Construct: ORF TRCN0000488077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020493.1_s317c1
- DNA Barcode:
- GTCTTAATTTTCTTGTACCTGACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PLK3 (1263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488077
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1263 | PLK3 | polo like kinase 3 | NM_004073.4 | 100% | 100% | |
| 2 | human | 1263 | PLK3 | polo like kinase 3 | XR_246234.4 | 65.2% | 1_512del;2017_2018ins130;2321_2640del | |
| 3 | mouse | 12795 | Plk3 | polo-like kinase 3 | NM_013807.3 | 87.9% | 93.2% | (many diffs) |
| 4 | mouse | 12795 | Plk3 | polo-like kinase 3 | NM_001313916.1 | 85.7% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2010
- ORF length:
- 1938
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcct gccgccggtt tcctgtctcc gcgccccttc cagcgtgcgg 121 ccgccgcgcc cgctcccccg gccgggcccg ggccgcctcc gagtgccttg cgcggacctg 181 agctggagat gctggccggg ctaccgacgt cagaccccgg gcgcctcatc acggacccgc 241 gcagcggccg cacctacctc aaaggccgct tgttgggcaa ggggggcttc gcccgctgct 301 acgaggccac tgacacagag actggcagcg cctacgctgt caaagtcatc ccgcagagcc 361 gcgtcgccaa gccgcatcag cgcgagaaga tcctaaatga gattgagctg caccgagacc 421 tgcagcaccg ccacatcgtg cgtttttcgc accactttga ggacgctgac aacatctaca 481 ttttcttgga gctctgcagc cgaaagtccc tggcccacat ctggaaggcc cggcacaccc 541 tgttggagcc agaagtgcgc tactacctgc ggcagatcct ttctggcctc aagtacttgc 601 accagcgcgg catcttgcac cgggacctca agttgggaaa ttttttcatc actgagaaca 661 tggaactgaa ggtgggggat tttgggctgg cagcccggtt ggagcctccg gagcagagga 721 agaagaccat ctgtggcacc cccaactatg tggctccaga agtgctgctg agacagggcc 781 acggccctga ggcggatgta tggtcactgg gctgtgtcat gtacacgctg ctctgcggga 841 gccctccctt tgagacggct gacctgaagg agacgtaccg ctgcatcaag caggttcact 901 acacgctgcc tgccagcctc tcactgcctg cccggcagct cctggccgcc atccttcggg 961 cctcaccccg agaccgcccc tctattgacc agatcctgcg ccatgacttc tttaccaagg 1021 gctacacccc cgatcgactc cctatcagca gctgcgtgac agtcccagac ctgacacccc 1081 ccaacccagc taggagtctg tttgccaaag ttaccaagag cctctttggc agaaagaaga 1141 agagtaagaa tcatgcccag gagagggatg aggtctccgg tttggtgagc ggcctcatgc 1201 gcacatccgt tggccatcag gatgccaggc cagaggctcc agcagcttct ggcccagccc 1261 ctgtcagcct ggtagagaca gcacctgaag acagctcacc ccgtgggaca ctggcaagca 1321 gtggagatgg atttgaagaa ggtctgactg tggccacagt agtggagtca gccctttgtg 1381 ctctgagaaa ttgtatagcc ttcatgcccc cagcggaaca gaacccggcc cccctggccc 1441 agccagagcc tctggtgtgg gtcagcaagt gggttgacta ctccaataag ttcggctttg 1501 ggtatcaact gtccagccgc cgtgtggctg tgctcttcaa cgatggcaca catatggccc 1561 tgtcggccaa cagaaagact gtgcactaca atcccaccag cacaaagcac ttctccttct 1621 ccgtgggtgc tgtgccccgg gccctgcagc ctcagctggg tatcctgcgg tacttcgcct 1681 cctacatgga gcagcacctc atgaagggtg gagatctgcc cagtgtggaa gaggtagagg 1741 tacctgcTCC GCCCTTGCTG CTGCAGTGGG TCAAGACGGA TCAGGCTCTC CTCATGCTGT 1801 TTAGTGATGG CACTGTCCAG GTGAACTTCT ACGGGGACCA CACCAAGCTG ATTCTCAGTG 1861 GCTGGGAGCC CCTCCTTGTG ACTTTTGTGG CCCGAAATCG TAGTGCTTGT ACTTACCTCG 1921 CTTCCCACCT TCGGCAGCTG GGCTGCTCTC CAGACCTGCG GCAGCGACTC CGCTATGCTC 1981 TGCGCCTGCT CCGGGACCGC AGCCCAGCCT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG 2041 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2101 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 2161 CTTAATTTTC TTGTACCTGA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2221 ttgtgaaaga tt