Construct: ORF TRCN0000488326
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020556.1_s317c1
- DNA Barcode:
- AGTCAGCTAACGTAAGTCCACAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K13 (9175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488326
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_001242314.2 | 100% | 100% | |
| 2 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_004721.5 | 100% | 100% | |
| 3 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_011513310.2 | 100% | 100% | |
| 4 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007456.1 | 100% | 100% | |
| 5 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007457.1 | 84.3% | 83.4% | (many diffs) |
| 6 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_001242317.2 | 77.7% | 77.2% | (many diffs) |
| 7 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007458.1 | 75.9% | 75.9% | 0_1ins696 |
| 8 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007459.2 | 75.9% | 75.9% | 0_1ins696 |
| 9 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | NM_172821.3 | 84.5% | 89% | (many diffs) |
| 10 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522582.3 | 84.5% | 89% | (many diffs) |
| 11 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522583.3 | 84.5% | 89% | (many diffs) |
| 12 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522584.3 | 84.5% | 89% | (many diffs) |
| 13 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_011246015.2 | 84.5% | 89% | (many diffs) |
| 14 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_011246016.2 | 84.5% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2970
- ORF length:
- 2898
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccaac tttcaggagc acctgagctg ctcctcttct ccacacttac 121 ccttcagtga aagcaaaacc ttcaatggac tacaagatga gctcacagct atggggaacc 181 acccttctcc caagctgctc gaggaccagc aggaaaaggg gatggtacga acagagctaa 241 tcgagagcgt gcacagcccc gtcaccacaa cagtgttgac gagcgtaagt gaggattcca 301 gggaccagtt tgagaacagc gttcttcagc taagggaaca cgatgaatca gagacggcgg 361 tgtctcaggg gaacagcaac acggtggacg gagagagcac aagcggaact gaagacataa 421 agattcagtt cagcaggtca ggcagtggca gtggtgggtt tcttgaagga ctatttggat 481 gcttaaggcc tgtatggaat atcattggga aggcatattc cactgattac aaattgcagc 541 agcaagatac ttgggaagtg ccatttgagg agatctcaga gctgcagtgg ctgggtagtg 601 gagcccaagg agcggtcttc ttgggcaagt tccgggcgga agaggtggcc atcaagaaag 661 tgagagaaca gaatgagacg gatatcaagc atttgaggaa gttgaagcac cctaacatca 721 tcgcattcaa gggtgtttgt actcaggccc catgttattg tattatcatg gaatactgtg 781 cccatggaca actctacgag gtcttacgag ctggcaggaa gatcacacct cgattgctag 841 tagactggtc cacaggaatt gcaagtggaa tgaattattt gcacctccat aaaattattc 901 atcgtgatct caaatcacct aatgttttag tgacccacac agatgcggta aaaatttcag 961 attttggtac atctaaggaa ctcagtgaca aaagtaccaa gatgtcattt gctggcacgg 1021 tcgcatggat ggcgccagag gtgatacgga atgaacctgt ctctgaaaaa gttgatatat 1081 ggtcttttgg agtggtgctt tgggagctgc tgacaggaga gatcccttac aaagatgtag 1141 attcttcagc cattatctgg ggtgttggaa gcaacagcct ccaccttcca gttccttcca 1201 cttgccctga tggattcaaa atccttatga aacagacgtg gcagagtaaa cctcgaaacc 1261 gaccttcttt tcggcagaca ctcatgcatt tagacattgc ctctgcagat gtacttgcca 1321 ccccacaaga aacttacttc aagtctcagg ctgaatggag agaagaagtg aaaaaacatt 1381 ttgagaagat caaaagtgaa ggaacttgta tacaccggtt agatgaagaa ctgattcgaa 1441 ggcgcagaga agagctcagg catgcgctgg atattcgtga acactatgag cggaagcttg 1501 agcgggcgaa taatttatac atggaattga gtgccatcat gctgcagcta gaaatgcggg 1561 agaaggagct cattaagcgt gagcaagcag tggaaaagaa gtatcctggg acctacaaac 1621 gacaccctgt tcgtcctatc atccatccca atgccatgga gaaactcatg aaaaggaaag 1681 gagtgcctca caaatctggg atgcagacca aacggccaga cttgttgaga tcagaaggga 1741 tccccaccac agaagtggct cccactgcat cccctttgtc cggaagtccc aaaatgtcca 1801 cttctagcag caagagccga tatcgaagca aaccacgcca ccgccgaggg aatagcagag 1861 gcagccatag tgactttgcc gcaatcttga aaaaccagcc agcccaggaa aattcacccc 1921 atcccactta cctgcaccaa gctcaatccc aatacccttc tcttcatcac cataattctc 1981 tgcagcagca ataccagcag ccccctcctg ccatgtccca gagtcaccat cccagactca 2041 atatgcacgg acaggacata gcaacctgcg ccaacaacct gaggtatttc ggcccagcag 2101 cagccctgcg gagcccactc agcaaccatg ctcagagaca gctgcccggc tcgagccctg 2161 acctcatctc cacagccatg gctgcagact gctggagaag ttctgagcct gacaagggcc 2221 aagctggtcc ctggggctgt tgccaggctg acgcttatga cccctgcctt cagtgcaggc 2281 cagaacagta tgggtcctta gacataccct ctgctgagcc agtggggagg agccctgacc 2341 tttccaagtc accagcacat aatcctctct tggaaaacgc ccagagttct gagaaaacgg 2401 aagaaaatga attcagcggc tgtaggtctg agtcatccct cggcacctct catctcggca 2461 cccctccagc gctacctcga aaaacaaggc ctctgcagaa gagtggagat gactcctcag 2521 aagaggaaga aggggaagta gatagtgaag ttgaatttcc acgaagacag aggccccatc 2581 gctgtatcag cagctgccag tcatattcaa cctttagctc tgagaatttc tctgtgtctg 2641 atGGAGAAGA GGGAAATACC AGTGACCACT CAAACAGTCC TGATGAGTTA GCTGATAAAC 2701 TTGAAGACCG CTTGGCAGAG AAGCTAGACG ACCTGCTGTC CCAGACGCCA GAGATTCCCA 2761 TTGACATATC CTCACACTCG GATGGGCTCT CTGACAAGGA GTGTGCCGTG CGCCGTGTGA 2821 AGACTCAGAT GTCTCTGGGC AAGCTGTGTG TGGAGGAACG TGGCTATGAG AACCCCATGC 2881 AGTTTGAAGA ATCGGACTGT GACTCTTCAG ATGGGGAGTG TTCTGATGCC ACAGTTAGGA 2941 CCAATAAACA CTACAGCTCT GCTACCTGGT AAGCTAGCGA CCCAGCTTTC TTGTACAAAG 3001 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3061 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3121 ACGAAGTCAG CTAACGTAAG TCCACAGCAC GCGTTAAGTC gacaatcaac ctctggatta 3181 caaaatttgt gaaagatt