Construct: ORF TRCN0000488042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020045.1_s317c1
- DNA Barcode:
- CTCGTACGGCGAGAGTTATAGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRM6 (2916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2916 | GRM6 | glutamate metabotropic rece... | NM_000843.4 | 99.9% | 99.8% | 2631_2632insG |
| 2 | mouse | 108072 | Grm6 | glutamate receptor, metabot... | NM_173372.2 | 87.2% | 93.1% | (many diffs) |
| 3 | mouse | 108072 | Grm6 | glutamate receptor, metabot... | XM_006531961.2 | 87.2% | 93.1% | (many diffs) |
| 4 | mouse | 108072 | Grm6 | glutamate receptor, metabot... | XM_017314218.1 | 52.3% | 56.6% | (many diffs) |
| 5 | mouse | 108072 | Grm6 | glutamate receptor, metabot... | XM_006531964.1 | 48.8% | 52.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2703
- ORF length:
- 2634
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgcggccc cggagagccc gggagccgct gctcgtggcg ctgctgccgc 121 tggcgtggct ggcgcaggcg ggcctggcgc gcgcggcggg ctctgtgcgc ctggcgggcg 181 gcctgacgct gggcggcctg ttcccggtgc acgcgcgggg cgcggcgggc cgggcgtgcg 241 ggcagctgaa gaaggagcag ggcgtgcacc ggctggaggc catgctgtac gcgctggacc 301 gcgtcaacgc cgaccccgag ctgctgcccg gcgtgcgcct gggcgcgcgg ctgctggaca 361 cctgctcgcg ggacacctac gcgctggagc aggcgctgag cttcgtgcag gcgctgatcc 421 gcggccgcgg cgacggcgac gaggtgggcg tgcgctgccc gggaggcgtc cctccgctgc 481 gccccgcgcc ccccgagcgc gtcgtggccg tcgtgggcgc ctcggccagc tccgtctcca 541 tcatggtcgc caacgtgctg cgcctgtttg cgatacccca gatcagctat gcctccacag 601 ccccggagct cagcgactcc acacgctatg acttcttctc ccgggtggtg ccacccgact 661 cctaccaggc gcaggccatg gtggacatcg tgagggcact gggatggaac tatgtgtcca 721 cgctggcctc cgagggcaac tatggcgaaa gtggggttga ggccttcgtt cagatctccc 781 gagaggctgg gggggtctgt attgcccagt ctatcaagat tcccagggaa ccaaagccag 841 gagagttcag caaggtgatc aggagactca tggagacgcc caacgcccgg ggcatcatca 901 tctttgccaa tgaggatgac atcaggcggg tcctggaggc agctcgccag gccaacctga 961 ccggccactt cctgtgggtc ggctcagaca gctggggagc caagacctca cccatcttga 1021 gcctggagga cgtggccgtt ggggccatca ccatcctgcc caaaagggcc tccatcgacg 1081 gatttgacca gtacttcatg actcgatccc tggagaacaa ccgcaggaac atctggttcg 1141 ccgagttctg ggaagagaat tttaactgca aactgaccag ctcaggtacc cagtcagacg 1201 attccacccg caaatgcaca ggcgaggaac gcatcggccg ggactccacc tacgagcagg 1261 agggcaaggt gcagtttgtg attgatgcgg tgtacgccat tgcccacgcc ctccacagca 1321 tgcaccaggc gctctgccct gggcacacag gcctgtgccc ggcgatggaa cccactgatg 1381 ggcggatgct tctgcagtac attcgagctg tccgcttcaa tggcagcgca ggaacccctg 1441 tgatgttcaa cgagaacgga gatgcgcccg ggcggtacga catcttccag taccaggcga 1501 ccaatggcag tgccagcagt ggcgggtacc aggcagtggg ccagtgggca gagaccctca 1561 gactggatgt ggaggccctg cagtggtctg gcgaccccca cgaggtgccc tcgtctctgt 1621 gcagcctgcc ctgcgggccg ggggagcgga agaagatggt gaagggcgtc ccctgctgtt 1681 ggcactgcga ggcctgtgac gggtaccgct tccaggtgga cgagttcaca tgcgaggcct 1741 gtcctgggga catgaggccc acgcccaacc acacgggctg ccgccccaca cctgtggtgc 1801 gcctgagctg gtcctccccc tgggcagccc cgccgctcct cctggccgtg ctgggcatcg 1861 tggccactac cacggtggtg gccaccttcg tgcggtacaa caacacgccc atcgtccggg 1921 cctcgggccg agagctcagc tacgtcctcc tcaccggcat cttcctcatc tacgccatca 1981 ccttcctcat ggtggctgag cctggggccg cggtctgtgc cgcccgcagg ctcttcctgg 2041 gcctgggcac gaccctcagc tactctgccc tgctcaccaa gaccaaccgt atctaccgca 2101 tctttgagca gggcaagcgc tcggtcacac cccctccctt catcagcccc acctcacagc 2161 tggtcatcac cttcagcctc acctccctgc aggtggtggg gatgatagca tggctggggg 2221 cccggccccc acacagcgtg attgactatg aggaacagcg gacggtggac cccgagcagg 2281 ccagaggggt gctcaagtgc gacatgtcgg atctgtctct catcggctgc ctgggctaca 2341 gcctcctgct catggtcacg tgcacagtgt acgccatcaa ggcccgtggc gtgcccgaga 2401 ccttcaacga ggccaagccc atcggcttca ccatgtacac cacctgcatc atctggctgg 2461 cattcgtgcc catcttcttt GGCACTGCCC AGTCAGCTGA AAAGATCTAC ATCCAGACAA 2521 CCACGCTAAC CGTGTCCTTG AGCCTGAGTG CCTCGGTGTC CCTCGGCATG CTCTACGTAC 2581 CCAAAACCTA CGTCATCCTC TTCCATCCAG AGCAGAATGT GCAGAAGCGA AAGCGGAGCC 2641 TCAAGGCCAC CTCCACGGTG GCAGCCCCAC CCAAGGGCGA GGATGCAGAG GCCCACAAGG 2701 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2761 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2821 TTTATATATC TTGTGGAAAG GACGACTCGT ACGGCGAGAG TTATAGGCAA CGCGTTAAGT 2881 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt