Construct: ORF TRCN0000488403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021216.1_s317c1
- DNA Barcode:
- CCTAAAAGTGCTCTCAATCCGGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NMUR1 (10316)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488403
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10316 | NMUR1 | neuromedin U receptor 1 | NM_006056.5 | 100% | 100% | |
| 2 | human | 10316 | NMUR1 | neuromedin U receptor 1 | XM_011510487.3 | 94.6% | 94.6% | 0_1ins69 |
| 3 | human | 10316 | NMUR1 | neuromedin U receptor 1 | XM_006712195.3 | 79.6% | 73% | (many diffs) |
| 4 | human | 10316 | NMUR1 | neuromedin U receptor 1 | XM_011510488.2 | 77.5% | 71.1% | (many diffs) |
| 5 | human | 10316 | NMUR1 | neuromedin U receptor 1 | XM_006712196.3 | 74% | 70.5% | (many diffs) |
| 6 | human | 10316 | NMUR1 | neuromedin U receptor 1 | XM_011510489.2 | 72.9% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1350
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgactcct ctctgcctca attgctctgt cctccctgga gacctgtacc 121 cagggggtgc aaggaacccc atggcttgca atggcagtgc ggccaggggg cactttgacc 181 ctgaggactt gaacctgact gacgaggcac tgagactcaa gtacctgggg ccccagcaga 241 cagagctgtt catgcccatc tgtgccacat acctgctgat cttcgtggtg ggcgctgtgg 301 gcaatgggct gacctgtctg gtcatcctgc gccacaaggc catgcgcacg cctaccaact 361 actacctctt cagcctggcc gtgtcggacc tgctggtgct gctggtgggc ctgcccctgg 421 agctctatga gatgtggcac aactacccct tcctgctggg cgttggtggc tgctatttcc 481 gcacgctact gtttgagatg gtctgcctgg cctcagtgct caacgtcact gccctgagcg 541 tggaacgcta tgtggccgtg gtgcacccac tccaggccag gtccatggtg acgcgggccc 601 atgtgcgccg agtgcttggg gccgtctggg gtcttgccat gctctgctcc ctgcccaaca 661 ccagcctgca cggcatccgg cagctgcacg tgccctgccg gggcccagtg ccagactcag 721 ctgtttgcat gctggtccgc ccacgggccc tctacaacat ggtagtgcag accaccgcgc 781 tgctcttctt ctgcctgccc atggccatca tgagcgtgct ctacctgctc attgggctgc 841 gactgcggcg ggagaggctg ctgctcatgc aggaggccaa gggcaggggc tctgcagcag 901 ccaggtccag atacacctgc aggctccagc agcacgatcg gggccggaga caagtgacca 961 agatgctgtt tgtcctggtc gtggtgtttg gcatctgctg ggccccgttc cacgccgacc 1021 gcgtcatgtg gagcgtcgtg tcacagtgga cagatggcct gcacctggcc ttccagcacg 1081 tgcacgtcat cTCCGGCATC TTCTTCTACC TGGGCTCGGC GGCCAACCCC GTGCTCTATA 1141 GCCTCATGTC CAGCCGCTTC CGAGAGACCT TCCAGGAGGC CCTGTGCCTC GGGGCCTGCT 1201 GCCATCGCCT CAGACCCCGC CACAGCTCCC ACAGCCTCAG CAGGATGACC ACAGGCAGCA 1261 CCCTGTGTGA TGTGGGCTCC CTGGGCAGCT GGGTCCACCC CCTGGCTGGG AACGATGGCC 1321 CAGAGGCGCA GCAAGAGACC GATCCATCCT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1501 TAAAAGTGCT CTCAATCCGG ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt