Construct: ORF TRCN0000488116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020029.1_s317c1
- DNA Barcode:
- GGAACCGAGAATTTTTTAAATAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JAK3 (3718)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3718 | JAK3 | Janus kinase 3 | NM_000215.3 | 99.9% | 99.9% | 3372_3373insG |
| 2 | human | 3718 | JAK3 | Janus kinase 3 | XM_005259896.3 | 96.2% | 96.2% | 1_129del;3501_3502insG |
| 3 | human | 3718 | JAK3 | Janus kinase 3 | XM_011527990.1 | 69.1% | 67.5% | (many diffs) |
| 4 | human | 3718 | JAK3 | Janus kinase 3 | XM_011527991.2 | 51.5% | 51.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3447
- ORF length:
- 3375
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcacct ccaagtgaag agacgcccct gatccctcag cgttcatgca 121 gcctcttgtc cacggaggct ggtgccctgc atgtgctgct gcccgctcgg ggccccgggc 181 ccccccagcg cctatctttc tcctttgggg accacttggc tgaggacctg tgcgtgcagg 241 ctgccaaggc cagcggcatc ctgcctgtgt accactccct ctttgctctg gccacggagg 301 acctgtcctg ctggttcccc ccgagccaca tcttctccgt ggaggatgcc agcacccaag 361 tcctgctgta caggattcgc ttttacttcc ccaattggtt tgggctggag aagtgccacc 421 gcttcgggct acgcaaggat ttggccagtg ctatccttga cctgccagtc ctggagcacc 481 tctttgccca gcaccgcagt gacctggtga gtgggcgcct ccccgtgggc ctcagtctca 541 aggagcaggg tgagtgtctc agcctggccg tgttggacct ggcccggatg gcgcgagagc 601 aggcccagcg gccgggagag ctgctgaaga ctgtcagcta caaggcctgc ctacccccaa 661 gcctgcgcga cctgatccag ggcctgagct tcgtgacgcg gaggcgtatt cggaggacgg 721 tgcgcagagc cctgcgccgc gtggccgcct gccaggcaga ccggcactcg ctcatggcca 781 agtacatcat ggacctggag cggctggatc cagccggggc cgccgagacc ttccacgtgg 841 gcctccctgg ggcccttggt ggccacgacg ggctggggct gctccgcgtg gctggtgacg 901 gcggcatcgc ctggacccag ggagaacagg aggtcctcca gcccttctgc gactttccag 961 aaatcgtaga cattagcatc aagcaggccc cgcgcgttgg cccggccgga gagcaccgcc 1021 tggtcactgt taccaggaca gacaaccaga ttttagaggc cgagttccca gggctgcccg 1081 aggctctgtc gttcgtggcg ctcgtggacg gctacttccg gctgaccacg gactcccagc 1141 acttcttctg caaggaggtg gcaccgccga ggctgctgga ggaagtggcc gagcagtgcc 1201 acggccccat cactctggac tttgccatca acaagctcaa gactgggggc tcacgtcctg 1261 gctcctatgt tctccgccgc agcccccagg actttgacag cttcctcctc actgtctgtg 1321 tccagaaccc ccttggtcct gattataagg gctgcctcat ccggcgcagc cccacaggaa 1381 ccttccttct ggttggcctc agccgacccc acagcagtct tcgagagctc ctggcaacct 1441 gctgggatgg ggggctgcac gtagatgggg tggcagtgac cctcacttcc tgctgtatcc 1501 ccagacccaa agaaaagtcc aacctgatcg tggtccagag aggtcacagc ccacccacat 1561 catccttggt tcagccccaa tcccaatacc agctgagtca gatgacattt cacaagatcc 1621 ctgctgacag cctggagtgg catgagaacc tgggccatgg gtccttcacc aagatttacc 1681 ggggctgtcg ccatgaggtg gtggatgggg aggcccgaaa gacagaggtg ctgctgaagg 1741 tcatggatgc caagcacaag aactgcatgg agtcattcct ggaagcagcg agcttgatga 1801 gccaagtgtc gtaccggcat ctcgtgctgc tccacggcgt gtgcatggct ggagacagca 1861 ccatggtgca ggaatttgta cacctggggg ccatagacat gtatctgcga aaacgtggcc 1921 acctggtgcc agccagctgg aagctgcagg tggtcaaaca gctggcctac gccctcaact 1981 atctggagga caaaggcctg ccccatggca atgtctctgc ccggaaggtg ctcctggctc 2041 gggagggggc tgatgggagc ccgcccttca tcaagctgag tgaccctggg gtcagccccg 2101 ctgtgttaag cctggagatg ctcaccgaca ggatcccctg ggtggccccc gagtgtctcc 2161 gggaggcgca gacacttagc ttggaagctg acaagtgggg cttcggcgcc acggtctggg 2221 aagtgtttag tggcgtcacc atgcccatca gtgccctgga tcctgctaag aaactccaat 2281 tttatgagga ccggcagcag ctgccggccc ccaagtggac agagctggcc ctgctgattc 2341 aacagtgcat ggcctatgag ccggtccaga ggccctcctt ccgagccgtc attcgtgacc 2401 tcaatagcct catctcttca gactatgagc tcctctcaga ccccacacct ggtgccctgg 2461 cacctcgtga tgggctgtgg aatggtgccc agctctatgc ctgccaagac cccacgatct 2521 tcgaggagag acacctcaag tacatctcac agctgggcaa gggcaacttt ggcagcgtgg 2581 agctgtgccg ctatgacccg ctaggcgaca atacaggtgc cctggtggcc gtgaaacagc 2641 tgcagcacag cgggccagac cagcagaggg actttcagcg ggagattcag atcctcaaag 2701 cactgcacag tgatttcatt gtcaagtatc gtggtgtcag ctatggcccg ggccgccaga 2761 gcctgcggct ggtcatggag tacctgccca gcggctgctt gcgcgacttc ctgcagcggc 2821 accgcgcgcg cctcgatgcc agccgcctcc ttctctattc ctcgcagatc tgcaagggca 2881 tggagtacct gggctcccgc cgctgcgtgc accgcgacct ggccgcccga aacatcctcg 2941 tggagagcga ggcacacgtc aagatcgctg acttcggcct agctaagctg ctgccgcttg 3001 acaaagacta ctacgtggtc cgcgagccag gccagagccc cattttctgg tatgcccccg 3061 aatccctctc ggacaacatc ttctctcgcc agtcagacgt ctggagcttc ggggtcgtcc 3121 tgtacgagct cttcacctac tgcgacaaaa gctgcagccc ctcggccgag ttcctgcgga 3181 tgatgggatg tgagcgggat gtccccgccc tCTGCCGCCT CTTGGAACTG CTGGAGGAGG 3241 GCCAGAGGCT GCCGGCGCCT CCTGCCTGCC CTGCTGAGGT TCACGAGCTC ATGAAGCTGT 3301 GCTGGGCCCC TAGCCCACAG GACCGGCCAT CATTCAGCGC CCTGGGCCCC CAGCTGGACA 3361 TGCTGTGGAG CGGAAGCCGG GGGTGTGAGA CTCATGCCTT CACTGCTCAC CCAGAGGGCA 3421 AACACCACTC CCTGTCCTTT TCAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GAACCGAGAA 3601 TTTTTTAAAT AAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3661 att