Construct: ORF TRCN0000488380
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020175.1_s317c1
- DNA Barcode:
- ATTTCAGTCGATCTCTCTACCCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TRAP1 (10131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488380
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10131 | TRAP1 | TNF receptor associated pro... | NM_016292.3 | 64.2% | 62.3% | 1314delA;1358_2112del |
| 2 | human | 10131 | TRAP1 | TNF receptor associated pro... | NM_001272049.2 | 56.6% | 54.8% | 87_88ins159;1155delA;1199_1953del |
| 3 | human | 10131 | TRAP1 | TNF receptor associated pro... | XM_011522345.2 | 44.3% | 42.4% | 0_1ins420;894delA;938_1692del |
| 4 | mouse | 68015 | Trap1 | TNF receptor-associated pro... | NM_026508.2 | 54.6% | 54.1% | (many diffs) |
| 5 | mouse | 68015 | Trap1 | TNF receptor-associated pro... | XR_001781856.1 | 46.9% | (many diffs) | |
| 6 | mouse | 68015 | Trap1 | TNF receptor-associated pro... | XR_001781855.1 | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1428
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgcgc gagctgcggg cgctgctgct gtggggccgc cgcctgcggc 121 ctttgctgcg ggcgccggcg ctggcggccg tgccgggagg aaaaccaatt ctgtgtcctc 181 ggaggaccac agcccagttg ggccccaggc gaaacccagc ctggagcttg caggcaggac 241 gactgttcag cacgcagacc gccgaggaca aggaggaacc cctgcactcg attatcagca 301 gcacagagag cgtgcagggt tccacttcca aacatgagtt ccaggccgag acaaagaagc 361 ttttggacat tgttgcccgg tccctgtact cagaaaaaga ggtgtttata cgggagctga 421 tctccaatgc cagcgatgcc ttggaaaaac tgcgtcacaa actggtgtct gacggccaag 481 cactgccaga aatggagatt cacttgcaga ccaatgccga gaaaggcacc atcaccatcc 541 aggatactgg tatcgggatg acacaggaag agctggtgtc caacctgggg acgattgcca 601 gatcggggtc aaaggccttc ctggatgctc tgcagaacca ggctgaggcc agcagcaaga 661 tcatcggcca gtttggagtg ggtttctact cagctttcat ggtggctgac agagtggagg 721 tctattcccg ctcggcagcc ccggggagcc tgggttacca gtggctttca gatggttctg 781 gagtgtttga aatcgccgaa gcttcgggag ttagaaccgg gacaaaaatc atcatccacc 841 tgaaatccga ctgcaaggag ttttccagcg aggcccgggt gcgagatgtg gtaacgaagt 901 acagcaactt cgtcagcttc cccttgtact tgaatggaag gcggatgaac accttgcagg 961 ccatctggat gatggacccc aaggatgtcc gtgagtggca acatgaggag ttctaccgct 1021 acgtcgcgca ggctcacgac aagccccgct acaccctgca ctataagacg gacgcaccgc 1081 tcaacatccg cagcatcttc tacgtgcccg acatgaaacc gtccatgttt gatgtgagcc 1141 gggagctggg ctccagcgtt gcactgtaca gccgcaaagt ccTCATCCAG ACCAAGGCCA 1201 CGGACATCCT GCCCAAGTGG CTGCGCTTCA TCCGAGGTGT GGTGGACAGT GAGGACATTC 1261 CCCTGAACCT CAGCCGGGAG CTGCTGCAGG AGAGCGCACT CATCAGGAAA CTCCGGGACG 1321 TTTTACAGCA GAGGCTGATC AAATTCTTCA TTGACCAGAG TAAAAAAGAT GCTGAGAAGT 1381 ATGCAAGTTT TTTGAAGATT ACGGCCTGTT CATGCGGGAG GGCATTGTGA GACCCAGCTT 1441 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1501 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1561 CTTGTGGAAA GGACGAATTT CAGTCGATCT CTCTACCCTC ACGCGTTAAG TCgacaatca 1621 acctctggat tacaaaattt gtgaaagatt