Construct: ORF TRCN0000488273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021008.1_s317c1
- DNA Barcode:
- GATCGTCTGGAGCATGTGCGTGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DRD2 (1813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1813 | DRD2 | dopamine receptor D2 | NM_000795.4 | 100% | 100% | |
| 2 | human | 1813 | DRD2 | dopamine receptor D2 | XM_017017296.2 | 100% | 100% | |
| 3 | human | 1813 | DRD2 | dopamine receptor D2 | NM_016574.3 | 93.4% | 93.4% | 722_723ins87 |
| 4 | mouse | 13489 | Drd2 | dopamine receptor D2 | NM_010077.2 | 90.6% | 95.4% | (many diffs) |
| 5 | mouse | 13489 | Drd2 | dopamine receptor D2 | XM_006509996.3 | 84.3% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1401
- ORF length:
- 1329
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatcca ctgaatctgt cctggtatga tgatgatctg gagaggcaga 121 actggagccg gcccttcaac gggtcagacg ggaaggcgga cagaccccac tacaactact 181 atgccacact gctcaccctg ctcatcgctg tcatcgtctt cggcaacgtg ctggtgtgca 241 tggctgtgtc ccgcgagaag gcgctgcaga ccaccaccaa ctacctgatc gtcagcctcg 301 cagtggccga cctcctcgtc gccacactgg tcatgccctg ggttgtctac ctggaggtgg 361 taggtgagtg gaaattcagc aggattcact gtgacatctt cgtcactctg gacgtcatga 421 tgtgcacggc gagcatcctg aacttgtgtg ccatcagcat cgacaggtac acagctgtgg 481 ccatgcccat gctgtacaat acgcgctaca gctccaagcg ccgggtcacc gtcatgatct 541 ccatcgtctg ggtcctgtcc ttcaccatct cctgcccact cctcttcgga ctcaataacg 601 cagaccagaa cgagtgcatc attgccaacc cggccttcgt ggtctactcc tccatcgtct 661 ccttctacgt gcccttcatt gtcaccctgc tggtctacat caagatctac attgtcctcc 721 gcagacgccg caagcgagtc aacaccaaac gcagcagccg agctttcagg gcccacctga 781 gggctccact aaagggcaac tgtactcacc ccgaggacat gaaactctgc accgttatca 841 tgaagtctaa tgggagtttc ccagtgaaca ggcggagagt ggaggctgcc cggcgagccc 901 aggagctgga gatggagatg ctctccagca ccagcccacc cgagaggacc cggtacagcc 961 ccatcccacc cagccaccac cagctgactc tccccgaccc gtcccaccat ggtctccaca 1021 gcactcccga cagccccgcc aaaccagaga agaatgggca tgccaaagac caccccaaga 1081 ttgccaagat ctttgagatc cagaccatgc ccaatggcaa aacccggacc TCCCTCAAGA 1141 CCATGAGCCG TAGGAAGCTC TCCCAGCAGA AGGAGAAGAA AGCCACTCAG ATGCTCGCCA 1201 TTGTTCTCGG CGTGTTCATC ATCTGCTGGC TGCCCTTCTT CATCACACAC ATCCTGAACA 1261 TACACTGTGA CTGCAACATC CCGCCTGTCC TGTACAGCGC CTTCACGTGG CTGGGCTATG 1321 TCAACAGCGC CGTGAACCCC ATCATCTACA CCACCTTCAA CATTGAGTTC CGCAAGGCCT 1381 TCCTGAAGAT CCTCCACTGC TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG ATCGTCTGGA 1561 GCATGTGCGT GATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att