Construct: ORF TRCN0000488010
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020509.1_s317c1
- DNA Barcode:
- CTAAGCAGGACAGCTGATGCTCGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SIK2 (23235)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488010
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23235 | SIK2 | salt inducible kinase 2 | NM_015191.3 | 99.7% | 99.7% | 1_6delATGGTC |
| 2 | human | 23235 | SIK2 | salt inducible kinase 2 | XM_017017417.1 | 92.6% | 92.6% | 0_1ins204 |
| 3 | human | 23235 | SIK2 | salt inducible kinase 2 | XM_017017418.1 | 83.6% | 83.6% | 0_1ins453 |
| 4 | mouse | 235344 | Sik2 | salt inducible kinase 2 | NM_178710.3 | 88.1% | 90.5% | (many diffs) |
| 5 | mouse | 235344 | Sik2 | salt inducible kinase 2 | XM_006510266.1 | 88.1% | 90.6% | (many diffs) |
| 6 | mouse | 235344 | Sik2 | salt inducible kinase 2 | XM_006510267.2 | 85.7% | 88.3% | (many diffs) |
| 7 | mouse | 235344 | Sik2 | salt inducible kinase 2 | XM_017313332.1 | 85.7% | 88.4% | (many diffs) |
| 8 | mouse | 235344 | Sik2 | salt inducible kinase 2 | XR_379116.1 | 36.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2844
- ORF length:
- 2772
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggat ggcccgaggc acttgcagcg cgggccggtc cgggtggggt 121 tctacgacat cgagggcacg ctgggcaagg gcaacttcgc tgtggtgaag ctggggcggc 181 accggatcac caagacggag gtggcaataa aaataatcga taagtctcag ctggatgcag 241 tgaaccttga gaaaatctac cgagaagtac aaataatgaa aatgttagac caccctcaca 301 taatcaaact ttatcaggta atggagacca aaagtatgtt gtaccttgtg acagaatatg 361 ccaaaaatgg agaaattttt gactatcttg ctaatcatgg ccggttaaat gagtctgaag 421 ccaggcgaaa attctggcaa atcctgtctg ctgttgatta ttgtcatggt cggaagattg 481 tgcaccgtga cctcaaagct gaaaatctcc tgctggataa caacatgaat atcaaaatag 541 cagatttcgg ttttggaaat ttctttaaaa gtggtgaact gctggcaaca tggtgtggca 601 gcccccctta tgcagcccca gaagtctttg aagggcagca gtatgaagga ccacagctgg 661 acatctggag tatgggagtt gttctttatg tccttgtctg tggagctctg ccctttgatg 721 gaccgactct tccaattttg aggcagaggg ttctggaagg aagattccgg attccgtatt 781 tcatgtcaga agattgcgag caccttatcc gaaggatgtt ggtcctagac ccatccaaac 841 ggctaaccat agcccaaatc aaggagcata aatggatgct catagaagtt cctgtccaga 901 gacctgttct ctatccacaa gagcaagaaa atgagccatc catcggggag tttaatgagc 961 aggttctgcg actgatgcac agccttggaa tagatcagca gaaaaccatt gagtctttgc 1021 agaacaagag ctataaccac tttgctgcca tttatttctt gttggtggag cgcctgaaat 1081 cacatcggag cagtttccca gtggagcaga gacttgatgg ccgccagcgt cggcctagca 1141 ccattgctga gcaaacagtt gccaaggcac agactgtggg gctcccagtg accatgcatt 1201 caccgaacat gaggctgctg cgatctgccc tcctccccca ggcatccaac gtggaggcct 1261 tttcatttcc agcatctggc tgtcaggcgg aagctgcatt catggaagaa gagtgtgtgg 1321 acactccaaa ggtcaatggc tgtctgcttg accctgtgcc tcctgtcctg gtgcggaagg 1381 gatgccagtc actgcccagc aacatgatgg agacctccat tgacgaaggg ctggagacag 1441 aaggagaggc cgaggaagac cccgctcatg cctttgaggc atttcagtcc acacgcagcg 1501 ggcagagacg gcacactctg tcagaagtga ccaatcaact ggtcgtgatg cctggggcag 1561 ggaaaatttt ctccatgaat gacagcccct cccttgacag tgtggactct gagtatgata 1621 tggggtctgt tcagagggac ctgaactttc tggaagacaa cccttccctt aaggacatca 1681 tgttagccaa tcagccttca ccccgcatga catctccctt cataagcctg agacctacca 1741 acccagccat gcaggctctg agctcccaga aacgagaggt ccacaacagg tctccagtga 1801 gcttcagaga gggccgcaga gcatcagata cctccctcac ccagggaatt gtagcattta 1861 gacaacatct tcagaatctg gctagaacca aaggaattct agagttgaac aaagtgcagt 1921 tgttgtatga acaaatagga ccggaggcag accctaacct ggcgccggcg gctcctcagc 1981 tccaggacct tgctagcagc tgccctcagg aagaagtttc tcagcagcag gaaagcgtct 2041 ccactctccc tgccagcgtg catccccagc tgtccccacg gcagagcctg gagacccagt 2101 acctgcagca cagactccag aagcccagcc ttctgtcaaa ggcccagaac acctgtcagc 2161 tttattgcaa agaaccaccg cggagccttg agcagcagct gcaggaacat aggctccagc 2221 agaagcgact ctttcttcag aagcagtctc aactgcaggc ctattttaat cagatgcaga 2281 tagcagagag ctcctaccca cagccaagtc agcagctgcc ccttccccgc caggagactc 2341 caccgccttc tcagcaggcc ccaccgttca gcctgaccca gcccctgagc cccgtcctgg 2401 agccttcctc cgagcagatg caatacagcc ctttcctcag ccagtaccaa gagatgcagc 2461 ttcagcccct gccctccact tccggtcccc gggctgctcc tcctctgccc acgcagctac 2521 agcagcagca gccgccaccg ccaccacccc cTCCACCACC ACGACAGCCA GGAGCTGCCC 2581 CAGCCCCCTT ACAGTTCTCC TATCAGACTT GTGAGCTGCC AAGCGCTGCT TCCCCTGCGC 2641 CAGACTATCC CACTCCCTGT CAGTATCCTG TGGATGGAGC CCAGCAGAGC GACCTAACGG 2701 GGCCAGACTG TCCCAGAAGC CCAGGACTGC AAGAGGCCCC CTCCAGCTAC GACCCACTAG 2761 CCCTCTCTGA GCTACCTGGA CTCTTTGATT GTGAAATGCT AGACGCTGTG GATCCACAAC 2821 ACAACGGGTA TGTCCTGGTG AATTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 2881 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2941 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTAAGCAG 3001 GACAGCTGAT GCTCGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3061 aagatt