Construct: ORF TRCN0000488307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020051.1_s317c1
- DNA Barcode:
- GCCATACTTTACCACACTCAATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR37 (2861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488307
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2861 | GPR37 | G protein-coupled receptor 37 | NM_005302.5 | 99.8% | 99.8% | 1047T>C;1329G>C;1839_1840insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1914
- ORF length:
- 1842
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgagcc ccgggcgcgc ttctcgcccg catgtcgcgg ctactgcttc 121 tgctactgct caaggtgtct gcctcttctg ccctcggggt cgcccctgcg tccagaaacg 181 aaacttgtct gggggagagc tgtgcaccta cagtgatcca gcgccgcggc agggacgcct 241 ggggaccggg aaattctgca agagacgttc tgcgagcccg agcacccagg gaggagcagg 301 gggcagcgtt tcttgcggga ccctcctggg acctgccggc ggccccgggc cgtgacccgg 361 ctgcaggcag aggggcggag gcgtcggcag ccggaccccc gggacctcca accaggccac 421 ctggcccctg gaggtggaaa ggtgctcggg gtcaggagcc ttctgaaact ttggggagag 481 ggaaccccac ggccctccag ctcttccttc agatctcaga ggaggaagag aagggtccca 541 gaggcgctgg catttccggg cgtagccagg agcagagtgt gaagacagtc cccggagcca 601 gcgatctttt ttactggcca aggagagccg ggaaactcca gggttcccac cacaagcccc 661 tgtccaagac ggccaatgga ctggcggggc acgaagggtg gacaattgca ctcccgggcc 721 gggcgctggc ccagaatgga tccttgggtg aaggaatcca tgagcctggg ggtccccgcc 781 ggggaaacag cacgaaccgg cgtgtgagac tgaagaaccc cttctacccg ctgacccagg 841 agtcctatgg agcctacgcg gtcatgtgtc tgtccgtggt gatcttcggg accggcatca 901 ttggcaacct ggcggtgatg tgcatcgtgt gccacaacta ctacatgcgg agcatctcca 961 actccctctt ggccaacctg gccttctggg actttctcat catcttcttc tgccttccgc 1021 tggtcatctt ccacgagctg accaagaagt ggctgctgga ggacttctcc tgcaagatcg 1081 tgccctatat agaggtcgct tctctgggag tcaccacctt caccttatgt gctctgtgca 1141 tagaccgctt ccgtgctgcc accaacgtac agatgtacta cgaaatgatc gaaaactgtt 1201 cctcaacaac tgccaaactt gctgttatat gggtgggagc tctattgtta gcacttccag 1261 aagttgttct ccgccagctg agcaaggagg atttggggtt tagtggccga gctccggcag 1321 aaaggtgcat tattaagatc tctcctgatt taccagacac catctatgtt ctagccctca 1381 cctacgacag tgcgagactc tggtggtatt ttggctgtta cttttgtttg cccacgcttt 1441 tcaccatcac ctgctctcta gtgactgcga ggaaaatccg caaagcagag aaagcctgta 1501 cccgagggaa taaacggcag attcaactag agagtcagat gaactgtaca gtagtggcac 1561 tgaccatttt atatggattt tgcattattc ctgaaaatat ctgcaacatt gttactgcct 1621 acatggctac aggGGTTTCA CAGCAGACAA TGGACCTCCT TAATATCATC AGCCAGTTCC 1681 TTTTGTTCTT TAAGTCCTGT GTCACCCCAG TCCTCCTTTT CTGTCTCTGC AAACCCTTCA 1741 GTCGGGCCTT CATGGAGTGC TGCTGCTGTT GCTGTGAGGA ATGCATTCAG AAGTCTTCAA 1801 CGGTGACCAG TGATGACAAT GACAACGAGT ACACCACGGA ACTCGAACTC TCGCCTTTCA 1861 GTACCATACG CCGTGAAATG TCCACTTTTG CTTCTGTCGG AACTCATTGC GACCCAGCTT 1921 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1981 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2041 CTTGTGGAAA GGACGAGCCA TACTTTACCA CACTCAATGC ACGCGTTAAG TCgacaatca 2101 acctctggat tacaaaattt gtgaaagatt