Construct: ORF TRCN0000488372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021085.1_s317c1
- DNA Barcode:
- CGGTCTATTACGCCGAACGGGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OPRD1 (4985)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4985 | OPRD1 | opioid receptor delta 1 | NM_000911.4 | 99.9% | 100% | 921C>T |
| 2 | mouse | 18386 | Oprd1 | opioid receptor, delta 1 | NM_013622.3 | 89% | 93.5% | (many diffs) |
| 3 | mouse | 18386 | Oprd1 | opioid receptor, delta 1 | XM_006538628.3 | 75.7% | 73% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1188
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaaccg gccccctccg ccggcgccga gctgcagccc ccgctcttcg 121 ccaacgcctc ggacgcctac cctagcgcct gccccagcgc tggcgccaat gcgtcggggc 181 cgccaggcgc gcggagcgcc tcgtccctcg ccctggcaat cgccatcacc gcgctctact 241 cggccgtgtg cgccgtgggg ctgctgggca acgtgcttgt catgttcggc atcgtccggt 301 acactaagat gaagacggcc accaacatct acatcttcaa cctggcctta gccgatgcgc 361 tggccaccag cacgctgcct ttccagagtg ccaagtacct gatggagacg tggcccttcg 421 gcgagctgct ctgcaaggct gtgctctcca tcgactacta caatatgttc accagcatct 481 tcacgctcac catgatgagt gttgaccgct acatcgctgt ctgccaccct gtcaaggccc 541 tggacttccg cacgcctgcc aaggccaagc tgatcaacat ctgtatctgg gtcctggcct 601 caggcgttgg cgtgcccatc atggtcatgg ctgtgacccg tccccgggac ggggcagtgg 661 tgtgcatgct ccagttcccc agccccagct ggtactggga cacggtgacc aagatctgcg 721 tgttcctctt cgccttcgtg gtgcccatcc tcatcatcac cgtgtgctat ggcctcatgc 781 tgctgcgcct gcgcagtgtg cgcctgctgt cgggctccaa ggagaaggac cgcagcctgc 841 ggcgcatcac gcgcatggtg ctggtggttg tgggcgccTT CGTGGTGTGT TGGGCGCCCA 901 TCCACATCTT CGTCATCGTC TGGACGCTGG TGGACATCGA CCGGCGCGAC CCGCTGGTGG 961 TGGCTGCGCT GCACCTGTGC ATCGCGCTGG GTTACGCCAA TAGCAGCCTC AACCCCGTGC 1021 TCTACGCTTT CCTCGACGAG AACTTCAAGC GCTGCTTCCG CCAGCTCTGC CGCAAGCCCT 1081 GCGGCCGCCC AGACCCCAGC AGCTTCAGCC GCGCCCGCGA AGCCACGGCC CGCGAGCGTG 1141 TCACCGCCTG CACCCCGTCC GATGGTCCCG GCGGTGGCGC TGCCGCCTGA GACCCAGCTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGACGGT CTATTACGCC GAACGGGTTC ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt