Construct: ORF TRCN0000488112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019217.1_s317c1
- DNA Barcode:
- TGTGACCAACCCGGTCATCACTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R60 (338398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338398 | TAS2R60 | taste 2 receptor member 60 | NM_177437.1 | 99.8% | 100% | 930T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1029
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaat ggagaccaca tggttctagg atcttcggtg actgacaaga 121 aggccatcat cttggttacc attttactcc ttttacgcct ggtagcaata gcaggcaatg 181 gcttcatcac tgctgctctg ggcgtggagt gggtgctacg gagaatgttg ttgccttgtg 241 ataagttatt ggttagccta ggggcctctc gcttctgtct gcagtcagtg gtaatgggta 301 agaccattta tgttttcttg catccgatgg ccttcccata caaccctgta ctgcagtttc 361 tagctttcca gtgggacttc ctgaatgctg ccaccttatg gtcctctacc tggctcagtg 421 tcttctattg tgtgaaaatt gctaccttca cccaccctgt cttcttctgg ctaaagcaca 481 agttgtctgg gtggctacca tggatgctct tcagctctgt agggctctcc agcttcacca 541 ccattctatt tttcataggc aaccacagaa tgtatcagaa ctatttaagg aaccatctac 601 aaccttggaa tgtcactggc gatagcatac ggagctactg tgagaaattc tatctcttcc 661 ctctaaaaat gattacttgg acaatgccca ctgctgtctt tttcatttgc atgattttgc 721 tcatcacatc tctgggaaga cacaggaaga aggctctcct tacaacctCA GGATTCCGAG 781 AGCCCAGTGT GCAGGCACAC ATAAAGGCTC TGCTGGCTCT CCTCTCTTTT GCCATGCTCT 841 TCATCTCATA TTTCCTGTCA CTGGTGTTCA GTGCTGCAGG TATTTTTCCA CCTCTGGACT 901 TTAAATTCTG GGTGTGGGAG TCAGTGATTT ATCTGTGTGC AGCAGTTCAC CCCATCATTC 961 TGCTCTTCAG CAACTGCAGG CTGAGAGCTG TGCTGAAGAG TCGCCGTTCC TCAAGGTGTG 1021 GGACACCTGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGTGAC CAACCCGGTC ATCACTCGAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt