Construct: ORF TRCN0000488114
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019074.1_s317c1
- DNA Barcode:
- GCATGCCATCTCCAACTCCATACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DCLK3 (85443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488114
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85443 | DCLK3 | doublecortin like kinase 3 | NM_033403.1 | 99.1% | 2% | (many diffs) |
| 2 | human | 85443 | DCLK3 | doublecortin like kinase 3 | XM_011534167.2 | 99.1% | 2% | (many diffs) |
| 3 | human | 85443 | DCLK3 | doublecortin like kinase 3 | XM_011534168.3 | 90.5% | 2.1% | (many diffs) |
| 4 | human | 85443 | DCLK3 | doublecortin like kinase 3 | XM_011534169.1 | 75.2% | 2.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 192
- ORF length:
- 120
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggtgat accgaagagc attcaggtgg ctgtagaaga actgtacccc 121 aacaaagccc gggccctgac actggcccag cacagccgtg ccccttctcc aaggctgagg 181 agcaggctgt ttagcaaggc tctgaaagga gaccaccgct gtggggagac cgagaccccc 241 atgagctgca gcgaagttgc aggatgcaag gcagccatga ggcaccaggg gaagatcccc 301 gaggagcttt cactagatga cagagcgagg acccagaaga agtgggggag ggggaaatgg 361 gagccagaac ccagtagcaa gccccccagg gaagccactc tggaagagag gcacgcgagg 421 ggagagaagc atcttggggt ggagattgaa aagacctcgg gtgaaattat cagatgcgag 481 aagtgcaaga gagagaggga gctccagcag agcctggagc gtgagaggct ttctctgggg 541 accagtgagc tggatatggg gaagggccca atgtatgatg tggagaagct ggtgaggacc 601 agaagctgca ggaggtctcc cgaggcaaat cctgcaagtg gggaggaagg gtggaagggt 661 gacagccaca ggagcagccc caggaatccc actcaagagc tgaggagacc cagcaagagc 721 atggacaaga aagaggacag aggcccagag gatcaagaaa gccatgctca gggagcagcc 781 aaggccaaga aggaccttgt ggaagttctt cctgtcacag aggaggggct gagggaggtg 841 aagaaggaca ccaggcccat gagcaggagc aaacatggtg gctggctcct gagagagcac 901 caggcgggct ttgagaagct ccgcaggacc cgaggagaag agaaggaggc agagaaggag 961 aaaaagccat gtatgtctgg aggcagaagg atgactctca gagatgacca acctgcaaag 1021 ctagaaaagg agcccaagac gaggccagaa gagaacaagc cagagcggcc cagcggtcgg 1081 aagccacggc ccatgggcat cattgccgcc aatgtggaaa agcattatga gactggccgg 1141 gtcattgggg atgggaactt tgctgtcgtg aaggagtgca gacaccgcga gaccaggcag 1201 gcctatgcga tgaagatcat tgacaagtcc agactcaagg gcaaggagga catggtggac 1261 agtgagatct tgatcatcca gagcctctct caccccaaca tcgtgaaatt gcatgaagtc 1321 tacgaaacag acatggaaat ctacctgatc ctggagtacg tgcagggagg agaccttttt 1381 gacgccatca tagaaagtgt gaagttcccg gagcccgatg ctgccctcat gatcatggac 1441 ttatgcaaag ccctcgtcca catgcacgac aagagcattg tccaccggga cctcaagccg 1501 gaaaaccttt tggttcagcg aaatgaggac aaatctacta ccttgaaatt ggctgatttt 1561 ggacttgcaa agcatgtggt gagacctata tttactgtgt gtgggacccc aacttacgta 1621 gctcccgaaa ttctttctga gaaaggttat ggactggagg tggacatgtg ggctgctggc 1681 gtgatcctct atatcctgct gtgtggcttt cccccattcc gcagcccTGA GAGGGACCAG 1741 GACGAGCTCT TTAACATCAT CCAGCTGGGC CACTTTGAGT TCCTCCCCCC TTACTGGGAC 1801 AATATCTCTG ATGCTGCTAA AGATCTGGTG AGCCGGTTGC TGGTGGTAGA CCCCAAAAAG 1861 CGCTACACAG CTCATCAGGT TCTTCAGCAC CCCTGGATCG AAACAGCTGG CAAGACCAAT 1921 ACAGTGAAAC GACAGAAGCA GGTGTCCCCC AGCAGCGAGG GTCACTTCCG GAGCCAGCAC 1981 AAGAGGGTTG TGGAGCAGGT ATCAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT 2041 AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA 2101 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCATGCCATC 2161 TCCAACTCCA TACAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 2221 gatt