Construct: ORF TRCN0000488311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019435.1_s317c1
- DNA Barcode:
- AGTTCCTGAGAGGACCTCAACATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P2RY1 (5028)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488311
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5028 | P2RY1 | purinergic receptor P2Y1 | NM_002563.5 | 99.8% | 99.7% | 786A>G;1119_1120insG |
| 2 | mouse | 18441 | P2ry1 | purinergic receptor P2Y, G-... | NM_001282016.1 | 87.1% | 94.6% | (many diffs) |
| 3 | mouse | 18441 | P2ry1 | purinergic receptor P2Y, G-... | NM_008772.5 | 87.1% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1191
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaccgaggtg ctgtggccgg ctgtccccaa cgggacggac gctgccttcc 121 tggccggtcc gggttcgtcc tgggggaaca gcacggtcgc ctccactgcc gccgtctcct 181 cgtcgttcaa atgcgccttg accaagacgg gcttccagtt ttactacctg ccggctgtct 241 acatcttggt attcatcatc ggcttcctgg gcaacagcgt ggccatctgg atgttcgtct 301 tccacatgaa gccctggagc ggcatctccg tgtacatgtt caatttggct ctggccgact 361 tcttgtacgt gctgactctg ccagccctga tcttctacta cttcaataaa acagactgga 421 tcttcgggga tgccatgtgt aaactgcaga ggttcatctt tcatgtgaac ctctatggca 481 gcatcttgtt tctgacatgc atcagtgccc accggtacag cggtgtggtg taccccctca 541 agtccctggg ccggctcaaa aagaagaatg cgatctgtat cagcgtgctg gtgtggctca 601 ttgtggtggt ggcgatctcc cccatcctct tctactcagg taccggggtc cgcaaaaaca 661 aaaccatcac ctgttacgac accacctcag acgagtacct gcgaagttat ttcatctaca 721 gcatgtgcac gaccgtggcc atgttctgtg tccccttggt gctgattctg ggctgttacg 781 gattaattgt gagagctttg atttacaaag atctggacaa ctctcctctg aggagaaaat 841 cgatttaccT GGTGATCATT GTACTGACTG TTTTTGCTGT GTCTTACATC CCTTTCCATG 901 TGATGAAAAC GATGAACTTG AGGGCCCGGC TTGATTTTCA GACCCCAGCA ATGTGTGCTT 961 TCAATGACAG GGTTTATGCC ACGTATCAGG TGACAAGAGG TCTAGCAAGT CTCAACAGTT 1021 GTGTGGACCC CATTCTCTAT TTCTTGGCGG GAGATACTTT CAGAAGGAGA CTCTCCCGAG 1081 CCACAAGGAA AGCTTCTAGA AGAAGTGAGG CAAATTTGCA ATCCAAGAGT GAAGACATGA 1141 CCCTCAATAT TTTACCTGAG TTCAAGCAGA ATGGAGATAC AAGCCTGGAC CCAGCTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGAAGTTCCT GAGAGGACCT CAACATCACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt