Construct: ORF TRCN0000488442
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020122.1_s317c1
- DNA Barcode:
- ATTCGCCAACATTATGCCGAGTGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPK1 (5594)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488442
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5594 | MAPK1 | mitogen-activated protein k... | NM_002745.4 | 99.7% | 99.4% | 3_5delGGC |
| 2 | human | 5594 | MAPK1 | mitogen-activated protein k... | NM_138957.3 | 99.7% | 99.4% | 3_5delGGC |
| 3 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | NM_001038663.1 | 92.5% | 98.8% | (many diffs) |
| 4 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | NM_011949.3 | 92.5% | 98.8% | (many diffs) |
| 5 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | XM_006522147.3 | 92.5% | 98.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1149
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggcg gcggcggcgg gcgcgggccc ggagatggtc cgcgggcagg 121 tgttcgacgt ggggccgcgc tacaccaacc tctcgtacat cggcgagggc gcctacggca 181 tggtgtgctc tgcttatgat aatgtcaaca aagttcgagt agctatcaag aaaatcagcc 241 cctttgagca ccagacctac tgccagagaa ccctgaggga gataaaaatc ttactgcgct 301 tcagacatga gaacatcatt ggaatcaatg acattattcg agcaccaacc atcgagcaaa 361 tgaaagatgt atatatagta caggacctca tggaaacaga tctttacaag ctcttgaaga 421 cacaacacct cagcaatgac catatctgct attttctcta ccagatcctc agagggttaa 481 aatatatcca ttcagctaac gttctgcacc gtgacctcaa gccttccaac ctgctgctca 541 acaccacctg tgatctcaag atctgtgact ttggcctggc ccgtgttgca gatccagacc 601 atgatcacac agggttcctg acagaatatg tggccacacg ttggtacagg gctccagaaa 661 ttatgttgaa ttccaagggc tacaccaagt ccattgatat ttggtctgta ggctgcattc 721 tggcagaaat gctttctaac aggcccatct ttccagggaa gcattatctt gaccagctga 781 accacatttt gggtattctt ggatccccat cacaagaaga cctgaattgt ataataaatt 841 taaaAGCTAG GAACTATTTG CTTTCTCTTC CACACAAAAA TAAGGTGCCA TGGAACAGGC 901 TGTTCCCAAA TGCTGACTCC AAAGCTCTGG ACTTATTGGA CAAAATGTTG ACATTCAACC 961 CACACAAGAG GATTGAAGTA GAACAGGCTC TGGCCCACCC ATATCTGGAG CAGTATTACG 1021 ACCCGAGTGA CGAGCCCATC GCCGAAGCAC CATTCAAGTT CGACATGGAA TTGGATGACT 1081 TGCCTAAGGA AAAGCTCAAA GAACTAATTT TTGAAGAGAC TGCTAGATTC CAGCCAGGAT 1141 ACAGATCTTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1201 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1261 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATT CGCCAACATT ATGCCGAGTG 1321 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t