Construct: ORF TRCN0000488266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021605.1_s317c1
- DNA Barcode:
- GAAGAGTGTCCTCTGGCGGATACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SRD5A1 (6715)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001047.4 | 99.8% | 100% | 90C>G |
| 2 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001324322.2 | 75.8% | 48.4% | 1_26del;116C>G;318_319ins167 |
| 3 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001324323.2 | 67.6% | 63.7% | (many diffs) |
| 4 | human | 6719 | SRD5A1P1 | steroid 5 alpha-reductase 1... | NR_028597.1 | 34.3% | (many diffs) | |
| 5 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NR_136739.2 | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 846
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcaacggcg acgggggtgg cggaggagcg cctgctggcc gcgctcgcct 121 acctgcagtg cgccgtgggc tgcgcggtct tcgcgcggaa tcgtcagacg aactcagtgt 181 acggccgcca cgcgctgccc agccacaggc tccgagtgcc ggcgcgggcc gcctgggtgg 241 tgcaggagct gccctcgctg gccctgccgc tctaccagta cgccagcgag tccgccccgc 301 gtctccgcag cgcgcccaac tgcatcctcc tggccatgtt cctcgtccac tacgggcatc 361 ggtgcttaat ttacccattt ctgatgcgag gaggaaagcc tatgccactg ttggcgtgta 421 caatggcgat tatgttctgt acctgtaacg gctatttgca aagcagatac ttgagccatt 481 gtgcagtgta tgctgatgac tgggtaacag atccccgttt tctaataggt tttggcttgt 541 ggttaacggg catgttgata aacatccatt cagatcatat ccTAAGGAAT CTCAGAAAAC 601 CAGGAGATAC TGGATACAAA ATACCAAGGG GAGGCTTATT TGAATACGTA ACTGCAGCCA 661 ACTATTTTGG AGAAATCATG GAGTGGTGTG GCTATGCCCT GGCCAGCTGG TCTGTCCAAG 721 GCGCGGCTTT TGCTTTCTTC ACGTTTTGTT TTTTATCTGG TAGAGCAAAA GAGCATCATG 781 AGTGGTACCT CCGGAAATTT GAAGAGTATC CAAAGTTCAG AAAAATTATA ATTCCATTTT 841 TGTTTTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGAAGAG TGTCCTCTGG CGGATACAAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt