Construct: ORF TRCN0000488099

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019734.1_s317c1
DNA Barcode:
AGATGGCCCCTTTGTAGTATGAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PAK6 (56924)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488099
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 106821730 BUB1B-PAK6 BUB1B-PAK6 readthrough NM_001128628.2 99.9% 99.8% 249G>A;2043_2044insG
2 human 106821730 BUB1B-PAK6 BUB1B-PAK6 readthrough NM_001128629.3 99.9% 99.8% 249G>A;2043_2044insG
3 human 56924 PAK6 p21 (RAC1) activated kinase 6 NM_001276717.1 99.9% 99.8% 249G>A;2043_2044insG
4 human 56924 PAK6 p21 (RAC1) activated kinase 6 NM_020168.5 99.9% 99.8% 249G>A;2043_2044insG
5 human 56924 PAK6 p21 (RAC1) activated kinase 6 NM_001276718.2 93.2% 93.2% 249G>A;1741_1742ins135;1908_1909insG
6 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... NM_001033254.3 88.6% 92.3% (many diffs)
7 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... NM_001145854.1 88.6% 92.3% (many diffs)
8 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... XM_011239419.1 88.6% 92.3% (many diffs)
9 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... XM_011239420.2 88.6% 92.3% (many diffs)
10 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... XR_866265.2 51.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2118
ORF length:
2046
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgttccgc aagaaaaaga agaaacgccc tgagatctca gcgccacaga
121acttccagca ccgtgtccac acctccttcg accccaaaga aggcaagttt gtgggcctcc
181ccccacaatg gcagaacatc ctggacacac tgcggcgccc caagcccgtg gtggaccctt
241cgcgaatcac acgggtgcag ctccagccca tgaagacagt ggtgcggggc agcgcgatgc
301ctgtggatgg ctacatctca gggctgctca acgacatcca gaagttgtca gtcatcagct
361ccaacaccct gcgtggccgc agccccacca gccggcggcg ggcacagtcc ctggggctgc
421tgggggatga gcactgggcc accgacccag acatgtacct ccagagcccc cagtctgagc
481gcactgaccc ccacggcctc tacctcagct gcaacggggg cacaccagca ggccacaagc
541agatgccgtg gcccgagcca cagagcccac gggtcctgcc caatgggctg gctgcaaagg
601cacagtccct gggccccgcc gagtttcagg gtgcctcgca gcgctgtctg cagctgggtg
661cctgcctgca gagctcccca ccaggagcct cgccccccac gggcaccaat aggcatggaa
721tgaaggctgc caagcatggc tctgaggagg cccggccaca gtcctgcctg gtgggctcag
781ccacaggcag gccaggtggg gaaggcagcc ctagccctaa gacccgggag agcagcctga
841agcgcaggct attccgaagc atgttcctgt ccactgctgc cacagcccct ccaagcagca
901gcaagccagg ccctccacca cagagcaagc ccaactcctc tttccgaccg ccgcagaaag
961acaacccccc aagcctggtg gccaaggccc agtccttgcc ctcggaccag ccggtgggga
1021ccttcagccc tctgaccact tcggatacca gcagccccca gaagtccctc cgcacagccc
1081cggccacagg ccagcttcca ggccggtctt ccccagcggg atccccccgc acctggcacg
1141cccagatcag caccagcaac ctgtacctgc cccaggaccc cacggttgcc aagggtgccc
1201tggctggtga ggacacaggt gttgtgacac atgagcagtt caaggctgcg ctcaggatgg
1261tggtggacca gggtgacccc cggctgctgc tggacagcta cgtgaagatt ggcgagggct
1321ccaccggcat cgtctgcttg gcccgggaga agcactcggg ccgccaggtg gccgtcaaga
1381tgatggacct caggaagcag cagcgcaggg agctgctctt caacgaggtg gtgatcatgc
1441gggactacca gcacttcaac gtggtggaga tgtacaagag ctacctggtg ggcgaggagc
1501tgtgggtgct catggagttc ctgcagggag gagccctcac agacatcgtc tcccaagtca
1561ggctgaatga ggagcagatt gccactgtgt gtgaggctgt gctgcaggcc ctggcctacc
1621tgcatgctca gggtgtcatc caccgggaca tcaagagtga ctccatcctg ctgaccctcg
1681atggcagggt gaagctctcg gacttcggat tctgtgctca gatcagcaaa gacgtcccta
1741agaggaagtc cctggtggga accccctact ggatggctcc TGAAGTGATC TCCAGGTCTT
1801TGTATGCCAC TGAGGTGGAT ATCTGGTCTC TGGGCATCAT GGTGATTGAG ATGGTAGATG
1861GGGAGCCACC GTACTTCAGT GACTCCCCAG TGCAAGCCAT GAAGAGGCTC CGGGACAGCC
1921CCCCACCCAA GCTGAAAAAC TCTCACAAGG TCTCCCCAGT GCTGCGAGAC TTCCTGGAGC
1981GGATGCTGGT GCGGGACCCC CAAGAGAGAG CCACAGCCCA GGAGCTCCTA GACCACCCCT
2041TCCTGCTGCA GACAGGGCTA CCTGAGTGCC TGGTGCCCCT GATCCAGCTC TACCGAAAGC
2101AGACCTCCAC CTGCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2161CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2221TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGATGGCCCC TTTGTAGTAT
2281GAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)