggaggagt 2161 gcttcttcgc ctactgctgg gccatcaagc actgcgccct ccttggctca acaggggaac 2221 gaatctgcct tgcgggggac agtgcaggcg ggaacctctg cttcaccgtg gctcttcggg 2281 cagcagccta cggggtgcgg gtgccagatg gcatcatggc agcctacccg gccacaatgc 2341 tgcagcctgc cgcctctccc tcccgcctgc tgagcctcat ggaccccttg ctgcccctca 2401 gtgtgctctc caagtgtgtc agcgcctatg ctggtgcaaa gacggaggac cactccaact 2461 cagaccagaa agccctcggc atgatggggc tggtgcggcg ggacacagcc ctgctcctcc 2521 gagacttccg cctgggtgcc tcctcatggc tcaactcctt cctggagtta agtgggcgca 2581 agtcccagaa gatgtcggag cccatagcag agccgatgcg ccgcagtgtg tctgaagcag 2641 cactggccca gccccagggc ccactgggca cggattccct caagaacctg accctgaggg 2701 acttgagcct gaggggaaac tccgagacgt cgtcggacac ccccgagatg tcgctgtcag 2761 ctgagacact tagcccctcc acaccctccg atgtcaactt cttattacca cctgaggatg 2821 caggggaaga ggctgaggcc aaaaatgagc tgagccccat ggacagaggc ctgggcgtcc 2881 gtgccgcctt ccccgagggt ttccaccccc gacgctccag ccagggtgcc acacagatgc 2941 ccctctactc ctcacccata gtcaagaacc ccttcatgtc gccgctgctg gcacccgaca 3001 gcatgctcaa gagcctgcca cctgtgcaca tcgtggcgtg cgcgctggac cccatgctgg 3061 acgactcggt catgctcgcg cggcgactgc gcaacctggg ccagccggtg acgctgcgcg 3121 tggtggagga cctgccgcac ggcttcctga ccctagcggc gctgtgccgc gagacgcgcc 3181 aggccgcaga gctgtgcgtg gagcgcatcc gcctcgtcct cactcctccc gccggagccg 3241 ggccgagcgg ggagacgggg gctgcggggg taGACGGGGG CTGCGGGGGG CGACACGACC 3301 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3361 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3421 ATATATCTTG TGGAAAGGAC GATTTTCGTC CCAAATCAAT GTTCCCACGC GTTAAGTCga 3481 caatcaacct ctggattaca aaatttgtga aagatt