Construct: ORF TRCN0000488321
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020943.1_s317c1
- DNA Barcode:
- ACCGAGTAACGGCCCCCTTCCCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OR6B2 (389090)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488321
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389090 | OR6B2 | olfactory receptor family 6... | NM_001005853.1 | 100% | 100% | |
| 2 | human | 150681 | OR6B3 | olfactory receptor family 6... | NM_173351.1 | 90.9% | 89.1% | (many diffs) |
| 3 | human | 150681 | OR6B3 | olfactory receptor family 6... | XM_011510671.2 | 68.7% | 67.3% | (many diffs) |
| 4 | mouse | 259040 | Olfr1416 | olfactory receptor 1416 | NM_147038.1 | 86.3% | 85.8% | (many diffs) |
| 5 | mouse | 259041 | Olfr1414 | olfactory receptor 1414 | NM_147039.2 | 85.6% | 86.8% | (many diffs) |
| 6 | mouse | 258228 | Olfr1415 | olfactory receptor 1415 | NM_001011525.1 | 85.4% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1008
- ORF length:
- 936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagtggg gagaatgtca ccaaggtcag caccttcatc ctggtgggcc 121 tccccacggc cccagggctg cagtacctgc tcttcctcct cttcctgctc acctacctct 181 ttgtcctggt ggagaacctg gccatcatcc tcatcgtctg gagcagcacc tccctccaca 241 ggcccatgta ctactttctg agctccatgt ctttcctgga gatctggtac gtgtctgaca 301 tcacccccaa gatgctggag ggcttcctcc tccagcagaa acgcatctct ttcgtcgggt 361 gcatgacgca gctctacttc ttcagctccc tggtgtgcac cgagtgtgtg cttctggcct 421 ccatggccta cgaccgctac gtggccatct gccacccgct gcgctaccac gtccttgtga 481 ccccggggct gtgcctccag ctggtgggct tctcctttgt gagtggcttc accatctcca 541 tgatcaaggt ctgttttatc tccagcgtca cgttctgtgg ctccaacgtc ttgaaccact 601 tcttctgtga catttccccc atcctcaagc tggcctgcac ggacttctcc actgcagagc 661 tggtggattt catcctggcc ttcatcatcc tggtgttTCC GCTCCTGGCC ACCATACTGT 721 CATATTGGCA CATCACCCTG GCTGTCCTGC GCATCCCCTC GGCCACCGGC TGCTGGAGAG 781 CCTTCTCTAC CTGCGCCTCT CACCTCACCG TGGTCACCGT CTTCTATACA GCCTTGCTTT 841 TCATGTATGT CCGGCCCCAA GCCATTGATT CCCAGAGCTC CAACAAGCTC ATCTCTGCCG 901 TGTACACTGT TGTCACGCCA ATAATTAACC CTTTGATTTA CTGCCTGAGG AACAAGGAAT 961 TTAAGGACGC CTTGAAAAAG GCCTTGGGCT TGGGTCAAAC TTCACACTAG AACCCAGCTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGAACCG AGTAACGGCC CCCTTCCCAA ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt