Construct: ORF TRCN0000488022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020287.1_s317c1
- DNA Barcode:
- GTCCCAGGCGTGAGGACTCCACGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BTK (695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488022
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 695 | BTK | Bruton tyrosine kinase | NM_000061.2 | 99.8% | 99.8% | 1977_1978insTTG |
| 2 | human | 695 | BTK | Bruton tyrosine kinase | NM_001287344.1 | 94.9% | 94.9% | 1_102del;2079_2080insTTG |
| 3 | human | 695 | BTK | Bruton tyrosine kinase | NM_001287345.1 | 73.1% | 73.1% | 1033_1034ins528;1449_1450insTTG |
| 4 | mouse | 12229 | Btk | Bruton agammaglobulinemia t... | NM_013482.2 | 91.3% | 98.1% | (many diffs) |
| 5 | mouse | 12229 | Btk | Bruton agammaglobulinemia t... | XM_006528483.3 | 91.3% | 98.1% | (many diffs) |
| 6 | mouse | 12229 | Btk | Bruton agammaglobulinemia t... | XM_006528484.3 | 91.3% | 98.1% | (many diffs) |
| 7 | mouse | 12229 | Btk | Bruton agammaglobulinemia t... | XM_006528485.3 | 91.3% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2052
- ORF length:
- 1980
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgca gtgattctgg agagcatctt tctgaagcga tcccaacaga 121 aaaagaaaac atcacctcta aacttcaaga agcgcctgtt tctcttgacc gtgcacaaac 181 tctcctacta tgagtatgac tttgaacgtg ggagaagagg cagtaagaag ggttcaatag 241 atgttgagaa gatcacttgt gttgaaacag tggttcctga aaaaaatcct cctccagaaa 301 gacagattcc gagaagaggt gaagagtcca gtgaaatgga gcaaatttca atcattgaaa 361 ggttccctta tcccttccag gttgtatatg atgaagggcc tctctacgtc ttctccccaa 421 ctgaagaact aaggaagcgg tggattcacc agctcaaaaa cgtaatccgg tacaacagtg 481 atctggttca gaaatatcac ccttgcttct ggatcgatgg gcagtatctc tgctgctctc 541 agacagccaa aaatgctatg ggctgccaaa ttttggagaa caggaatgga agcttaaaac 601 ctgggagttc tcaccggaag acaaaaaagc ctcttccccc aacgcctgag gaggaccaga 661 tcttgaaaaa gccactaccg cctgagccag cagcagcacc agtctccaca agtgagctga 721 aaaaggttgt ggccctttat gattacatgc caatgaatgc aaatgatcta cagctgcgga 781 agggtgatga atattttatc ttggaggaaa gcaacttacc atggtggaga gcacgagata 841 aaaatgggca ggaaggctac attcctagta actatgtcac tgaagcagaa gactccatag 901 aaatgtatga gtggtattcc aaacacatga ctcggagtca ggctgagcaa ctgctaaagc 961 aagaggggaa agaaggaggt ttcattgtca gagactccag caaagctggc aaatatacag 1021 tgtctgtgtt tgctaaatcc acaggggacc ctcaaggggt gatacgtcat tatgttgtgt 1081 gttccacacc tcagagccag tattacctgg ctgagaagca ccttttcagc accatccctg 1141 agctcattaa ctaccatcag cacaactctg caggactcat atccaggctc aaatatccag 1201 tgtctcaaca aaacaagaat gcaccttcca ctgcaggcct gggatacgga tcatgggaaa 1261 ttgatccaaa ggacctgacc ttcttgaagg agctggggac tggacaattt ggggtagtga 1321 agtatgggaa atggagaggc cagtacgacg tggccatcaa gatgatcaaa gaaggctcca 1381 tgtctgaaga tgaattcatt gaagaagcca aagtcatgat gaatctttcc catgagaagc 1441 tggtgcagtt gtatggcgtc tgcaccaagc agcgccccat cttcatcatc actgagtaca 1501 tggccaatgg ctgcctcctg aactacctga gggagatgcg ccaccgcttc cagactcagc 1561 agctgctaga gatgtgcaag gatgtctgtg aagccatgga atacctggag tcaaagcagt 1621 tccttcaccg agacctggca gctcgaaact gtttggtaaa cgatcaagga gttgttaaag 1681 tatctgattt cggcctgtcc aggtatgtcc tggatgatga atacacaagc tcagtaggct 1741 ccaaatttcc agtccggtgg tccccaccgg aagtccTGAT GTATAGCAAG TTCAGCAGCA 1801 AATCTGACAT TTGGGCTTTT GGGGTTTTGA TGTGGGAAAT TTACTCCCTG GGGAAGATGC 1861 CATATGAGAG ATTTACTAAC AGTGAGACTG CTGAACACAT TGCCCAAGGC CTACGTCTCT 1921 ACAGGCCTCA TCTGGCTTCA GAGAAGGTAT ATACCATCAT GTACAGTTGC TGGCATGAGA 1981 AAGCAGATGA GCGTCCCACT TTCAAAATTC TTCTGAGCAA TATTCTAGAT GTCATGGATG 2041 AAGAATCCTT GTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2101 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2161 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTCCCAGGCG TGAGGACTCC 2221 ACGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt