Construct: ORF TRCN0000488339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019307.1_s317c1
- DNA Barcode:
- GCTGTTAGTAGAAACTGTTGTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR101 (83550)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83550 | GPR101 | G protein-coupled receptor 101 | NM_054021.1 | 99.8% | 99.6% | 370G>T;1524_1525insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1602
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgacg tccacctgca ccaacagcac gcgcgagagt aacagcagcc 121 acacgtgcat gcccctctcc aaaatgccca tcagcctggc ccacggcatc atccgctcaa 181 ccgtgctggt tatcttcctc gccgcctctt tcgtcggcaa catagtgctg gcgctagtgt 241 tgcagcgcaa gccgcagctg ctgcaggtga ccaaccgttt tatctttaac ctcctcgtca 301 ccgacctgct gcagatttcg ctcgtggccc cctgggtggt ggccacctct gtgcctctct 361 tctggcccct caacagccac ttctgcacgg ccctggttag cctcacccac ctgttcgcct 421 tcgccagcgt caacaccatt gtcttggtgt cagtggatcg ctacttgtcc atcatccacc 481 ctctctccta cccgtccaag atgacccagc gccgcggtta cctgctcctc tatggcacct 541 ggattgtggc catcctgcag agcactcctc cactctacgg ctggggccag gctgcctttg 601 atgagcgcaa tgctctctgc tccatgatct ggggggccag ccccagctac actattctca 661 gcgtggtgtc cttcatcgtc attccactga ttgtcatgat tgcctgctac tccgtggtgt 721 tctgtgcagc ccggaggcag catgctctgc tgtacaatgt caagagacac agcttggaag 781 tgcgagtcaa ggactgtgtg gagaatgagg atgaagaggg agcagagaag aaggaggagt 841 tccaggatga gagtgagttt cgccgccagc atgaaggtga ggtcaaggcc aaggagggca 901 gaatggaagc caaggacggc agcctgaagg ccaaggaagg aagcacgggg accagtgaga 961 gtagtgtaga ggccaggggc agcgaggagg tcagagagag cagcacggtg gccagcgacg 1021 gcagcatgga gggtaaggaa ggcagcacca aagttgagga gaacagcatg aaggcagaca 1081 agggtcgcac agaggtcaac cagtgcagca ttgacttggg tgaagatgac atggagtttg 1141 gtgaagacga catcaatttc agtgaggatg acgtcgaggc agtgaacatc ccggagagcc 1201 tcccacccag tcgtcgtaac agcaacagca accctcctct gcccaggtgc taccagtgca 1261 aagctgctaa agtgatcttc atcatcattt tctcctatgt gctatcCCTG GGGCCCTACT 1321 GCTTTTTAGC AGTCCTGGCC GTGTGGGTGG ATGTCGAAAC CCAGGTACCC CAGTGGGTGA 1381 TCACCATAAT CATCTGGCTT TTCTTCCTGC AGTGCTGCAT CCACCCCTAT GTCTATGGCT 1441 ACATGCACAA GACCATTAAG AAGGAAATCC AGGACATGCT GAAGAAGTTC TTCTGCAAGG 1501 AAAAGCCCCC GAAAGAAGAT AGCCACCCAG ACCTGCCCGG AACAGAGGGT GGGACTGAAG 1561 GCAAGATTGT CCCTTCCTAC GATTCTGCTA CTTTTCCTGA CCCAGCTTTC TTGTACAAAG 1621 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1681 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1741 ACGAGCTGTT AGTAGAAACT GTTGTCGGAC GCGTTAAGTC gacaatcaac ctctggatta 1801 caaaatttgt gaaagatt