Construct: ORF TRCN0000488294
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019499.1_s317c1
- DNA Barcode:
- ACCGGTTCTCTACCCCTTATTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK11 (5600)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488294
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5600 | MAPK11 | mitogen-activated protein k... | NM_002751.7 | 99.8% | 100% | 507T>C;756A>G |
| 2 | human | 5600 | MAPK11 | mitogen-activated protein k... | NR_110887.2 | 72.6% | (many diffs) | |
| 3 | mouse | 19094 | Mapk11 | mitogen-activated protein k... | NM_011161.5 | 88.9% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 1185
- ORF length:
- 1092
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgaa ttcgctccgg acatgtcggg ccctcgcgcc ggcttctacc 121 ggcaggagct gaacaagacc gtgtgggagg tgccgcagcg gctgcagggg ctgcgcccgg 181 tgggctccgg cgcctacggc tccgtctgtt cggcctacga cgcccggctg cgccagaagg 241 tggcggtgaa gaagctgtcg cgccccttcc agtcgctgat ccacgcgcgc agaacgtacc 301 gggagctgcg gctgctcaag cacctgaagc acgagaacgt catcgggctt ctggacgtct 361 tcacgccggc cacgtccatc gaggacttca gcgaagtgta cttggtgacc accctgatgg 421 gcgccgacct gaacaacatc gtcaagtgcc aggcgctgag cgacgagcac gttcaattcc 481 tggtttacca gctgctgcgc gggctgaagt acatccactc ggccgggatc atccaccggg 541 acctgaagcc cagcaacgtg gctgtgaacg aggactgtga gctcaggatc ctggatttcg 601 ggctggcgcg ccaggcggac gaggagatga ccggctatgt ggccacgcgc tggtaccggg 661 cacctgagat catgctcaac tggatgcatt acaaccaaac agtggatatc tggtccgtgg 721 gctgcatcat ggctgagctg ctccagggca aggccctctt cccgggaagc gactacattg 781 accagctgaa gcgcatcatg gaagtggtgg gcacacccag ccctgaggtt ctggcaaaaa 841 tctcctcgga acacgcccgg acatatatcc agtccctgcc ccccatgccc cagaaggacc 901 TGAGCAGCAT CTTCCGTGGA GCCAACCCCC TGGCCATAGA CCTCCTTGGA AGGATGCTGG 961 TGCTGGACAG TGACCAGAGG GTCAGTGCAG CTGAGGCACT GGCCCACGCC TACTTCAGCC 1021 AGTACCACGA CCCCGAGGAT GAGCCAGAGG CCGAGCCATA TGATGAGAGC GTTGAGGCCA 1081 AGGAGCGCAC GCTGGAGGAG TGGAAGGAGC TCACTTACCA GGAAGTCCTC AGCTTCAAGC 1141 CCCCAGAGCC ACCGAAGCCA CCTGGCAGCC TGGAGATTGA GCAGAAGGGT GGGCGCGCCG 1201 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1261 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1321 TTTATATATC TTGTGGAAAG GACGAACCGG TTCTCTACCC CTTATTTTAA CGCGTTAAGT 1381 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt