Construct: ORF TRCN0000488060
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019888.1_s317c1
- DNA Barcode:
- GATATTCAGGGTCAGGTTACTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSF1R (1436)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488060
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_001288705.2 | 99.9% | 99.8% | 726C>T;2916_2917insG |
| 2 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_001349736.1 | 99.9% | 99.8% | 726C>T;2916_2917insG |
| 3 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_005211.3 | 99.9% | 99.8% | 726C>T;2916_2917insG |
| 4 | human | 1436 | CSF1R | colony stimulating factor 1... | NR_109969.1 | 70.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2991
- ORF length:
- 2919
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggccca ggagttctgc tgctcctgct ggtggccaca gcttggcatg 121 gtcagggaat cccagtgata gagcccagtg tccctgagct ggtcgtgaag ccaggagcaa 181 cggtgacctt gcgatgtgtg ggcaatggca gcgtggaatg ggatggcccc ccatcacctc 241 actggaccct gtactctgat ggctccagca gcatcctcag caccaacaac gctaccttcc 301 aaaacacggg gacctatcgc tgcactgagc ctggagaccc cctgggaggc agcgccgcca 361 tccacctcta tgtcaaagac cctgcccggc cctggaacgt gctagcacag gaggtggtcg 421 tgttcgagga ccaggacgca ctactgccct gtctgctcac agacccggtg ctggaagcag 481 gcgtctcgct ggtgcgtgtg cgtggccggc ccctcatgcg ccacaccaac tactccttct 541 cgccctggca tggcttcacc atccacaggg ccaagttcat tcagagccag gactatcaat 601 gcagtgccct gatgggtggc aggaaggtga tgtccatcag catccggctg aaagtgcaga 661 aagtcatccc agggccccca gccttgacac tggtgcctgc agagctggtg cggattcgag 721 gggaggctgc ccagatcgtg tgctcagcca gcagcgttga tgttaacttt gatgtcttcc 781 tccaacacaa caacactaag ctcgcaatcc ctcaacaatc tgactttcat aataaccgtt 841 accaaaaagt cctgaccctc aacctcgatc aagtagattt ccaacatgcc ggcaactact 901 cctgcgtggc cagcaacgtg cagggcaagc actccacctc catgttcttc cgggtggtag 961 agagtgccta cttgaacttg agctctgagc agaacctcat ccaggaggtg accgtggggg 1021 aggggctcaa cctcaaagtc atggtggagg cctacccagg cctgcaaggt tttaactgga 1081 cctacctggg acccttttct gaccaccagc ctgagcccaa gcttgctaat gctaccacca 1141 aggacacata caggcacacc ttcaccctct ctctgccccg cctgaagccc tctgaggctg 1201 gccgctactc cttcctggcc agaaacccag gaggctggag agctctgacg tttgagctca 1261 cccttcgata ccccccagag gtaagcgtca tatggacatt catcaacggc tctggcaccc 1321 ttttgtgtgc tgcctctggg tacccccagc ccaacgtgac atggctgcag tgcagtggcc 1381 acactgatag gtgtgatgag gcccaagtgc tgcaggtctg ggatgaccca taccctgagg 1441 tcctgagcca ggagcccttc cacaaggtga cggtgcagag cctgctgact gttgagacct 1501 tagagcacaa ccaaacctac gagtgcaggg cccacaacag cgtggggagt ggctcctggg 1561 ccttcatacc catctctgca ggagcccaca cgcatccccc ggatgagttc ctcttcacac 1621 cagtggtggt cgcctgcatg tccatcatgg ccttgctgct gctgctgctc ctgctgctat 1681 tgtacaagta taagcagaag cccaagtacc aggtccgctg gaagatcatc gagagctatg 1741 agggcaacag ttatactttc atcgacccca cgcagctgcc ttacaacgag aagtgggagt 1801 tcccccggaa caacctgcag tttggtaaga ccctcggagc tggagccttt gggaaggtgg 1861 tggaggccac ggcctttggt ctgggcaagg aggatgctgt cctgaaggtg gctgtgaaga 1921 tgctgaagtc cacggcccat gctgatgaga aggaggccct catgtccgag ctgaagatca 1981 tgagccacct gggccagcac gagaacatcg tcaaccttct gggagcctgt acccatggag 2041 gccctgtact ggtcatcacg gagtactgtt gctatggcga cctgctcaac tttctgcgaa 2101 ggaaggctga ggccatgctg ggacccagcc tgagccccgg ccaggacccc gagggaggcg 2161 tcgactataa gaacatccac ctcgagaaga aatatgtccg cagggacagt ggcttctcca 2221 gccagggtgt ggacacctat gtggagatga ggcctgtctc cacttcttca aatgactcct 2281 tctctgagca agacctggac aaggaggatg gacggcccct ggagctccgg gacctgcttc 2341 acttctccag ccaagtagcc cagggcatgg ccttcctcgc ttccaagaat tgcatccacc 2401 gggacgtggc agcgcgtaac gtgctgttga ccaatggtca tgtggccaag attggggact 2461 tcgggctggc tagggacatc atgaatgact ccaactacat tgtcaagggc aatgcccgcc 2521 tgcctgtgaa gtggatggcc ccagagagca tctttgactg tgtctacacg gttcagagcg 2581 acgtctggtc ctatggcatc ctcctctggg agatcttctc acttgggctg aatccctacc 2641 ctggcatcct ggtgaacagc aagttctata aactggtgaa ggatggatac caaatggccc 2701 agcctgcatt tgccccaaag aatatataca gcatcatgca ggccTGCTGG GCCTTGGAGC 2761 CCACCCACAG ACCCACCTTC CAGCAGATCT GCTCCTTCCT TCAGGAGCAG GCCCAAGAGG 2821 ACAGGAGAGA GCGGGACTAT ACCAATCTGC CGAGCAGCAG CAGAAGCGGT GGCAGCGGCA 2881 GCAGCAGCAG TGAGCTGGAG GAGGAGAGCT CTAGTGAGCA CCTGACCTGC TGCGAGCAAG 2941 GGGATATCGC CCAGCCCTTG CTGCAGCCCA ACAACTATCA GTTCTGCGAC CCAGCTTTCT 3001 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3061 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3121 GTGGAAAGGA CGAGATATTC AGGGTCAGGT TACTTTCACG CGTTAAGTCg acaatcaacc 3181 tctggattac aaaatttgtg aaagatt