Construct: ORF TRCN0000488287

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019205.1_s317c1
DNA Barcode:
GCTGGCTCTCGGAGGGTTAACGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GPR149 (344758)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488287
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 344758 GPR149 G protein-coupled receptor 149 NM_001038705.3 100% 100%
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
2268
ORF length:
2193
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgtct ttatttctca gtaacttatc aacaaatgac tctagcctgt
121ggaaagagaa tcataattct acggaccttt taaatccgcc aggaaccctg aatatctatc
181ttttttgctt gacatgtctc atgacttttg cagccttggt gggcagcatt tattcactaa
241tttccctgct gaaaatgcag aacagaactg ttgtgtccat gcttgtggct tcctggtctg
301tggatgatct catgagcgtc ctgtcggtga ccatcttcat gtttttgcag tggccaaacg
361aggtccccgg ttacttccaa tttctgtgca ccacctctgc cttaatgtat ttatgccagg
421gcctctctag caacttgaag gcgactctcc tagtctctta caacttttat acgatgcaca
481gaggtgtggg gagccagaca gcctccagaa gatcgggcca ggtgctcggc gtggtgctga
541ccgtgtgggc agccagtctg ctgctctcgg cgctcccgct gtgcggctgg ggcgccttcg
601tgcgcacgcc ctggggctgc ctggtggact gctccagctc ctacgtacta ttcctctcta
661tcgtgtacgc tttggccttc ggactcctcg tgggcctctc agtcccactc actcaccgat
721tgctgtgttc ggaggagccg ccgagactcc actccaacta ccaggaaatt tcccgtggag
781cttcaattcc tgggacccct cctactgcgg ggagagtggt ttccctgtcc ccagaggatg
841ctccaggccc gagtctgcgg cgctctgggg gatgctctcc gagctccgac accgtgttcg
901gaccgggtgc gcccgctgcc gctggggctg aagcctgcag gcgtgagaac cgggggactc
961tctatggcac caggagcttc accgtgagcg tagcgcagaa gcgcttcgct ttgatcctag
1021cgcttacaaa agtcgtcctt tggctgccca tgatgatgca catggtggtc cagaacgtcg
1081tggggtttca gagccttccc ttggagacat tcagctttct acttaccctg ctggccacca
1141ctgtaacccc agtgtttgtc ttgtccaaac gctggaccca cttgccctgt ggctgcatca
1201tcaactgcag gcagaacgca tatgcagtgg cgtccgatgg gaaaaaaatc aagagaaaag
1261gctttgaatt caatctatca ttccaaaaaa gttatgggat ttataaaata gcacatgaag
1321attactatga tgatgatgaa aattccatat tctatcacaa cctgatgaac tctgagtgtg
1381aaactacaaa agaccctcag agagacaacc gtaacatctt caatgctata aaagtagaaa
1441tcagcaccac gccctctctg gacagctcca cacaaagagg catcaacaaa tgcacaaata
1501ctgatattac agaagctaaa caggattcca acaacaaaaa ggatgcgttt tctgacaaaa
1561caggaggtga tattaactat gaagaaacta ccttttctga agggccagaa agaagactgt
1621ctcatgaaga gagtcagaaa ccagatcttt cagactggga gtggtgtagg agtaaatcag
1681aaagaacccc tcgtcagcgt tccggttatg cccttgccat tcccttgtgt gcattccagg
1741ggactgtgtc tctccatgca cctacaggga aaaccctatc tctttctacc tatgaggtaa
1801gcgcagaagg gcaaaaaata actccagcct ctaagaaaat agaagtctat cgatccaaaa
1861gtgttggcca tgaaccaaac tcagaagatt cttcatccac gtttgtggac accagtgtga
1921aaatacactt ggaggttctt gaaatttgtg ataatgaaga ggccttggac actgtgtcaa
1981tcattagtaa catcagtcag tcctccacac aagtcagatc tccatcccTA CGTTACTCCA
2041GGAAAGAAAA CAGATTTGTT TCATGTGACC TAGGGGAAAC AGCCTCATAC TCCCTCTTTT
2101TGCCCACCAG TAATCCTGAT GGTGATATTA ATATCTCCAT TCCAGACACA GTAGAAGCAC
2161ACAGGCAGAA CAGTAAAAGG CAGCATCAAG AGAGGGATGG CTACCAGGAG GAAATCCAGT
2221TGTTAAATAA AGCTTACAGA AAAAGAGAGG AAGAAAGCAA GGGTAGTGAC CCAGCTTTCT
2281TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2341CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2401GTGGAAAGGA CGAGCTGGCT CTCGGAGGGT TAACGTCACG CGTTAAGTCg acaatcaacc
2461tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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