Construct: ORF TRCN0000488441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020132.1_s317c1
- DNA Barcode:
- CCAAACACAGTAAAAGCTACAGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GALK1 (2584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2584 | GALK1 | galactokinase 1 | NM_000154.2 | 99.9% | 100% | 189G>A |
| 2 | mouse | 14635 | Galk1 | galactokinase 1 | NM_016905.2 | 85.4% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1248
- ORF length:
- 1176
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgct ttgagacagc cccaggtcgc ggagctgctg gccgaggccc 121 ggcgagcctt ccgggaggag ttcggggccg agcccgagct ggccgtgtca gcgccgggcc 181 gcgtcaacct catcggggaa cacacggact acaaccaggg cctggtgctg cctatggctc 241 tggagctcat gacggtgcta gtgggcagcc cccgcaagga tgggctggtg tctctcctca 301 ccacctctga gggtgccgat gagccccagc ggctgcagtt tccactgccc acagcccagc 361 gctcgctgga gcctgggact cctcggtggg ccaactatgt caagggagtg attcagtact 421 acccagctgc ccccctccct ggcttcagtg cagtggtggt cagctcagtg cccctggggg 481 gtggcctgtc cagctcagca tccttggaag tggccacgta caccttcctc cagcagctct 541 gtccagactc gggcacaata gctgcccgcg cccaggtgtg tcagcaggcc gagcacagct 601 tcgcagggat gccctgtggc atcatggacc agttcatctc acttatggga cagaaaggcc 661 acgcgctgct cattgactgc aggtccttgg agaccagcct ggtgccactc tcggacccca 721 agctggccgt gctcatcacc aactctaatg tccgccactc cctggcctcc agcgagtacc 781 ctgtgcggcg gcgccaatgt gaagaagtgg cccgggcgct gggcaaggaa agcctccggg 841 aggtacaact ggaagagcta gaggctgcca gggacctggt gagcaaagag ggcttccggc 901 gggcccggca cgtggtgggg gagattcggc gcacggccca ggcagcggcc gcccTGAGAC 961 GTGGCGACTA CAGAGCCTTT GGCCGCCTCA TGGTGGAGAG CCACCGCTCA CTCAGAGACG 1021 ACTATGAGGT GAGCTGCCCA GAGCTGGACC AGCTGGTGGA GGCTGCGCTT GCTGTGCCTG 1081 GGGTTTATGG CAGCCGCATG ACGGGCGGTG GCTTCGGTGG CTGCACGGTG ACACTGCTGG 1141 AGGCCTCCGC TGCTCCCCAC GCCATGCGGC ACATCCAGGA GCACTACGGC GGGACTGCCA 1201 CCTTCTACCT CTCTCAAGCA GCCGATGGAG CCAAGGTGCT GTGCTTGTGA GACCCAGCTT 1261 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1321 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1381 CTTGTGGAAA GGACGACCAA ACACAGTAAA AGCTACAGCC ACGCGTTAAG TCgacaatca 1441 acctctggat tacaaaattt gtgaaagatt