Construct: ORF TRCN0000488129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019419.1_s317c1
- DNA Barcode:
- AAGACTCCTCTCGAATGCCACCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGTR2 (186)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488129
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 186 | AGTR2 | angiotensin II receptor type 2 | NM_000686.5 | 99.8% | 99.7% | 51T>C;1089_1090insG |
| 2 | human | 186 | AGTR2 | angiotensin II receptor type 2 | XM_011537533.1 | 99.8% | 99.7% | 51T>C;1089_1090insG |
| 3 | mouse | 11609 | Agtr2 | angiotensin II receptor, ty... | NM_007429.5 | 88.8% | 91.4% | (many diffs) |
| 4 | mouse | 11609 | Agtr2 | angiotensin II receptor, ty... | XM_006527555.1 | 82% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1167
- ORF length:
- 1092
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaag ggcaactcca cccttgccac tactagcaaa aacattacca 121 gcggccttca cttcgggctt gtgaacatct ctggcaacaa tgagtctacc ttgaactgtt 181 cacagaaacc atcagataag catttagatg caattcctat tctttactac attatatttg 241 taattggatt tctggtcaat attgtcgtgg ttacactgtt ttgttgtcaa aagggtccta 301 aaaaggtttc tagcatatac atcttcaacc tcgctgtggc tgatttactc cttttggcta 361 ctcttcctct atgggcaacc tattattctt atagatatga ctggctcttt ggacctgtga 421 tgtgcaaagt ttttggttct tttcttaccc tgaacatgtt tgcaagcatt ttttttatca 481 cctgcatgag tgttgatagg taccaatctg tcatctaccc ctttctgtct caaagaagaa 541 atccctggca agcatcttat atagttcccc ttgtttggtg tatggcctgt ttgtcctcat 601 tgccaacatt ttattttcga gacgtcagaa ccattgaata cttaggagtg aatgcttgca 661 ttatggcttt cccacctgag aaatatgccc aatggtcagc tgggattgcc ttaatgaaaa 721 atatccttgg ttttattatc cctttaatat tcatagcaac atgctatttt ggaattagaa 781 aacacttact gaagacgaat agctatggga agaacaggat aacccgtgac caagtcctga 841 agatggcagc tgctgttgtt ctggccttca tcatttgctg gcttcccttc catgttctga 901 ccTTCCTGGA TGCTCTGGCC TGGATGGGTG TCATTAATAG CTGCGAAGTT ATAGCAGTCA 961 TTGACCTGGC ACTTCCTTTT GCCATCCTCT TGGGATTCAC CAACAGCTGC GTTAATCCGT 1021 TTCTGTATTG TTTTGTTGGA AACCGGTTCC AACAGAAGCT CCGCAGTGTG TTTAGGGTTC 1081 CAATTACTTG GCTCCAAGGG AAAAGAGAGA GTATGTCTTG CCGGAAAAGC AGTTCTCTTA 1141 GAGAAATGGA GACCTTTGTG TCTGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1201 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1261 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA AGACTCCTCT 1321 CGAATGCCAC CCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1381 att