Construct: ORF TRCN0000488227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020828.1_s317c1
- DNA Barcode:
- CGAGGAACTTGGTTTTAACTCCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR52 (9293)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488227
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9293 | GPR52 | G protein-coupled receptor 52 | NM_005684.5 | 99.9% | 100% | 105T>C |
| 2 | mouse | 620246 | Gpr52 | G protein-coupled receptor 52 | NM_001146330.1 | 91.4% | 95.5% | (many diffs) |
| 3 | mouse | 620246 | Gpr52 | G protein-coupled receptor 52 | XM_017321886.1 | 91.4% | 95.5% | (many diffs) |
| 4 | mouse | 620246 | Gpr52 | G protein-coupled receptor 52 | XM_017321888.1 | 91.4% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1155
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatgaa tccaggtgga ctgaatggag gatcctgaac atgagcagtg 121 gcattgtgaa tgtgtccgag cgtcactcct gcccacttgg atttggccac tacagcgtgg 181 tggatgtctg catcttcgag acagtggtta ttgtgttgct gacatttctg atcattgctg 241 ggaatctaac agttatcttt gtctttcatt gtgctccact gttacatcat tatactacca 301 gctatttcat tcagacgatg gcatatgctg atcttttcgt tggagttagc tgcttggttc 361 ctactctgtc acttctccac tactccacag gtgtccacga gtcattgact tgccaggttt 421 ttggatatat catctcagtt ctaaaaagtg tttctatggc atgtcttgct tgcatcagtg 481 tggatcgtta tcttgcaata accaagcctc tttcctacaa tcaactggtc accccttgtc 541 gcttgagaat ttgcattatt ttgatctgga tctactcctg cctaattttc ttgccttcct 601 tttttggctg ggggaaacct ggttaccatg gtgacatttt tgaatggtgt gccacgtctt 661 ggctcaccag tgcctatttt actggcttta ttgtttgttt actttatgct cctgctgcct 721 ttgttgtctg cttcacttac ttccacattt tcaaaatttg ccgtcagcac accaaagaga 781 taaatgaccg aagagcccga ttccctagtc atgaggtaga ttcttccaga gagactggac 841 acagccctga ccgtcgcTAC GCCATGGTTT TGTTTAGGAT AACCAGTGTA TTTTATATGC 901 TGTGGCTCCC CTATATAATT TACTTTCTTC TAGAAAGCTC CCGGGTCTTG GACAATCCAA 961 CTCTGTCCTT CTTAACAACC TGGCTTGCAA TAAGTAATAG TTTTTGTAAC TGTGTAATAT 1021 ACAGCCTCTC CAACAGCGTT TTCCGGCTAG GCCTCCGAAG ACTGTCTGAG ACAATGTGCA 1081 CATCCTGTAT GTGTGTGAAG GATCAGGAAG CACAAGAACC CAAACCTAGG AAACGGGCTA 1141 ATTCTTGCTC CATTTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGAGGAA CTTGGTTTTA 1321 ACTCCGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt