Construct: ORF TRCN0000488287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019205.1_s317c1
- DNA Barcode:
- GCTGGCTCTCGGAGGGTTAACGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR149 (344758)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488287
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 344758 | GPR149 | G protein-coupled receptor 149 | NM_001038705.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2268
- ORF length:
- 2193
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtct ttatttctca gtaacttatc aacaaatgac tctagcctgt 121 ggaaagagaa tcataattct acggaccttt taaatccgcc aggaaccctg aatatctatc 181 ttttttgctt gacatgtctc atgacttttg cagccttggt gggcagcatt tattcactaa 241 tttccctgct gaaaatgcag aacagaactg ttgtgtccat gcttgtggct tcctggtctg 301 tggatgatct catgagcgtc ctgtcggtga ccatcttcat gtttttgcag tggccaaacg 361 aggtccccgg ttacttccaa tttctgtgca ccacctctgc cttaatgtat ttatgccagg 421 gcctctctag caacttgaag gcgactctcc tagtctctta caacttttat acgatgcaca 481 gaggtgtggg gagccagaca gcctccagaa gatcgggcca ggtgctcggc gtggtgctga 541 ccgtgtgggc agccagtctg ctgctctcgg cgctcccgct gtgcggctgg ggcgccttcg 601 tgcgcacgcc ctggggctgc ctggtggact gctccagctc ctacgtacta ttcctctcta 661 tcgtgtacgc tttggccttc ggactcctcg tgggcctctc agtcccactc actcaccgat 721 tgctgtgttc ggaggagccg ccgagactcc actccaacta ccaggaaatt tcccgtggag 781 cttcaattcc tgggacccct cctactgcgg ggagagtggt ttccctgtcc ccagaggatg 841 ctccaggccc gagtctgcgg cgctctgggg gatgctctcc gagctccgac accgtgttcg 901 gaccgggtgc gcccgctgcc gctggggctg aagcctgcag gcgtgagaac cgggggactc 961 tctatggcac caggagcttc accgtgagcg tagcgcagaa gcgcttcgct ttgatcctag 1021 cgcttacaaa agtcgtcctt tggctgccca tgatgatgca catggtggtc cagaacgtcg 1081 tggggtttca gagccttccc ttggagacat tcagctttct acttaccctg ctggccacca 1141 ctgtaacccc agtgtttgtc ttgtccaaac gctggaccca cttgccctgt ggctgcatca 1201 tcaactgcag gcagaacgca tatgcagtgg cgtccgatgg gaaaaaaatc aagagaaaag 1261 gctttgaatt caatctatca ttccaaaaaa gttatgggat ttataaaata gcacatgaag 1321 attactatga tgatgatgaa aattccatat tctatcacaa cctgatgaac tctgagtgtg 1381 aaactacaaa agaccctcag agagacaacc gtaacatctt caatgctata aaagtagaaa 1441 tcagcaccac gccctctctg gacagctcca cacaaagagg catcaacaaa tgcacaaata 1501 ctgatattac agaagctaaa caggattcca acaacaaaaa ggatgcgttt tctgacaaaa 1561 caggaggtga tattaactat gaagaaacta ccttttctga agggccagaa agaagactgt 1621 ctcatgaaga gagtcagaaa ccagatcttt cagactggga gtggtgtagg agtaaatcag 1681 aaagaacccc tcgtcagcgt tccggttatg cccttgccat tcccttgtgt gcattccagg 1741 ggactgtgtc tctccatgca cctacaggga aaaccctatc tctttctacc tatgaggtaa 1801 gcgcagaagg gcaaaaaata actccagcct ctaagaaaat agaagtctat cgatccaaaa 1861 gtgttggcca tgaaccaaac tcagaagatt cttcatccac gtttgtggac accagtgtga 1921 aaatacactt ggaggttctt gaaatttgtg ataatgaaga ggccttggac actgtgtcaa 1981 tcattagtaa catcagtcag tcctccacac aagtcagatc tccatcccTA CGTTACTCCA 2041 GGAAAGAAAA CAGATTTGTT TCATGTGACC TAGGGGAAAC AGCCTCATAC TCCCTCTTTT 2101 TGCCCACCAG TAATCCTGAT GGTGATATTA ATATCTCCAT TCCAGACACA GTAGAAGCAC 2161 ACAGGCAGAA CAGTAAAAGG CAGCATCAAG AGAGGGATGG CTACCAGGAG GAAATCCAGT 2221 TGTTAAATAA AGCTTACAGA AAAAGAGAGG AAGAAAGCAA GGGTAGTGAC CCAGCTTTCT 2281 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2341 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2401 GTGGAAAGGA CGAGCTGGCT CTCGGAGGGT TAACGTCACG CGTTAAGTCg acaatcaacc 2461 tctggattac aaaatttgtg aaagatt