Construct: ORF TRCN0000488053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020646.1_s317c1
- DNA Barcode:
- AAAACCCGGGAGCTCTATGTCGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ERBB2 (2064)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488053
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_004448.3 | 100% | 100% | |
| 2 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_001289936.1 | 98.6% | 98.3% | (many diffs) |
| 3 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_001005862.2 | 97.6% | 97.6% | 0_1ins90 |
| 4 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | XM_024450641.1 | 96.4% | 96.4% | 759_896del |
| 5 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | XM_024450642.1 | 95.2% | 94.8% | (many diffs) |
| 6 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | XM_024450643.1 | 94.1% | 94.1% | 0_1ins90;669_806del |
| 7 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_001289937.1 | 83.9% | 83.9% | 3161_3163delATAinsG;3165_3166ins602 |
| 8 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NR_110535.1 | 79.6% | 1_261del;484_546del;4090_4727del | |
| 9 | human | 2064 | ERBB2 | erb-b2 receptor tyrosine ki... | NM_001289938.1 | 48% | 48% | 0_1ins90;1809_1809delGins1867 |
| 10 | mouse | 13866 | Erbb2 | erb-b2 receptor tyrosine ki... | NM_001003817.1 | 85.6% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 3864
- ORF length:
- 3765
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccacta gtagcaccat ggagctggcg gccttgtgcc 121 gctgggggct cctcctcgcc ctcttgcccc ccggagccgc gagcacccaa gtgtgcaccg 181 gcacagacat gaagctgcgg ctccctgcca gtcccgagac ccacctggac atgctccgcc 241 acctctacca gggctgccag gtggtgcagg gaaacctgga actcacctac ctgcccacca 301 atgccagcct gtccttcctg caggatatcc aggaggtgca gggctacgtg ctcatcgctc 361 acaaccaagt gaggcaggtc ccactgcaga ggctgcggat tgtgcgaggc acccagctct 421 ttgaggacaa ctatgccctg gccgtgctag acaatggaga cccgctgaac aataccaccc 481 ctgtcacagg ggcctcccca ggaggcctgc gggagctgca gcttcgaagc ctcacagaga 541 tcttgaaagg aggggtcttg atccagcgga acccccagct ctgctaccag gacacgattt 601 tgtggaagga catcttccac aagaacaacc agctggctct cacactgata gacaccaacc 661 gctctcgggc ctgccacccc tgttctccga tgtgtaaggg ctcccgctgc tggggagaga 721 gttctgagga ttgtcagagc ctgacgcgca ctgtctgtgc cggtggctgt gcccgctgca 781 aggggccact gcccactgac tgctgccatg agcagtgtgc tgccggctgc acgggcccca 841 agcactctga ctgcctggcc tgcctccact tcaaccacag tggcatctgt gagctgcact 901 gcccagccct ggtcacctac aacacagaca cgtttgagtc catgcccaat cccgagggcc 961 ggtatacatt cggcgccagc tgtgtgactg cctgtcccta caactacctt tctacggacg 1021 tgggatcctg caccctcgtc tgccccctgc acaaccaaga ggtgacagca gaggatggaa 1081 cacagcggtg tgagaagtgc agcaagccct gtgcccgagt gtgctatggt ctgggcatgg 1141 agcacttgcg agaggtgagg gcagttacca gtgccaatat ccaggagttt gctggctgca 1201 agaagatctt tgggagcctg gcatttctgc cggagagctt tgatggggac ccagcctcca 1261 acactgcccc gctccagcca gagcagctcc aagtgtttga gactctggaa gagatcacag 1321 gttacctata catctcagca tggccggaca gcctgcctga cctcagcgtc ttccagaacc 1381 tgcaagtaat ccggggacga attctgcaca atggcgccta ctcgctgacc ctgcaagggc 1441 tgggcatcag ctggctgggg ctgcgctcac tgagggaact gggcagtgga ctggccctca 1501 tccaccataa cacccacctc tgcttcgtgc acacggtgcc ctgggaccag ctctttcgga 1561 acccgcacca agctctgctc cacactgcca accggccaga ggacgagtgt gtgggcgagg 1621 gcctggcctg ccaccagctg tgcgcccgag ggcactgctg gggtccaggg cccacccagt 1681 gtgtcaactg cagccagttc cttcggggcc aggagtgcgt ggaggaatgc cgagtactgc 1741 aggggctccc cagggagtat gtgaatgcca ggcactgttt gccgtgccac cctgagtgtc 1801 agccccagaa tggctcagtg acctgttttg gaccggaggc tgaccagtgt gtggcctgtg 1861 cccactataa ggaccctccc ttctgcgtgg cccgctgccc cagcggtgtg aaacctgacc 1921 tctcctacat gcccatctgg aagtttccag atgaggaggg cgcatgccag ccttgcccca 1981 tcaactgcac ccactcctgt gtggacctgg atgacaaggg ctgccccgcc gagcagagag 2041 ccagccctct gacgtccatc atctctgcgg tggttggcat tctgctggtc gtggtcttgg 2101 gggtggtctt tgggatcctc atcaagcgac ggcagcagaa gatccggaag tacacgatgc 2161 ggagactgct gcaggaaacg gagctggtgg agccgctgac acctagcgga gcgatgccca 2221 accaggcgca gatgcggatc ctgaaagaga cggagctgag gaaggtgaag gtgcttggat 2281 ctggcgcttt tggcacagtc tacaagggca tctggatccc tgatggggag aatgtgaaaa 2341 ttccagtggc catcaaagtg ttgagggaaa acacatcccc caaagccaac aaagaaatct 2401 tagacgaagc atacgtgatg gctggtgtgg gctccccata tgtctcccgc cttctgggca 2461 tctgcctgac atccacggtg cagctggtga cacagcttat gccctatggc tgcctcttag 2521 accatgtccg ggaaaaccgc ggacgcctgg gctcccagga cctgctgaac tggtgtatgc 2581 agattgccaa ggggatgagc tacctggagg atgtgcggct cgtacacagg gacttggccg 2641 ctcggaacgt gctggtcaag agtcccaacc atgtcaaaat tacagacttc gggctggctc 2701 ggctgctgga cattgacgag acagagtacc atgcagatgg gggcaaggtg cccatcaagt 2761 ggatggcgct ggagtccatt ctccgccggc ggttcaccca ccagagtgat gtgtggagtt 2821 atggtgtgac tgtgtgggag ctgatgactt ttggggccaa accttacgat gggatcccag 2881 cccgggagat ccctgacctg ctggaaaagg gggagcggct gccccagccc cccatctgca 2941 ccattgatgt ctacatgatc atggtcaaat gttggatgat tgactctgaa tgtcggccaa 3001 gattccggga gttggtgtct gaattctccc gcatggccag ggacccccag cgctttgtgg 3061 tcatccagaa tgaggacttg ggcccagcca gtcccttgga cagcaccttc taccgctcac 3121 tgctggagga cgatgacatg ggggacctgg tggatgctga ggagtatctg gtaccccagc 3181 agggcttctt ctgtccagac cctgccccgg gcgctggggg catggtccac cacaggcacc 3241 gcagctcatc taccaggagt ggcggtgggg acctgacact agggctggag ccctctgaag 3301 aggaggcccc caggtctcca ctggcaccct ccgaaggggc tggctccgat gtatttgatg 3361 gtgacctggg aatgggggca gccaaggggc tgcaaagcct ccccacacat gaccccagcc 3421 ctctacagcg gtacagtgag gaccccacag tacccctgcc ctctgagact gatggctacg 3481 ttgcccccct gacctgcagc ccccagcctg aatatgtgaa ccagccagat gttcggcccc 3541 agcccccttc gccccgagag ggccctctgc ctgctgcccg acctgctggt gccactctgg 3601 aaaggcccaa gactctcTCC CCAGGGAAGA ATGGGGTCGT CAAAGACGTT TTTGCCTTTG 3661 GGGGTGCCGT GGAGAACCCC GAGTACTTGA CACCCCAGGG AGGAGCTGCC CCTCAGCCCC 3721 ACCCTCCTCC TGCCTTCAGC CCAGCCTTCG ACAACCTCTA TTACTGGGAC CAGGACCCAC 3781 CAGAGCGGGG GGCTCCACCC AGCACCTTCA AAGGGACACC TACGGCAGAG AACCCAGAGT 3841 ACCTGGGTCT GGACGTGCCA GTGTGAAAGC TTAAGGGTGG GCGCGCCGAC CCAGCTTTCT 3901 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3961 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 4021 GTGGAAAGGA CGAAAAACCC GGGAGCTCTA TGTCGACACG CGTTAAGTCg acaatcaacc 4081 tctggattac aaaatttgtg aaagatt