Construct: ORF TRCN0000488277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020961.1_s317c1
- DNA Barcode:
- CCGAGTTCGCCTCCTATACGTGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OR52B2 (255725)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488277
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255725 | OR52B2 | olfactory receptor family 5... | NM_001004052.1 | 100% | 100% | |
| 2 | mouse | 259063 | Olfr691 | olfactory receptor 691 | NM_147061.1 | 84.2% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1041
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagtcac accaatgtta ccatcttcca tcctgcagtt tttgtccttc 121 ctggcatccc tgggttggag gcttatcaca tttggctgtc aatacctctt tgcctcattt 181 acatcactgc agtcctggga aacagcatcc tgatagtggt tattgtcatg gaacgtaacc 241 ttcatgtgcc catgtatttc ttcctctcaa tgctggccgt catggacatc ctgctgtcta 301 ccaccactgt gcccaaggcc ctagccatct tttggcttca agcacataac attgcttttg 361 atgcctgtgt cacccaaggc ttctttgtcc atatgatgtt tgtgggggag tcagctatcc 421 tgttagccat ggcctttgat cgctttgtgg ccatttgtgc cccactgaga tatacaacag 481 tgctaacatg gcctgttgtg gggaggattg ctctggccgt catcacccga agcttctgca 541 tcatcttccc agtcatattc ttgctgaagc ggctgccctt ctgcctaacc aacattgttc 601 ctcactccta ctgtgagcat attggagtgg ctcgtttagc ctgtgctgac atcactgtta 661 acatttggta tggcttctca gtgcccattg tcatggtcat cttggatgtt atcctcatcg 721 ctgtgtctta ctcactgatc cTCCGAGCAG TGTTTCGTTT GCCCTCCCAG GATGCTCGGC 781 ACAAGGCCCT CAGCACTTGT GGCTCCCACC TCTGTGTCAT CCTTATGTTT TATGTTCCAT 841 CCTTCTTTAC CTTATTGACC CATCATTTTG GGCGTAATAT TCCTCAACAT GTCCATATCT 901 TGCTGGCCAA TCTTTATGTG GCAGTGCCAC CAATGCTGAA CCCCATTGTC TATGGTGTGA 961 AGACTAAGCA GATACGTGAG GGTGTAGCCC ACCGGTTCTT TGACATCAAG ACTTGGTGCT 1021 GTACCTCCCC TCTGGGCTCA TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CGAGTTCGCC 1201 TCCTATACGT GTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att