Construct: ORF TRCN0000488214
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021901.1_s317c1
- DNA Barcode:
- CAGCTGTCGACTTGTGAAGATCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- VIP (7432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488214
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7432 | VIP | vasoactive intestinal peptide | NM_003381.4 | 100% | 100% | |
| 2 | human | 7432 | VIP | vasoactive intestinal peptide | NM_194435.3 | 99.4% | 99.4% | 333_334insAGC |
| 3 | human | 7432 | VIP | vasoactive intestinal peptide | XM_006715562.4 | 99.4% | 99.4% | 465_466insAGC |
| 4 | human | 7432 | VIP | vasoactive intestinal peptide | XM_005267135.3 | 98.8% | 98.8% | 333_334insAGC;462_463insAGC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 582
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacacc agaaataagg cccagctcct tgtgctcctg actcttctca 121 gtgtgctctt ctcacagact tcggcatggc ctctttacag ggcaccttct gctctcaggt 181 tgggtgacag aatacccttt gagggagcaa atgaacctga tcaagtttca ttaaaagaag 241 acattgacat gttgcaaaat gcattagctg aaaatgacac acccTATTAT GATGTATCCA 301 GAAATGCCAG GCATGCTGAT GGAGTTTTCA CCAGTGACTT CAGTAAACTC TTGGGTCAAC 361 TTTCTGCCAA AAAGTACCTT GAGTCTCTTA TGGGAAAACG TGTTAGCAGT AACATCTCAG 421 AAGACCCTGT ACCAGTCAAA CGTCACTCAG ATGCAGTCTT CACTGACAAC TATACCCGCC 481 TTAGAAAACA AATGGCTGTA AAGAAATATT TGAACTCAAT TCTGAATGGA AAGAGGAGCA 541 GTGAGGGAGA ATCTCCCGAC TTTCCAGAAG AGTTAGAAAA ATGAGACCCA GCTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA CAGCTGTCGA CTTGTGAAGA TCCGACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt