Construct: ORF TRCN0000488352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020836.1_s317c1
- DNA Barcode:
- CGAGTGTACAGGTAAAGCGCTCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NMUR2 (56923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56923 | NMUR2 | neuromedin U receptor 2 | NM_020167.4 | 99.8% | 99.5% | 611G>T;1183G>A |
| 2 | human | 56923 | NMUR2 | neuromedin U receptor 2 | XM_011537670.2 | 75.5% | 75.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1317
- ORF length:
- 1245
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcaggg atggaaaaac ttcagaatgc ttcctggatc taccagcaga 121 aactagaaga tccattccag aaacacctga acagcaccga ggagtatctg gccttcctct 181 gcggacctcg gcgcagccac ttcttcctcc ccgtgtctgt ggtgtatgtg ccaatttttg 241 tggtgggggt cattggcaat gtcctggtgt gcctggtgat tctgcagcac caggctatga 301 agacgcccac caactactac ctcttcagcc tggcggtctc tgacctcctg gtcctgctcc 361 ttggaatgcc cctggaggtc tatgagatgt ggcgcaacta ccctttcttg ttcgggcccg 421 tgggctgcta cttcaagacg gccctctttg agaccgtgtg cttcgcctcc atcctcagca 481 tcaccaccgt cagcgtggag cgctacgtgg ccatcctaca cccgttccgc gccaaactgc 541 agagcacccg gcgccgggcc ctcaggatcc tcggcatcgt ctggggcttc tccgtgctct 601 tctccctgcc caacaccagc atccatggca tcaagttcca ctacttcccc aatgggtccc 661 tggtcccagg ttcggccacc tttacggtca tcaagcccat gtggatctac aatttcatca 721 tccaggtcac ctccttccta ttctacctcc tccccatgac tgtcatcagt gtcctctact 781 acctcatggc actcagacta aagaaagaca aatctcttga ggcagatgaa gggaatgcaa 841 atattcaaag accctgcaga aaatcagtca acaagatgct gtttgtcttg gtcttagtgt 901 ttgctatctg ttgggccccg ttccacattg accgactctt cttcagcttt gtggaggagt 961 ggagtgaatc cctggctgct gtgttcaacc tcgtccatgt ggtgtcaggt gtcttcttct 1021 acctgagcTC AGCTGTCAAC CCCATTATCT ATAACCTACT GTCTCGCCGC TTCCAGGCAG 1081 CATTCCAGAA TGTGATCTCT TCTTTCCACA AACAGTGGCA CTCCCAGCAT GACCCACAGT 1141 TGCCACCTGC CCAGCGGAAC ATCTTCCTGA CAGAATGCCA CTTTGTGGAG CTGACCGAAG 1201 ATATAGGTCC CCAATTCCCA TGTCAGTCAT CCATGCACAA CTCTCACCTC CCAACAGCCC 1261 TCTCTAGTGA ACAGATGTCA AGAACAAACT ATCAAAGCTT CCACTTTAAC AAAACCTAGG 1321 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGACGAGT GTACAGGTAA AGCGCTCCAA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt