Construct: ORF TRCN0000488261
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019339.1_s317c1
- DNA Barcode:
- CCACGCTGCTAGGGAAGCCTGGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR182 (11318)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488261
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11318 | GPR182 | G protein-coupled receptor 182 | NM_007264.4 | 99.9% | 99.7% | 1212_1213insG |
| 2 | human | 11318 | GPR182 | G protein-coupled receptor 182 | XM_011537824.3 | 99.9% | 99.7% | 1212_1213insG |
| 3 | human | 11318 | GPR182 | G protein-coupled receptor 182 | XM_011537825.3 | 99.9% | 99.7% | 1212_1213insG |
| 4 | human | 11318 | GPR182 | G protein-coupled receptor 182 | XM_011537827.3 | 99.9% | 99.7% | 1212_1213insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1290
- ORF length:
- 1215
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtca gtgaaaccca gctgggggcc tggcccctcg gagggggtca 121 ccgcagtgcc taccagtgac cttggagaga tccacaactg gaccgagctg cttgacctct 181 tcaaccacac tttgtctgag tgccacgtgg agctcagcca gagcaccaag cgcgtggtcc 241 tctttgccct ctacctggcc atgtttgtgg ttgggctggt ggagaacctc ctggtgatat 301 gcgtcaactg gcgcggctca ggccgggcag ggctgatgaa cctctacatc ctcaacatgg 361 ccatcgcgga cctgggcatt gtcctgtctc tgcccgtgtg gatgctggag gtcacgctgg 421 actacacctg gctctggggc agcttctcct gccgcttcac tcactacttc tactttgtca 481 acatgtatag cagcatcttc ttcctggtgt gcctcagtgt cgaccgctat gtcaccctca 541 ccagcgcctc cccctcctgg cagcgttacc agcaccgagt gcggcgggcc atgtgtgcag 601 gcatctgggt cctctcggcc atcatcccgc tgcctgaggt ggtccacatc cagctggtgg 661 agggccctga gcccatgtgc ctcttcatgg caccttttga aacgtacagc acctgggccc 721 tggcggtggc cctgtccacc accatcctgg gcttcctgct gcccttccct ctcatcacag 781 tcttcaatgt gctgacagcc tgccggctgc ggcagccagg acaacccaag agccggcgcc 841 actgcctgct gctgtgcgcc tacgtggccg tctttgtcat gtgctggctg ccctatcatg 901 tgaccctgct gctgctcaca ctgcatggga cccacatctc cctccactgc cacctggtcc 961 accTGCTCTA CTTCTTCTAT GATGTCATTG ACTGCTTCTC CATGCTGCAC TGTGTCATCA 1021 ACCCCATCCT TTACAACTTT CTCAGCCCAC ACTTCCGGGG CCGGCTCCTG AATGCTGTAG 1081 TCCATTACCT TCCTAAGGAC CAGACCAAGG CGGGCACATG CGCCTCCTCT TCCTCCTGTT 1141 CCACCCAGCA TTCCATCATC ATCACCAAGG GTGATAGCCA GCCTGCTGCA GCAGCCCCCC 1201 ACCCTGAGCC AAGCCTGAGC TTTCAGGCAC ACCATTTGCT TCCAAATACT TCCCCCATCT 1261 CTCCCACTCA GCCTCTTACA CCCAGCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCACGCTG 1441 CTAGGGAAGC CTGGGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt