Construct: ORF TRCN0000488426

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019351.1_s317c1
DNA Barcode:
GCTACACTCCTGGTGTAAGCTGGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GPR52 (9293)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488426
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9293 GPR52 G protein-coupled receptor 52 NM_005684.5 99.8% 99.7% 105T>C;1083_1084insG
2 mouse 620246 Gpr52 G protein-coupled receptor 52 NM_001146330.1 91.3% 95.3% (many diffs)
3 mouse 620246 Gpr52 G protein-coupled receptor 52 XM_017321886.1 91.3% 95.3% (many diffs)
4 mouse 620246 Gpr52 G protein-coupled receptor 52 XM_017321888.1 91.3% 95.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1158
ORF length:
1086
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaatgaa tccaggtgga ctgaatggag gatcctgaac atgagcagtg
121gcattgtgaa tgtgtccgag cgtcactcct gcccacttgg atttggccac tacagcgtgg
181tggatgtctg catcttcgag acagtggtta ttgtgttgct gacatttctg atcattgctg
241ggaatctaac agttatcttt gtctttcatt gtgctccact gttacatcat tatactacca
301gctatttcat tcagacgatg gcatatgctg atcttttcgt tggagttagc tgcttggttc
361ctactctgtc acttctccac tactccacag gtgtccacga gtcattgact tgccaggttt
421ttggatatat catctcagtt ctaaaaagtg tttctatggc atgtcttgct tgcatcagtg
481tggatcgtta tcttgcaata accaagcctc tttcctacaa tcaactggtc accccttgtc
541gcttgagaat ttgcattatt ttgatctgga tctactcctg cctaattttc ttgccttcct
601tttttggctg ggggaaacct ggttaccatg gtgacatttt tgaatggtgt gccacgtctt
661ggctcaccag tgcctatttt actggcttta ttgtttgttt actttatgct cctgctgcct
721ttgttgtctg cttcacttac ttccacattt tcaaaatttg ccgtcagcac accaaagaga
781taaatgaccg aagagcccga ttccctagtc atgaggtaga ttcttccaga gagactggac
841acagcccTGA CCGTCGCTAC GCCATGGTTT TGTTTAGGAT AACCAGTGTA TTTTATATGC
901TGTGGCTCCC CTATATAATT TACTTTCTTC TAGAAAGCTC CCGGGTCTTG GACAATCCAA
961CTCTGTCCTT CTTAACAACC TGGCTTGCAA TAAGTAATAG TTTTTGTAAC TGTGTAATAT
1021ACAGCCTCTC CAACAGCGTT TTCCGGCTAG GCCTCCGAAG ACTGTCTGAG ACAATGTGCA
1081CATCCTGTAT GTGTGTGAAG GATCAGGAAG CACAAGAACC CAAACCTAGG AAACGGGCTA
1141ATTCTTGCTC CATTGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1201CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1261TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCTACACTCC TGGTGTAAGC
1321TGGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)