Construct: ORF TRCN0000488254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019637.1_s317c1
- DNA Barcode:
- CGTATGGTGGTGTCTAAAAGTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYRK3 (8444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488254
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8444 | DYRK3 | dual specificity tyrosine p... | NM_003582.3 | 100% | 100% | |
| 2 | human | 8444 | DYRK3 | dual specificity tyrosine p... | NM_001004023.2 | 95.6% | 95.5% | (many diffs) |
| 3 | human | 8444 | DYRK3 | dual specificity tyrosine p... | XM_005273315.4 | 95.6% | 95.5% | (many diffs) |
| 4 | human | 8444 | DYRK3 | dual specificity tyrosine p... | XM_011510061.2 | 94% | 94% | 0_1ins105 |
| 5 | mouse | 226419 | Dyrk3 | dual-specificity tyrosine-(... | NM_145508.2 | 84.8% | 89.6% | (many diffs) |
| 6 | mouse | 226419 | Dyrk3 | dual-specificity tyrosine-(... | XM_006529427.2 | 79.8% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 1854
- ORF length:
- 1764
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tcccaggaga tgggaggcac agctcgtggg cctgggcgga 121 aggatgcggg gccgcctggg gccgggctcc cgccccagca gcggaggttg ggggatggtg 181 tctatgacac cttcatgatg atagatgaaa ccaaatgtcc cccctgttca aatgtactct 241 gcaatccttc tgaaccacct ccacccagaa gactaaatat gaccactgag cagtttacag 301 gagatcatac tcagcacttt ttggatggag gtgagatgaa ggtagaacag ctgtttcaag 361 aatttggcaa cagaaaatcc aatactattc agtcagatgg catcagtgac tctgaaaaat 421 gctctcctac tgtttctcag ggtaaaagtt cagattgctt gaatacagta aaatccaaca 481 gttcatccaa ggcacccaaa gtggtgcctc tgactccaga acaagccctg aagcaatata 541 aacaccacct cactgcctat gagaaactgg aaataattaa ttatccagaa atttactttg 601 taggtccaaa tgccaagaaa agacatggag ttattggtgg tcccaataat ggagggtatg 661 atgatgcaga tggggcctat attcatgtac ctcgagacca tctagcttat cgatatgagg 721 tgctgaaaat tattggcaag gggagttttg ggcaggtggc cagggtctat gatcacaaac 781 ttcgacagta cgtggcccta aaaatggtgc gcaatgagaa gcgctttcat cgtcaagcag 841 ctgaggagat ccggattttg gagcatctta agaaacagga taaaactggt agtatgaacg 901 ttatccacat gctggaaagt ttcacattcc ggaaccatgt ttgcatggcc tttgaattgc 961 tgagcataga cctttatgag ctgattaaaa aaaataagtt tcagggtttt agcgtccagt 1021 tggtacgcaa gtttgcccag tccatcttgc aatctttgga tgccctccac aaaaataaga 1081 ttattcactg cgatctgaag ccagaaaaca ttctcctgaa acaccacggg cgcagttcaa 1141 ccaaggtcat tgactttggg tccagctgtt tcgagtacca gaagctctac acatatatcc 1201 agtctcggtt ctacagagct ccagaaatca tcttaggaag ccgctacagc acaccaattg 1261 acatatggag ttttggctgc atccttgcag aacttttaac aggacagcct ctcttccctg 1321 gagaggatga aggagaccag ttggcctgca tgatggagct tctagggatg ccaccaccaa 1381 aacttctgga gcaatccaaa cgtgccaagt actttattaa ttccaagggc ataccccgct 1441 actgctctgt gactacccag gcagatggga gggttgtgct tgtggggggt cgctcacgta 1501 ggggtaaaaa gcggggTCCC CCAGGCAGCA AAGACTGGGG GACAGCACTG AAAGGGTGTG 1561 ATGACTACTT GTTTATAGAG TTCTTGAAAA GGTGTCTTCA CTGGGACCCC TCTGCCCGCT 1621 TGACCCCAGC TCAAGCATTA AGACACCCTT GGATTAGCAA GTCTGTCCCC AGACCTCTCA 1681 CCACCATAGA CAAGGTGTCA GGGAAACGGG TAGTTAATCC TGCAAGTGCT TTCCAGGGAT 1741 TGGGTTCTAA GCTGCCTCCA GTTGTTGGAA TAGCCAATAA GCTTAAAGCT AACTTAATGT 1801 CAGAAACCAA TGGTAGTATA CCCCTATGCA GTGTATTGCC AAAACTGATT AGCAAGGGTG 1861 GGCGCGCCGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1921 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1981 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGTATG GTGGTGTCTA AAAGTCGAAC 2041 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt