Construct: ORF TRCN0000488040
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020198.1_s317c1
- DNA Barcode:
- TTCCGTGCTCTCTAGCCAAACACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ITPK1 (3705)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488040
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | NM_001142593.3 | 99.9% | 100% | 876C>T |
| 2 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | NM_014216.6 | 99.9% | 100% | 876C>T |
| 3 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | XM_017021262.1 | 82.3% | 80% | (many diffs) |
| 4 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | NM_001142594.3 | 73.8% | 72.6% | (many diffs) |
| 5 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | NM_001363707.1 | 71.1% | 71.2% | 0_1ins357;519C>T |
| 6 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | XM_017021263.1 | 68.5% | 68.5% | 0_1ins390;486C>T |
| 7 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | XM_017021264.1 | 68.5% | 68.5% | 0_1ins390;486C>T |
| 8 | mouse | 217837 | Itpk1 | inositol 1,3,4-triphosphate... | NM_172584.3 | 87.2% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1314
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagacc tttctgaaag ggaagagagt tggctactgg ctgagcgaga 121 agaaaatcaa gaagctgaat ttccaggcct tcgccgagct gtgcaggaag cgagggatgg 181 aggttgtgca gctgaacctt agccggccga tcgaggagca gggccccctg gacgtcatca 241 tccacaagct gactgacgtc atccttgaag ccgaccagaa tgatagccag tccctggagc 301 tggtgcacag gttccaggag tacatcgatg cccaccctga gaccatcgtc ctggacccgc 361 tccctgccat cagaaccctg cttgaccgct ccaagtccta tgagctcatc cggaagattg 421 aggcctacat ggaagacgac aggatctgct cgccaccctt catggagctc acgagcctgt 481 gcggggatga caccatgcgg ctgctggaga agaacggctt gactttccca ttcatttgca 541 aaaccagagt ggctcatggc accaactctc acgagatggc tatcgtgttc aaccaggagg 601 gcctgaacgc catccagcca ccctgcgtgg tccagaattt catcaaccac aacgccgtcc 661 tgtacaaggt gttcgtggtt ggcgagtcct acaccgtggt ccagaggccc tcactcaaga 721 acttctccgc aggcacatca gaccgtgagt ccatcttctt caacagccac aacgtgtcaa 781 agccggagtc gtcatcggtc ctgacggagc tggacaagat cgagggcgtg ttcgagcggc 841 cgagcgacga ggtcatccgg gagctctccc gggccctgcg gcaggcactg ggcgtgtcac 901 tcttcggcat cgacatcatc atcaacaacc agacagggca gcacgctgtc attgacatca 961 atgccttccc aggctacgag ggcgtgagcg agttcttcac agacctcctg aaccacatcg 1021 ccactgtcct gcagggccag agcacagcca tggcagccac aggggacgtg gccctgctga 1081 ggcacagcaa gcttctggcc gagccggcgg gcggcctgGT GGGCGAGCGG ACATGCAGCG 1141 CCAGCCCCGG CTGCTGCGGC AGCATGATGG GCCAGGACGC GCCCTGGAAG GCTGAGGCCG 1201 ACGCGGGCGG CACCGCCAAG CTGCCGCACC AGAGACTCGG CTGCAACGCC GGCGTGTCGC 1261 CCAGCTTCCA GCAGCATTGT GTGGCCTCCC TGGCCACCAA GGCCTCCTCC CAGTGAGACC 1321 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GATTCCGTGC TCTCTAGCCA AACACTACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt