Construct: ORF TRCN0000488302
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020394.1_s317c1
- DNA Barcode:
- AACGATAATTTATTCACATACAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STYK1 (55359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488302
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | NM_018423.3 | 99.9% | 100% | 366T>C |
| 2 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_005253417.2 | 99.9% | 100% | 366T>C |
| 3 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_011520736.1 | 99.9% | 100% | 366T>C |
| 4 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_011520737.2 | 99.9% | 100% | 366T>C |
| 5 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_011520738.2 | 99.9% | 100% | 366T>C |
| 6 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_017019579.1 | 68.7% | 68.7% | 0_1ins396 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1338
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcatg acacggatgc tcctggaatg cagtctcagt gacaagttgt 121 gtgtcatcca ggagaagcag tatgaagtga ttatcgtccc aactttgttg gttactatct 181 tcctcatcct tcttggggtc atcctgtggc tttttatcag agaacaaaga actcaacagc 241 agcgttctgg acctcaaggc attgcccctg ttcctccacc tagggaccta agctgggaag 301 caggacatgg aggaaatgtg gctttgccac ttaaggagac atccgtggaa aactttctgg 361 gagctaccac acctgccctg gctaagctgc aggtgccgcg ggagcaactc tctgaagttc 421 tggagcagat ttgcagcggt agctgtgggc ccatctttcg agccaatatg aacactgggg 481 acccttctaa gcccaagagt gttattctca aggctttaaa agaaccagct gggctccatg 541 aggtacaaga tttcttaggg cgaatccaat tccatcaata cctggggaaa cacaaaaacc 601 tggtgcagct ggaaggctgc tgcactgaaa agctgccact ctatatggtg ttggaggatg 661 tggcccaggg ggacctgctc agctttctct ggacctgtcg gcgggatgtg atgactatgg 721 atggtcttct ctatgatctc acagaaaaac aagtatatca catcggaaag caggtccttt 781 tggcgctgga attcctgcag gagaagcatt tgttccatgg ggatgtggca gccaggaata 841 ttctgatgca aagtgatctc actgctaagc tctgtggatt aggcctggct tatgaagttt 901 acacccgagg ggccatctcc tctactcaaa ccatacctct caagtggctt gccccagaac 961 ggcttctcct gagacctgct agcatcagag cagatgtctg gtcttttggg atccTGCTCT 1021 ATGAGATGGT GACTCTAGGA GCACCACCGT ATCCTGAAGT CCCTCCTACC AGCATCCTAG 1081 AGCATCTCCA AAGAAGGAAA ATCATGAAGA GACCCAGTAG CTGCACACAT ACCATGTACA 1141 GTATCATGAA GTCCTGCTGG CGCTGGCGTG AGGCTGACCG CCCCTCACCT AGAGAGCTGC 1201 GCTTGCGCCT AGAAGCTGCC ATTAAAACTG CAGATGACGA GGCTGTGTTA CAAGTACCAG 1261 AGTTGGTGGT ACCTGAACTG TATGCAGCTG TGGCCGGCAT CAGAGTGGAG AGCCTCTTCT 1321 ACAACTATAG CATGCTTTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACG ATAATTTATT 1501 CACATACAGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt