Construct: ORF TRCN0000488194
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021145.1_s317c1
- DNA Barcode:
- AAGTGTTCCGTGAAACAATGACGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R60 (338398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488194
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338398 | TAS2R60 | taste 2 receptor member 60 | NM_177437.1 | 99.8% | 100% | 930T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1026
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatgga gaccacatgg ttctaggatc ttcggtgact gacaagaagg 121 ccatcatctt ggttaccatt ttactccttt tacgcctggt agcaatagca ggcaatggct 181 tcatcactgc tgctctgggc gtggagtggg tgctacggag aatgttgttg ccttgtgata 241 agttattggt tagcctaggg gcctctcgct tctgtctgca gtcagtggta atgggtaaga 301 ccatttatgt tttcttgcat ccgatggcct tcccatacaa ccctgtactg cagtttctag 361 ctttccagtg ggacttcctg aatgctgcca ccttatggtc ctctacctgg ctcagtgtct 421 tctattgtgt gaaaattgct accttcaccc accctgtctt cttctggcta aagcacaagt 481 tgtctgggtg gctaccatgg atgctcttca gctctgtagg gctctccagc ttcaccacca 541 ttctattttt cataggcaac cacagaatgt atcagaacta tttaaggaac catctacaac 601 cttggaatgt cactggcgat agcatacgga gctactgtga gaaattctat ctcttccctc 661 taaaaatgat tacttggaca atgcccactg ctgtcttttt catttgcatg attttgctca 721 tcacatctct GGGAAGACAC AGGAAGAAGG CTCTCCTTAC AACCTCAGGA TTCCGAGAGC 781 CCAGTGTGCA GGCACACATA AAGGCTCTGC TGGCTCTCCT CTCTTTTGCC ATGCTCTTCA 841 TCTCATATTT CCTGTCACTG GTGTTCAGTG CTGCAGGTAT TTTTCCACCT CTGGACTTTA 901 AATTCTGGGT GTGGGAGTCA GTGATTTATC TGTGTGCAGC AGTTCACCCC ATCATTCTGC 961 TCTTCAGCAA CTGCAGGCTG AGAGCTGTGC TGAAGAGTCG CCGTTCCTCA AGGTGTGGGA 1021 CACCTTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGTGT TCCGTGAAAC AATGACGAAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt