Construct: ORF TRCN0000488032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021095.1_s317c1
- DNA Barcode:
- AGTCTGTGCCGTGAGGCCCTTAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KISS1R (84634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488032
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84634 | KISS1R | KISS1 receptor | NM_032551.5 | 100% | 100% | |
| 2 | human | 84634 | KISS1R | KISS1 receptor | XM_017027382.1 | 56.7% | 44% | 503_504ins233;678_679ins283 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1266
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcacacc gtggctacgt ccggacccaa cgcgtcctgg ggggcaccgg 121 ccaacgcctc cggctgcccg ggctgtggcg ccaacgcctc ggacggccca gtcccttcgc 181 cgcgggccgt ggacgcctgg ctcgtgccgc tcttcttcgc ggcgctgatg ctgctgggcc 241 tggtggggaa ctcgctggtc atctacgtca tctgccgcca caagccgatg cggaccgtga 301 ccaacttcta catcgccaac ctggcggcca cggacgtgac cttcctcctg tgctgcgtcc 361 ccttcacggc cctgctgtac ccgctgcccg gctgggtgct gggcgacttc atgtgcaagt 421 tcgtcaacta catccagcag gtctcggtgc aggccacgtg tgccactctg accgccatga 481 gtgtggaccg ctggtacgtg acggtgttcc cgttgcgcgc cctgcaccgc cgcacgcccc 541 gcctggcgct ggctgtcagc ctcagcatct gggtaggctc tgcggcggtg tctgcgccgg 601 tgctcgccct gcaccgcctg tcacccgggc cgcgcgccta ctgcagtgag gccttcccca 661 gccgcgccct ggagcgcgcc ttcgcactgt acaacctgct ggcgctgtac ctgctgccgc 721 tgctcgccac ctgcgcctgc tatgcggcca tgctgcgcca cctgggccgg gtcgccgtgc 781 gccccgcgcc cgccgatagc gccctgcagg ggcaggtgct ggcagagcgc gcaggcgccg 841 tgcgggccaa ggtctcgcgg ctggtggcgg ccgtggtcct gctcttcgcc gcctgctggg 901 gccccatcca gctgttcctg gtgctgcagg cgctgggccc cgcgggctcc tggcacccac 961 gcagctacgc cgccTACGCG CTTAAGACCT GGGCTCACTG CATGTCCTAC AGCAACTCCG 1021 CGCTGAACCC GCTGCTCTAC GCCTTCCTGG GCTCGCACTT CCGACAGGCC TTCCGCCGCG 1081 TCTGCCCCTG CGCGCCGCGC CGCCCCCGCC GCCCCCGCCG GCCCGGACCC TCGGACCCCG 1141 CAGCCCCACA CGCGGAGCTG CTCCGCCTGG GGTCCCACCC GGCCCCCGCC AGGGCGCAGA 1201 AGCCAGGGAG CAGTGGGCTG GCCGCGCGCG GGCTGTGCGT CCTGGGGGAG GACAACGCCC 1261 CTCTCTGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGTCTG TGCCGTGAGG CCCTTAAAAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt