Construct: ORF TRCN0000488074
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020906.1_s317c1
- DNA Barcode:
- TGTCAGAGCTTCTATTACTCACCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR20 (2843)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488074
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2843 | GPR20 | G protein-coupled receptor 20 | NM_005293.3 | 99.7% | 99.7% | 465C>T;624C>T;689A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1146
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccctct gtgtctccag cggggccctc ggccggggca gtccccaatg 121 ccaccgcagt gacaacagtg cggaccaatg ccagcgggct ggaggtgccc ctgttccacc 181 tgtttgcccg gctggacgag gagctgcatg gcaccttccc aggcctgtgg ctggcgctga 241 tggcggtgca cggagccatc ttcctggcag ggctggtgct caacgggctg gcgctgtacg 301 tcttctgctg ccgcacccgg gccaagacac cctcagtcat ctacaccatc aacctggtgg 361 tgaccgatct actggtaggg ctgtccctgc ccacgcgctt cgctgtgtac tacggcgcca 421 ggggctgcct gcgctgtgcc ttcccgcacg tcctcggtta cttcctcaac atgcactgct 481 ccatcctctt cctcacctgc atctgcgtgg accgctacct ggccatcgtg cggcctgaag 541 gctcccgccg ctgccgccag cctgcctgtg ccagggccgt gtgcgccttc gtgtggctgg 601 ccgccggtgc cgtcaccctg tcggtgctgg gcgtgacagg cagccggccc tgctgccgtg 661 tctttgcgct gactgtcctg gagttcctgc tgcctctgct ggtcatcagc gtgtttaccg 721 gccgcatcat gtgtgcactg tcgcggccgg gtctgctccg ccagggtcgc cagcgccgcg 781 tgcgggccat gcagctcctg ctcacggtgc tcatcatctt tctcgtctgc ttcacgcccT 841 TCCACGCCCG CCAAGTGGCC GTGGCGCTGT GGCCCGACAT GCCACACCAC ACGAGCCTCG 901 TGGTCTACCA CGTGGCCGTG ACCCTCAGCA GCCTCAACAG CTGCATGGAC CCCATCGTCT 961 ACTGCTTCGT CACCAGTGGC TTCCAGGCCA CCGTCCGAGG CCTCTTCGGC CAGCACGGAG 1021 AGCGTGAGCC CAGCAGCGGT GACGTGGTCA GCATGCACAG GAGCTCCAAG GGCTCAGGCC 1081 GTCATCACAT CCTCAGTGCC GGCCCTCACG CCCTCACCCA GGCCCTGGCT AATGGGCCCG 1141 AGGCTTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGTCAG AGCTTCTATT ACTCACCAAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt