Construct: ORF TRCN0000488175
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021135.1_s317c1
- DNA Barcode:
- GGTGTGACAAATGAAATGGTGGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GHSR (2693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488175
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2693 | GHSR | growth hormone secretagogue... | NM_198407.2 | 99.8% | 100% | 171C>T;477G>A |
| 2 | human | 2693 | GHSR | growth hormone secretagogue... | NM_004122.2 | 77% | 71.7% | (many diffs) |
| 3 | mouse | 208188 | Ghsr | growth hormone secretagogue... | NM_177330.4 | 89.5% | 95.3% | (many diffs) |
| 4 | mouse | 208188 | Ghsr | growth hormone secretagogue... | XM_017319513.1 | 76.3% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1170
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtggaac gcgacgccca gcgaagagcc ggggttcaac ctcacactgg 121 ccgacctgga ctgggatgct tcccccggca acgactcgct gggcgacgag ctgctgcagc 181 tcttccccgc gccgctgctg gcgggcgtca cagccacctg cgtggcactc ttcgtggtgg 241 gtatcgctgg caacctgctc accatgctgg tggtgtcgcg cttccgcgag ctgcgcacca 301 ccaccaacct ctacctgtcc agcatggcct tctccgatct gctcatcttc ctctgcatgc 361 ccctggacct cgttcgcctc tggcagtacc ggccctggaa cttcggcgac ctcctctgca 421 aactcttcca attcgtcagt gagagctgca cctacgccac ggtgctcacc atcacagcgc 481 tgagcgtcga gcgctacttc gccatctgct tcccactccg ggccaaggtg gtggtcacca 541 aggggcgagt gaagctggtc atcttcgtca tctgggccgt ggccttctgc agcgccgggc 601 ccatcttcgt gctagtcggg gtggagcacg agaacggcac cgacccttgg gacaccaacg 661 agtgccgccc caccgagttt gcggtgcgct ctggactgct cacggtcatg gtgtgggtgt 721 ccagcatctt cttcttcctt cctgtcttct gtctcacggt cctctacagt ctcatcggca 781 ggaagctgtg gcggaggagg cgcggcgatg ctgtcgtggg tgcctcgctc agggaccaga 841 acCACAAGCA AACCGTGAAA ATGCTGGCTG TAGTGGTGTT TGCCTTCATC CTCTGCTGGC 901 TCCCCTTCCA CGTAGGGCGA TATTTATTTT CCAAATCCTT TGAGCCTGGC TCCTTGGAGA 961 TTGCTCAGAT CAGCCAGTAC TGCAACCTCG TGTCCTTTGT CCTCTTCTAC CTCAGTGCTG 1021 CCATCAACCC CATTCTGTAC AACATCATGT CCAAGAAGTA CCGGGTGGCA GTGTTCAGAC 1081 TTCTGGGATT CGAACCCTTC TCCCAGAGAA AGCTCTCCAC TCTGAAAGAT GAAAGTTCTC 1141 GGGCCTGGAC AGAATCTAGT ATTAATACAT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 1201 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1261 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 1321 TGTGACAAAT GAAATGGTGG TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1381 ttgtgaaaga tt