Construct: ORF TRCN0000488017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021381.1_s317c1
- DNA Barcode:
- GCCGAGTCGAGGCCTAAATCATTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADORA2A (135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135 | ADORA2A | adenosine A2a receptor | NM_000675.6 | 99.9% | 100% | 1083T>C |
| 2 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278497.1 | 99.9% | 100% | 1083T>C |
| 3 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278498.1 | 99.9% | 100% | 1083T>C |
| 4 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278499.2 | 99.9% | 100% | 1083T>C |
| 5 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278500.1 | 99.9% | 100% | 1083T>C |
| 6 | human | 135 | ADORA2A | adenosine A2a receptor | NR_103543.2 | 47.5% | (many diffs) | |
| 7 | human | 135 | ADORA2A | adenosine A2a receptor | NR_103544.2 | 47.4% | (many diffs) | |
| 8 | human | 101730217 | SPECC1L-ADORA2A | SPECC1L-ADORA2A readthrough... | NR_103546.1 | 19.6% | 1_4179del;5262T>C;5416_6289del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1305
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcccatcatg ggctcctcgg tgtacatcac ggtggagctg gccattgctg 121 tgctggccat cctgggcaat gtgctggtgt gctgggccgt gtggctcaac agcaacctgc 181 agaacgtcac caactacttt gtggtgtcac tggcggcggc cgacatcgca gtgggtgtgc 241 tcgccatccc ctttgccatc accatcagca ccgggttctg cgctgcctgc cacggctgcc 301 tcttcattgc ctgcttcgtc ctggtcctca cgcagagctc catcttcagt ctcctggcca 361 tcgccattga ccgctacatt gccatccgca tcccgctccg gtacaatggc ttggtgaccg 421 gcacgagggc taagggcatc attgccatct gctgggtgct gtcgtttgcc atcggcctga 481 ctcccatgct aggttggaac aactgcggtc agccaaagga gggcaagaac cactcccagg 541 gctgcgggga gggccaagtg gcctgtctct ttgaggatgt ggtccccatg aactacatgg 601 tgtacttcaa cttctttgcc tgtgtgctgg tgcccctgct gctcatgctg ggtgtctatt 661 tgcggatctt cctggcggcg cgacgacagc tgaagcagat ggagagccag cctctgccgg 721 gggagcgggc acggtccaca ctgcagaagg aggtccatgc tgccaagtca ctggccatca 781 ttgtggggct ctttgccctc tgctggctgc ccctacacat catcaactgc ttcactttct 841 tctgccccga ctgcagccac gcccctctct ggctcatgta cctggccatc gtcctctccc 901 acaccaattc ggttgtgaat cccttcatct acgcctaccg tatccgcgag ttccgccaga 961 ccttccgcaa gatcattcgc agccacgtcc tgaggcagca agaaCCTTTC AAGGCAGCTG 1021 GCACCAGTGC CCGGGTCTTG GCAGCTCATG GCAGTGACGG AGAGCAGGTC AGCCTCCGTC 1081 TCAACGGCCA CCCGCCAGGA GTGTGGGCCA ACGGCAGTGC TCCCCACCCT GAGCGGAGGC 1141 CCAATGGCTA CGCCCTGGGG CTGGTGAGTG GAGGGAGTGC CCAAGAGTCC CAGGGGAACA 1201 CGGGCCTCCC AGACGTGGAG CTCCTTAGCC ATGAGCTCAA GGGAGTGTGC CCAGAGCCCC 1261 CTGGCCTAGA TGACCCCCTG GCCCAGGATG GAGCAGGAGT GTCCTAGAAC CCAGCTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGAGCCGAGT CGAGGCCTAA ATCATTAACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt