Construct: ORF TRCN0000488317
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021278.1_s317c1
- DNA Barcode:
- GCCGCATCTAATGATAAATTTTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RGR (5995)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488317
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5995 | RGR | retinal G protein coupled r... | XM_024448118.1 | 50.6% | 20.1% | (many diffs) |
| 2 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012722.1 | 42.5% | 17.1% | (many diffs) |
| 3 | human | 5995 | RGR | retinal G protein coupled r... | NM_002921.3 | 38.4% | 15.3% | (many diffs) |
| 4 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012720.2 | 37.1% | 14.9% | (many diffs) |
| 5 | human | 5995 | RGR | retinal G protein coupled r... | XR_002957005.1 | 11.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 432
- ORF length:
- 360
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcagag accagtgccT TGCCCACCGG CTTCGGGGAG CTCGAGGTGC 121 TGGCTGTGGG GATGGTGCTA CTGGTGGAAG ACCCCGGAGC TGCGGACTCC CTGCCACCTA 181 CTGGTGCTGA GCTTGGCTCT TGCGGACAGT GGGATCAGCC TGAATGCCCT CGTTGCAGCC 241 ACATCCAGCC TTCTCCGTGT CTCCCACAGG CGCTGGCCCT ACGGCTCGGA CGGCTGCCAG 301 GCTCACGGCT TCCAGGGCTT TGTGACAGCG TTGGCCAGCA TCTGCAGCAG TGCAGCCATC 361 GCATGGGGGC GTTATCACCA CTACTGCACC CGCCAAAATG CTGCCATCGG TCTCATCATT 421 TTGAGTGGCG ATAGAACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 481 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 541 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCCGCATCTA ATGATAAATT 601 TTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt